Gør som tusindvis af andre bogelskere
Tilmeld dig nyhedsbrevet og få gode tilbud og inspiration til din næste læsning.Ved tilmelding accepterer du vores persondatapolitik.
Du kan altid afmelde dig igen.
The Song of the Cell is the vivid, thrilling and suspenseful story of the fundamental unit of life. It describes how scientists discovered cells, began to understand them, and are now using that knowledge to create new humans. Both panoramic and intimate, it is Siddhartha Mukherjee's most spectacular book yet.In the late 1600s, a distinguished English polymath, Robert Hooke, and an eccentric Dutch cloth-merchant, Antonie van Leeuwenhoek, look down their hand-made microscopes. What they see introduces a radical concept that alters both biology and medicine forever. It is the fact that complex living organisms are assemblages of tiny, self-contained, self-regulating units. Our organs, our physiology, our selves, are built from these compartments. Hooke christens them 'cells'.The discovery of cells announced the birth of a new kind of medicine. A hip fracture, a cardiac arrest, Alzheimer's, AIDS, lung cancer - all could be re-conceived as the results of cells, or a cellular ecosystem, functioning abnormally. And all could be treated by therapeutic manipulations of cells.This revolution in cell biology is still in progress: it represents one of the most significant advances in science and medicine.Rich with stories of scientists, doctors, and the patients whose lives may be saved by their work, The Song of the Cell is the third book in this extraordinary writer's exploration of what it means to be human.
Dyrkning af mammale celler in vitro bruges til utallige formål, fx:DiagnostikCancerforskningProduktion af virusProduktion af farmakonProduktion af monoklonale antistofferBiokemiske og biologiske undersøgelserBehandling af patienterBogen beskriver i detaljer teknikkernes fælles grundlag: indretning af celledyrkningslaboratorium, korrekt brug af apparatur, sterilisation, sikkerhed og affaldshåndtering, infektioner med mikroorganismer, krydskontaminering, medier, vedhæftning af celler til overflader, subkultivering, vækstkurver, kvantitiering, nedfrysning/optøning og kloning/selektion.Bogen henvender sig til celledyrkere og giver svar på spørgsmål, der opstår i det praktiske arbejde med celledyrkning. Bogen kan derfor bruges som opslagsbog i det daglige arbejde i laboratoriet.
This book describes the shape formation of living organisms using mathematical models. Genes are deeply related to the shape of living organisms, and elucidation of a pathway of shape formation from genes is one of the fundamental problems in biology. Mathematical cell models are indispensable tools to elucidate this problem. The book introduces two mathematical cell models, the cell center model and the vertex model, with their applications. The cell center model is applied to elucidate the formation of neat cell arrangements in epidermis, cell patterns consisting of heterogeneous-sized cells, capillary networks, and the branching patterns of blood vessels. The vertex model is applied to elucidate the wound healing mechanisms of the epithelium and ordered pattern formation involving apoptosis. Pattern formation with differential cell adhesion is also described. The vertex model is then extended from a two-dimensional (2D) to a three-dimensional (3D) model. A cell aggregate involving a large cavity is described to explain the development of the mammalian blastocyst or the formation of an epithelial vesicle. Epithelial tissues and the polarity formation process of the epithelium are also explained. The vertex model also recapitulates active remodeling of tissues and describes the twisting of tissue that contributes to understanding the cardiac loop formation of the embryonic tube. The book showcases that mathematical cell models are indispensable tools to understand the shape formation of living organisms. Successful contribution of the mathematical cell models means that the remodeling of collective cells is self-construction. Examining the successive iterations of self-constructions leads to understanding the remarkable and mysterious morphogenesis that occurs during the development of living organisms. The intended readers of this book are not only theoretical or mathematical biologists, but also experimental and general biologists, including undergraduate and postgraduate students who are interested in the relationship between genes and morphogenesis.
