Immunologi

This open access book explores techniques for working in the field of immunogenetics, i.e. fundamental and translational research into the adaptive immune receptor repertoire. Many chapters are dedicated to lab protocols, bioinformatics, and immunoinformatics analysis of high-resolution immunome analysis, exemplified by numerous applications. Additionally, the newest technological variations on these protocols are discussed, including non-amplicon, single-cell, and cell-free strategies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Immunogenetics: Methods and Protocols covers a broad spectrum of methodologies for applications in research and clinical diagnostics to illustrate the impact that immunogenetics has achieved and will further expand in all fields of medicine, from infection and (auto)immunity, to vaccination, to lymphoid malignancy and tumor immunity.

Microbiota Brain Axis: A Neuroscience Primer provides neuroscience researchers with a comprehensive guide on how to conduct effective microbiota-brain research, understand the appropriate methodologies, and collect and analyze microbiota data. The book begins with an introduction to the importance of the microbiota-brain communication in development and how microbiota impact neurodevelopmental disorders, mental health and neurodegeneration. In addition, the book discusses advances in microbiota analysis tools and techniques for neuroscience related research.

This easy to digest consumer guide explains the relationship between the foods we eat and how they can lead to one of over 100 autoimmune disorders.Elroy Vojdani MD, IFMCP, is revered by his peers and patients for his ability to get to the root cause of their chronic illness(es).The book guides the reader through how to detect the source of the autoimmune disorder, remove the triggers and then heal the body.Nearly 1 in 6 Americans experience autoimmunity symptoms. Between 75-80% of of those afflicted are women. According to the National Institutes of Health (NIH), 25.3 million Americans currently live with an autoimmune disorder. This figure doesn't include people who go undiagnosed or are misdiagnosed.More than 70% of autoimmune disorders can be traced back to consuming the wrong foods, making When Food Bites Back a must-have.

Why can two people use a drug and one person becomes addicted while the other does not? Determinants of Addiction: Neurobiological, Behavioral, Cognitive, and Sociocultural Factors unravels the complexities underlying addiction to understand how individual factors at the genetic, cellular, anatomical, cognitive-behavioral, and sociocultural level can influence susceptibility to substance use disorders. The first section reviews the neurobiological determinants of addiction and examines how drugs hijack the reward pathway and alter numerous neurotransmitter systems such as dopamine. The second section covers the behavioral-cognitive determinants of addiction such a conditioning, memory processes, and decision-making. The final section examines individual differences in addiction vulnerability, with a focus on personality factors, sociocultural factors, sex/gender, and stress. The book references commonly used drugs such as nicotine, ethanol (alcohol), opioids, and cocaine.

This volume looks at in vitro disease models representing the respiratory, hepatobiliary, osteochondral, nervous, dermal, ocular, immune system, and pathological biological processes like tumorigenesis for stem cell research. The chapters in this book cover a range of diseases and application of various stem cells such as adult stem cells and iPS. Chapters also discuss new methods to characterize and manipulate stem cells with the aim to better understand and improve their biological performance. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and thorough, In Vitro Models for Stem Cell Therapy: Methods and Protocols is a valuable resource for researchers and scientists interested in learning more about this exciting field

This second edition of Eosinophils: Methods and Protocols updates several techniques from the first edition together with novel in vitro and in vivo-based methodologies. Written by internationally recognized expert authorities, this volume provides vital techniques from eosinophil purification to experimental modelling, with each technique spelled out in clear and straightforward terms, assuming no previous knowledge of the method and without necessitating sourcing additional information elsewhere. Written in the highly successful Methods in Molecular Biology format, chapters include introductions to their respective topics, lists of the essential materials and reagents, step-by-step, readily reproducible laboratory protocols, with tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Eosinophils: Methods and Protocols, Second Edition provides the practical means to extend our knowledge of eosinophil function in health and disease, underpinning research that may in turn lead to new hypotheses for future examinations into the role of this intriguing and enigmatic leukocyte.Chapters 10 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

This second edition provides new and updated chapters useful for the study of Regulatory B cells. Organized in four sections, chapters detail basic methods for the isolation and immunophenotypical analysis of these cells, experimental approaches for the ex vivo generation/expansion of IL-10 producing B cells, molecular biology techniques for the analysis of IL-10 expression and production, and animal models mimicking pathologic settings. Written in the highly successful Methods in Molecular Biology series format, chapters include an introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Regulatory B Cells: Methods and Protocols, Second Edition aims to be useful to the scientific community and serve toclarify some unsolved aspects of Regulatory B Cells research.

This textbook provides a comprehensive overview of the state of the art in otolaryngology, discussing all the newly advances in the subspecialties of head and neck, plastics, otology, laryngology, rhinology and pediatrics, and also addressing topics like allergy, sleep medicine, trauma, and the fundamentals of systemic diseases that frequently manifest in the head and neck region.The book is divided into 9 sections, presenting the recent literature concerning all the subspecialties in otolaryngology and providing the information necessary for readers to gain an understanding of the field of otolaryngology.Each chapter includes definitions, key points and take-home messages, to aid learning. Throughout the book, tips and key features are highlighted with boxes, tables and figures, which the reader can refer back to for quick revision. Above all, the book enables medical students, residents and junior specialists in the field of ENT to develop their learning and surgical skills.

