Bøger udgivet af Cold Spring Harbor Laboratory Press,U.S.

Cancer occurs because cells develop mutations that confer a growth advantage. Different subpopulations within the tumor can possess different mutations, and new mutations occur as the cancer progresses. This can allow the tumor to develop resistance to chemotherapy and is a major therapeutic challenge. This book examines this quasi-evolutionary process involved and how work in both molecular and computational biology is shedding light on it.

Cilia and flagella are protrusive structures on the surface of cells that perform a variety of functions. The beating of flagella and motile cilia, for example, can be used to propel cells or move material over their surface, and cilia play important roles in signaling between cells. This book examines the structure and function of cilia and flagella and their roles in cell motility, signaling and development.

Ion channels convey ions such as potassium, sodium and calcium across cellular membranes and play critical roles in normal physiology. This laboratory manual is based on the popular Cold Spring Harbor Laboratory course that has run for many years. It includes state-of-the-art techniques for analysis of ion channel activity and function.

Restriction enzymes cleave DNA at specific recognition sites and have many uses in molecular biology, genetics, and biotechnology. More than 4000 restriction enzymes are known today, of which more than 621 are commercially available, justifying their description by Nobel Prize winner Richard Roberts as "the workhorses of molecular biology." This book by Wil Loenen is the first full-length history of these invaluable tools, from their recognition in the 1950s to the flowering of their development in the 1970s and 1980s to their ubiquitous availability today. She is the author of several authoritative and widely appreciated reviews of the enzymes' biology.

The 80th Cold Spring Harbor Symposium was held to mark the 150th anniversary of Gregor Mendel's landmark 1865 presentation of his paper "Experiments on Plant Hybridization", which laid the groundwork for modern genetics. The Symposium Proceedings addresses 21st Century Genetics: Genes at Work, and provides a current synthesis of genetic mechanisms and genome/chromosome biology. This volume spans a broad range of topics that reflect our current understanding of genetic mechanisms in humans and other organisms. Themes include chromosome biology and nuclear structure, topologically associating domains, gene-enhancer interactions, chromatin and epigenetics, gene regulation and control, developmental regulation, RNA controlling elements, maintenance of genome stability, nuclear receptors, circadian clocks and aging, and genome editing. The Conversations included in this volume are based on interviews conducted during the Symposium and offer a broader anecdotal perspective on this fascinating subject by many of the world's leading investigators.

The machinery that controls how DNA is packaged and genes are activated plays an important role in cancer. This book examines the functions of components of this machinery, how they can be dysregulated, and the prospects for targeting them therapeutically.

Genetics is the science of biological inference from regularities in patterns of inheritance. It is a fundamentally analytical enterprise. It has become clear, especially in the last 50 years, that many important things about biology can be learned using genetic analysis that cannot be learned any other way. Genetic analysis is the best window we have into the workings of evolution. This book is organized around the specialized words that capture the most fundamental genetic abstractions. It conveys the meaning and utility of the essential and helpful ones as they are used today.

Epilepsy affects millions worldwide and represents a variety of disorders characterized by seizures. This book examines the neurobiological bases of these conditions and current research into treatment strategies.

Mitosis - division of a cell to produce two daughter cells - is a complicated process that must be highly coordinated to ensure events such as chromosome separation take place at the right time and in the right order. This book provides a state-of-the-art examination of the mechanics of mitosis, how the process is regulated, and the different checkpoints and quality-control steps involved.

This book is a highly illustrated sketch of the history of the Lab's research and educational activities from rustic beginnings to world renown. It will contain an introduction by Bruce Stillman, and a main text by Jan Witkowski consisting of three elements: an illustrated essay about the research done at the Lab's founding institutions from 1890 through 1962; a second illustrated essay about the research discoveries and accomplishments in education from 1962 to the present day; a section of "vignettes," short, highly structured accounts of 40 highlights from the Lab's track record of research.

We learn and form memories by making changes to neurons and neuronal circuits in the brain. This book examines the molecular and cellular bases for these processes, as well as how memories are stored, maintained and retrieved.

Hepatitis B affects hundreds of millions of individuals worldwide and is a leading cause of liver cancer. This book examines our understanding of the biology of the Hepatitis B virus that causes the disease, the immune responses it elicits, and its role in liver cancer. It also discusses the related Hepatitis Delta virus and its effects.

The generation of new neurons is a complex process that represents an extreme example of neuroplasticity. It may play a part in memory and brain repair and is implicated in various neurological conditions. This book discusses current approaches used to investigate neurogenesis, our understanding of the molecular mechanisms involved, and the roles of neurogenesis in diseases such as epilepsy and various psychiatric disorders.

"A subject collection from Cold Spring Harbor Perspectives in Biology."

Fungal infections affect millions of individuals worldwide. They a particular danger to immunocompromised individuals, such as those with HIV/AIDS, and invasive infections often have a mortality rate greater than 50%. This book examines our understanding of the biology of the major fungal pathogens, together with the host response, epidemiology of fungal diseases and current treatment strategies.

Experimental Design for Biologists is a unique and successful handbook on the theory and practice of effective design of scientific experiments, based on a well-received course by the author. This second edition is entirely reorganized, rewritten, and includes new material and figures. The material is presented in seven parts: Philosophy of Scientific Experimentation, Mapping Out the Project, System Validation, Experimental Design, Examples, What Comes After, and Putting It All Together. Experimental Design for Biologists, Second Edition, is an essential source in designing a sound research plan, critical to the success of graduate students.

"Nobelist James D. Watson delves into his family history, exploring his ancestors' roots in Springfield, Illinois, and Chicago, and then focuses on his father James D. Watson, Sr., and his influence on Dr. Watsonp1ss success as an eminent scientist and as a writer. Contiguous people, such as Abraham Lincoln and Orson Welles, and events, such as the

Retinal disorders are a leading cause of blindness. This book examines the molecular basis of these diseases, focusing on genetic approaches that are improving both our understanding of the disease process and their diagnosis, as well as advances in gene therapy that have been used to cure patients and provide a model for treatment of other conditions.

The innate immune system is the first line of defense against infection. It is a generic rather than pathogen-specific response that recruits immune cells to sites of infection, promotes inflammation, and activates the adaptive immune system. This book examines the cell types that make up the innate immune system, the chemical signals they use to communicate, and the mechanisms that drive inflammation and other consequences of immune activation.

As universities increasingly spin off biotech companies and academics work more closely with these companies and the pharma industry, it is essential that they gain an understanding of intellectual property law and patenting. This book introduces the basic ideas and discusses how they apply to biomedical research and product development in the age of genomics.

Recent work has improved our understanding of the molecular and cellular bases of heart development and function. This book discusses these advances and the implications for diagnosis and treatment of heart disease, including stem cell therapy, tissue engineering, and transplantation.

Neuroscientists must now routinely use advanced molecular biology and tissue culture techniques in their research. This book provides a detailed practical guide to both fundamental and cutting-edge methods in these areas. Aimed specifically at neuroscientists, it includes protocols for working with DNA and RNA, cell purification and tissue culture, and microscopy, as well as numerous organism-specific techniques.