This book highlights the application of microfluidics in cell biology research, chemical biology, and drug discovery. It covers the recent breakthroughs and prospects of organ-on-a-chip, human-on-a-chip, multi-organ-on-a-chip for personalized medicine. The book presents the preclinical studies of organs-on-a-chip, concepts of multiple vascularized organ-on-chips, application of organ-on-a-chip in blood-brain barrier model, culture and co-culture of cells on multi-organ-on-chip and parameter measurements in microfluidic devices. It underscores the advantage of microfluidic devices for developing efficient drug carrier particles, cell-free protein synthesis systems, and rapid techniques for direct drug screening. Further, it entails human-on-a-chip for measuring the systemic response as well as immediate effects of an organ reaction on other organs. In summary, this book reviews the development of a microfluidic-based organ-on-a-chip device for the preclinical evaluation, ADME studies of drugs, chemicals, and medical devices. This book is a valuable source for pharma companies, product developers, students, researchers, academicians, and practitioners.
This book focuses on in vitro techniques and challenges of producing nutraceutical compounds from plant cells. In addition, it provides an overview of different biosynthesis pathways and their modulation through cell culture techniques for the production of nutraceutical compounds in high quantity and quality. It also includes the assessment of the factors influencing production and advances in cell culture techniques, including the scale-up approach using bioreactors. Lastly it provides valuable suggestion for future research.
This edited book highlights the plant and cell/organ culture systems, and environmental and genetic transformation-based modulation of biochemical pathways. Special focus is given to microRNA-based technology, heterologous systems expression of enzymes and pathways leading to products of interest, as well as applications using both model and non-model plant species.Metabolic engineering is usually defined as the re-routing of one or more enzymatic reactions to generate new compounds, increase the production of existing compounds, or facilitate the degradation of compounds. Plants are the foundation of numerous compounds which are synthesized via assimilated complex biosynthetic routes. Plants have evolved an incredible arrangement of metabolic pathways leading to molecules/compounds capable of responding promptly and effectively to stress situations imposed by biotic and abiotic factors, some of which supply the ever-growing needs of humankind for natural chemicals, such as pharmaceuticals, nutraceuticals, agrochemicals, food and chemical additives, biofuels, and biomass. However, in foreseeable future we will be forced to think about the accessibility of resources for the generations to come. For these reasons, the book proposes alternative options of food/food supplement, medicines and other essential items, by using plant metabolic engineering approach. This book is of interest to teachers, researchers and academic experts. Also, the book serves as additional reading material for undergraduate and graduate students of biotechnology and molecular biology of plants.
This comprehensive, up-to-date reference work compiles the scientific research on the relationship between various forms of cancer and reactive oxygen species (ROS). It is well known that ROS play a key role in the various steps leading to malignancy, this includes increase in DNA mutation rates. The book brings together exciting reviews, written by leading experts all over the world.The book is divided into four broad sections. Each section describes the role of oxidative stress and underlying mechanisms and pathways, at various stages of cancer, including its role in carcinogenesis, cancer progression and metastasis. Further, the book describes how the reactive oxygen species (ROS) modulate gene expression and signal transduction pathways in cancer. The book discusses the ways to interfere with the cancer progression, and design of anti-cancer drugs. The book provides an integrated approach for better understanding of the development phases of oxidative stress-induced cancers, and mainly the current perspectives about mechanistic aspects of cancer initiation, progression and metastasis.It is a highly resourceful book and would be extremely important to clinicians, students and researchers in the field of cancer genetics, therapeutics and oncology.