This book focuses on cell culture-produced viral vaccines to meet the needs of the rapidly expanding research and development in academia and industry in the field. This book introduces the basic principles of vaccination and the manufacturing of viral vaccines. Bioprocessing of Viral Vaccines, will provide an overview of the advanced strategies needed to respond to the challenges of new and established viral infection diseases. The first few chapters cover the basics of virology and immunology as essential concepts to understand the function and design of viral vaccines. The core of the content is dedicated to process development, including upstream processing and cell culture of viral vaccines, downstream processing, and extensive analytical technologies specific to viral vaccines. Advanced process analytical technologies (PAT) and Quality by Design (QbD) concepts are also introduced in the context of vaccine manufacturing. The case studies included cover inactivated, attenuated vaccines exemplified by influenza vaccines, sub-unit vaccines exemplified by Virus Like Particles (VLPs: HPV vaccines) and sub-unit vaccines (Flublock), vectored vaccines: adenoviruses and Vesicular stomatitis Virus (VSV) vectored vaccines, genomic vaccines (DNA and mRNA) vaccines as developed for COVID-19 response in particular and a review of COVID-19 vaccines approved or in advanced clinical trials. This book is aimed at graduate engineers and professionals in the fields of vaccinology, bioprocessing, and biomanufacturing of viral vaccines.

This book updates in detail the microbial pathogenesis of various important pathogens, including HIV-1, MERS, SARS-CoV-2, Mycobacterium and Plasmodium. There is also a general discussion of the innate and adaptive immune responses against primary and opportunistic infections. The overall purpose of the book is to aid in the development of anti-viral and anti-microbial targets.

Skin physiology assessment is moving from a descriptive approach to a deeper understanding of biophysical and biochemical processes in the stratum corneum, such as epidermal barrier function and stratum corneum hydration. New, non-invasive approaches offer reliable and reproducible methods for product testing in the pharmaceutical and cosmetic industry, as well as in basic research. While standard instruments focus on functional aspects, innovative devices offer a deeper understanding of underlying mechanisms.This book discusses the assessment of skin physiology and of skin functions in clinical studies using non-invasive biophysical instruments, offering readers a comprehensive guide to planning, performing and evaluating the results of scientific studies in skin measurement and the legal framework for these studies. Written by leading experts in the field, it focuses on practical aspects of non-invasive measurements. After introducing the legal aspects of the current framework for clinical cosmetic studies and basic research in cosmetology, it explores the technical practicalities of organizing a testing lab and the pre-requirements for planning a study. The third and main section addresses specific topics in cosmetic testing e.g. skin hydration, and also includes chapters on sensory aspects and in vivo skin structure vizualization.    This new, updated edition of Practical Aspects of Cosmetic Testing is a valuable tool for researchers, students, and medical staff wanting to gain insights into how best to assess skin functions in controlled studies using non-invasive biophysical instruments.

Ce que le vulgaire appelle les Fièvres, ou encore, en précisant davantage les Fièvres des marais, les médecins l'appellent le Paludisme. Le mot évoque, dans les souvenirs de chacun, le spectacle d'un malheureux en proie au frisson qui secoue ses membres et fait claquer ses dents, tandis qu'au tableau suivant, le feu de la fièvre brûlera son corps et, à la fin de la crise, l'inondera d'une abondante sueur. Cette scène, - et c'est là l'un de ses caractères les plus remarquables, - se reproduit périodiquement, avec une régularité surprenante, à la même heure tous les jours, ou tous les deux jours, ou tous les trois jours (fièvre quotidienne, tierce, quarte). Mais c'est là une image simplifiée et trop nette. En réalité, une telle fièvre, franchement intermittente, rigoureusement périodique, entrecoupée de rémissions presque complètes, ne représente que l'une des formes sous lesquelles se manifeste la maladie. Il y en a bien d'autres, que connaît le médecin expérimenté. Les accès, en effet, peuvent être réguliers : la fièvre peut être continue ; elle peut être rémittente ; elle peut être larvée ; elle est quelquefois mortelle dès le premier ou le second accès (fièvre pernicieuse)...

This second edition volume expands on the previous edition with new sections describing the characterization of peptides bound to major histocompatibility complexes (MHC) on the surface of the cell. Chapters also cover topics such as using SERPA for antigen identification; antigen content of electroimmunoprecipitates; whole genome-phage display libraries; antigens in immune complexes; and immunoproteomic biomarkers. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Immunoprotemics: Methods and Protocols, Second Edition is a valuable resource that presents novice and expert researchers with techniques that are easily transferrable to laboratories and provides enhancedhands-on insights into this evolving field.