Much research has focused on the basic cellular and molecular biological aspects of stem cells. Much of this research has been fueled by their potential for use in regenerative medicine applications, which has in turn spurred growing numbers of translational and clinical studies. However, more work is needed if the potential is to be realized for improvement of the lives and well-being of patients with numerous diseases and conditions.This book series 'Cell Biology and Translational Medicine (CBTMED)' as part of Springer Nature's longstanding and very successful Advances in Experimental Medicine and Biology book series, has the goal to accelerate advances by timely information exchange. Emerging areas of regenerative medicine and translational aspects of stem cells are covered in each volume. Outstanding researchers are recruited to highlight developments and remaining challenges in both the basic research and clinical arenas. This current book is the 16th volume of a continuing series.Chapter "e;Epithelial Stem Cells: Making, Shaping and Breaking the Niche"e; is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
This book explores the importance of Single Nucleotide Polymorphisms (SNPs) in biomedical research. As SNP technologies have evolved from labor intensive, expensive, time-consuming processes to relatively inexpensive methods, SNP discovery has exploded. In terms of human biology, this research, particularly since the completion of the Human Genome Project, has provided a detailed understanding of evolutionary forces that have generated SNPs. It also has shown how SNPs shape human variation. The ability to inexpensively generate and analyze vast amounts of genetic data is poised to transform our understanding of human evolution and biology. "e;Single Nucleotide Polymorphisms"e; covers a broad survey of SNPs and their classification into synonymous and non-synonymous; the role of SNPs in human disease; case studies providing specific examples of synonymous and non-synonymous SNPs associated with human diseases or affecting therapeutic interventions; mechanisms by which synonymous mutations affect protein levels or protein folding which affect human physiology and response to therapy; and the role of SNPs in personalized medicine. Understanding what SNPs are, how they have been shaped is necessary for an increasingly expanding audience. This research will revolutionize the future of medicine. Chapter 4 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com. SNPs Ability to Influence Disease Risk: Breaking the Silence on Synonymous Mutations in Cancer"e; is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
This new volume, a part of the Proteomics, Metabolomics, Interactomics and Systems Biology series, will explain how proteomic studies of post-translational modifications (PTMs) can be applied to neurodegenerative diseases and relevant studies. The goal of the book is to increase awareness among researchers about how PTMs may be helpful in understanding mechanisms in various neurodegenerative diseases through proteomic studies. This book will serve as a tool for those who want to begin work in the proteomics field and explore how to implement PTMs studies into their work. Chapter authors will describe different PTMs enrichment methods developed by experts in the field so that researchers may learn to apply these methods and techniques to new studies. Divided into three sections, chapters will cover sample preparation, data quality, enrichment techniques, guidelines on how to analyze PTMs, and explain the role of PTMs and different brain diseases. Among those topics includes will be brain cancer, SLA disease, Parkinsons disease, muscular dystrophies, and schizophrenia. This volume will be useful for researchers and students studying brain and neurodegenerative diseases who are interested in delving into work with proteomic studies and PTMs.
The major purpose of this book is to present Johann Gregor Mendel (1822-1884) in a real and interesting way based on the most recent historical research and analysis of authentic sources. The authors aim to show Mendel's scientific thinking and inner feelings together with his environment and to communicate his message as a multifaceted personality and modern experimentalist.The book draws from the only existing short sketch of Mendel's youth, his letters and the biographical ceiling paintings that were made according to his proposal. They form the basis of the self-portrait concept. The structure of the book follows thematic groups covering Mendel's activities from a poor village boy in search for education and financial security, as not being physically suitable for running his father's farm. The book does not perpetuate the myths invented by some creative authors to make Mendel's biography more attractive. Mendel's life and work are dramatic enough without those embellishments. Mendel found happiness in science and he was able to explain the theory of new scientific facts. He was not a tragic figure, he did not work to become famous, but to be useful. His pea research has now been appreciated as a genius accomplishment of a scientist. The book is published at the occasion of Mendel's birthday bicentennial.
This volume covers a broad range of cell types including cultured cell lines, primary cells, and various unicellular organisms such as fission yeast, budding yeast, parasite Leishmania amazonensis, and parasite Trypanosoma brucei. The chapters in this book are organized into four parts. Part One looks at a general overview of cell cycle control and synchronization. Part Two discusses techniques to synchronize mammalian cells to various cell cycle phases including mitotic sub-phases. Part Three covers synchronization of unicellular organisms and Part Four analyzes cell cycle progression. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Cell-Cycle Synchronization: Methods and Protocols is a valuable resource for both novice and expert scientists in this developing field.
This volume details comprehensive protocols and methodologies to assess mitochondrial bioenergetics and dynamics in different tissues and cells involving health and pathological states. Chapters guide readers through methods for assessment of the energy metabolism including Oxygen Consumption Rate (OCR), mitochondrial membrane potential, and measuring mitochondrial Ca2+ handling, and ROS emission. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting, and systematic reproducible protocols. Authoritative and cutting-edge, Mitochondria: Methods and Protocols aims to be a foundation for future studies and to be a source of inspiration for new investigations in the field.
This volume covers comprehensive methods on ways to assess structural and ultrastructural changes in the mitochondria, cytoskeleton, and microglia using state-of-the-art microscopy techniques including super-resolution imaging, electron microscopy, and ultra-high field MRI. The chapters in this book cover topics such as analysis of neurodegeneration in the post-mortem characterization of preclinical animal models, in vivo modeling in cell death in different model systems and brain organoids, single cell clonal analysis using Mosaic Analysis with Double Markers in genetic mouse models, and genome and proteomic methods for analysis of mRNA dynamics and quantitation of targeted peptides. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and thorough, Neuronal Cell Death: Methods and Protocols is a valuable resource for any scientist and researcher interested in learning more about this developing field.
This fully updated volume explores improved and extended protocols for embryonic stem cell (ESC) research, provided with the most direct and informative methodologies currently available. The book examines how these models for cell lineage and differentiation studies have continued to mature into a critical research workhorse. Written for the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Embryonic Stem Cell Protocols, Fourth Edition serves as an ideal resource for researching mining the depths of the ESC field.
This volume provides detailed protocols for the identification and understanding of vasculogenic mimicry process in vitro and in vivo, in addition to protocols for microscopy and histology. Chapters guide readers through different materials, commercial and homemade scaffolds, Matrigel, cancer spheroids, 3D tissue constructs, vasculogenic processes, and mathematical model building. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Vasculogenic Mimicry: Methods and Protocols aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.
This detailed volume presents a series of methods exploring membrane trafficking research, ranging from genetics and high-resolution imaging to in vitro biochemical and biophysical assays. Covering virtually all the major trafficking branches, the book delves into the exocytic pathway, which focuses on cargo transport from the ER to the Golgi, through the Golgi cisternae, and to the plasma membrane and the extracellular space; the endocytic pathway, which includes cargo endocytosis, endosomal recycling, and lysosomal degradation; as well as emerging topics beyond the conventional exocytic and endocytic pathways. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Membrane Trafficking: Methods and Protocols provides techniques with broad applications as an ideal guide for junior researchers new to membrane trafficking as well as established membrane biologists seeking to expand their research programs.
This detailed edition reflects the significant new findings in the components of permeability barriers and how they work in different tissues with a collection of cutting-edge techniques. Chapters explore the formation, maintenance, regulation, and dynamics of permeability barriers in an effort to push the boundaries of the field. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Permeability Barrier: Methods and Protocols, Second Edition serves as an invaluable guide for both experts but novices in the stem cell field and other related areas of research.
This volume of the Encyclopedia of Complexity and Systems Science, Second Edition, focuses on current challenges in the field from materials and mechanics to applications of statistical and nonlinear physics in the life sciences. Challenges today are mostly in the realm of non-equilibrium systems, although certain equilibrium systems also present serious hurdles. Where possible, pairwise articles focus on a single topic, one from a theoretical perspective and the other from an experimental one, providing valuable insights. In other cases, theorists and experimentalists have collaborated on a single article. Coverage includes both quantum and classical systems, and emphasizes 1) mature fields that are not covered in the current specialist literature, (2) topics that fall through the cracks in disciplinary journals/books, or (3) developing areas where the knowledge base is large and robust and upon which future developments will depend. The result is an invaluable resource for condensed matter physicists, material scientists, engineers and life scientists.
An overview of photosynthetic and respiratory mechanisms in plants under hostile situations and the proper mitigating strategies to continue uninterrupted photosynthesis and respiration under such situations. It looks at the effect of environmental stressors on plants.
Initially believed to be inactive molecules, glycans are now considered essential for life, both under normal and pathological conditions. This volume of the series "Biology of Extracellular Matrix" reviews the most recent findings on the role of glycans in the development of diseases and the possible therapeutic use of this class of molecules. It shows how the interaction of glycans with growth factors, growth factor binding proteins, extracellular proteases, protease inhibitors, chemokines, morphogens, and adhesive proteins regulates inflammation, infection, cancer, atherosclerosis, thrombosis and embryonic stem cell biology. Furthermore, an extensive survey about the structure and pharmacological effects of unique marine glycosaminoglycans is discussed as well as the possibility of using these glycans as therapeutic agents.