Bøger i Perspectives Cshl serien

Written and edited by experts in the field, this collection from Cold Spring Harbor Perspectives in Medicine examines next-generation sequencing technologies and their use, particularly in translational research. The contributors discuss the various sequencing platforms, their capabilities, and their applications in both research and clinical practice. The roles of next-generation sequencing in diagnosing autism and intellectual disabilities, monitoring cancers during disease progression, and determining the most appropriate drug treatments for patients are also covered.

Written and edited by experts in the field, this collection covers the recent progress that has been made in understanding the molecular mechanisms underlying metastasis and how this knowledge is paving the way for new therapeutic targets. The contributors discuss progress in defining mutations that might be driving metastasis, the formation of a premetastatic niche and how it enhances the establishment of a secondary tumor, and how changes that occur during aging increase the metastatic capacity of tumor cells.

Written and edited by experts in the field, this collection from Cold Spring Harbor Perspectives in Biology describes recent progress in our understanding of early heart development and the various cell lineages involved, as well as mechanisms and models of congenital heart disease. The contributors discuss early cardiac morphogenesis and anatomy, the origins of contractile activity, the control of cardiac growth and size, and the signaling pathways and transcription programs that underpin these processes. Specific chapters are devoted to various muscle and non-muscle cell lineages involved in heart development, including those of the neural crest, endo- and epicardium, fibroblasts, coronary vessels, and cardiac conduction and lymphatic systems.

This collection captures the current state of genetic counseling and examines the changing roles of genetic counselors in the genomic age. The contributors discuss the range of genetics-related needs people bring to counselors, existing and emerging technologies for genetic testing, challenges related to the abundance of recent discoveries about genetic diseases, the ways in which genetic counseling is integrated into modern medicine, and the expanding roles of genetic counselors beyond the clinical setting. The current and evolving practices in different counseling subspecialties, including infertility, prenatal care, pediatrics, cardiology, neurology, psychiatry, and cancer, are also covered.

Influenza is a serious disease that affects millions worldwide every year. This book discusses cutting edge research on the viruses that cause the disease, its effects on the host, and current vaccine design strategies.

Written and edited by experts in the field, this collection provides a comprehensive update on our understanding of the biological basis of and treatment strategies for addiction to psychoactive drugs, with an emphasis on opioids. Contributors examine the molecular targets of alcohol, cannabinoids, nicotine, stimulants, and opioids, the resulting changes to the neurocircuitry, and various genetic, environmental, developmental, and behavioral factors that influence the progression from abuse to addiction and susceptibility to relapse.

Written and edited by experts in the field, this collection from Cold Spring Harbor Perspectives in Biology reviews the arsenal of memory T-cell subtypes in the body and how they function as key components of the adaptive immune system. The contributors examine the heterogeneity and diversity of memory T-cell subtypes, how they are classified and named, and their biological characteristics--their transcriptional and metabolic features, migration patterns, life spans, and surveillance activities. The differentiation of T cells from a naive state, how they establish and maintain a primed memory state, and factors that contribute to their decline are all covered.

Written and edited by experts in the field, this collection from Cold Spring Harbor Perspectives in Biology provides a comprehensive update on all facets of calcium signaling. The contributors discuss the components and activities of the various calcium-sequestering organelles inside cells, the dynamic interactions that lead to spatiotemporally distinct signals in different circumstances, and the downstream effector mechanisms that translate the signals into biological outcomes. Recent advances in the field (e.g., structural insights into the functions of certain calcium channels) are emphasized throughout.

This collection reviews our understanding of the molecular mechanisms involved in establishing and maintaining cell polarity, as well as their relevance for human disorders. Contributors discuss how cells coordinate signaling pathways, membrane trafficking, and cytoskeletal remodeling to ensure proper spatial arrangement of cellular components. Processes such as transcytosis, exocyst-mediated vesicle trafficking, and endosomal sorting are covered in detail. Throughout, the focus is on epithelial cells and how polarity mechanisms facilitate the development of their apical and basolateral surfaces, tube formation, exchange of waste and nutrients, and the generation and maintenance of specialized structures at the cell surface.

Written and edited by experts in the field, this collection covers our current understanding of the molecular underpinnings of leukemia and lymphoma, as well as recent progress in development of therapies that target them. The contributors review the normal functions of hematopoietic stem cells--the precursors to white blood cells--and then examine the genetic, epigenetic, and cellular mechanisms that drive malignant transformation. The roles of clonal hematopoiesis, RNA-binding proteins, and alterations in metabolism and cell signaling (e.g., MAP kinase pathways) are among the topics covered in depth, along with the parts they play in specific types of leukemia and lymphoma.

Written and edited by experts in the field, this collection from Cold Spring Harbor Perspectives in Biology provides a comprehensive update on how the proteostasis network functions in healthy cells and the diseases that result when protein quality control goes awry. The contributors examine the relevant biochemical attributes of proteins (e.g., solubility), the functions of normal protein aggregates (e.g., biofilm formation in bacteria), and the various heat shock proteins, chaperones, translocation machineries, proteasomes, signaling factors, and transcriptional programs involved in proteostasis. The roles of specific subcellular structures-the endoplasmic reticulum, mitochondria, ribosomes, lysosomes, and cytoplasm-in protein quality control are covered, as is the regulation of proteostasis at the organismal level (e.g., via neuronal activity).

Written and edited by experts in the field, this volume covers many aspects of bioelectronic medicine, examining the neuronal pathways that are being targeted for manipulation, the electronic neuromodulation devices that are under development, and how all of this work is leading to new diagnostics and treatment options for patients. The contributors discuss how the stimulation of specific nerves has been successfully used to treat certain conditions and how similar strategies are being investigated as therapeutics for a multitude of gastrointestinal, cardiovascular, respiratory, and renal disorders.

Written and edited by experts in the field, this collection covers the progress that has been made in understanding the molecular pathogenesis of lung cancer and how this information is leading to improved detection and treatment strategies. The contributors review the genomic, transcriptomic, epigenomic, and proteomic changes associated with lung carcinogenesis, the histologic and metabolic features of different types of lung cancer, and the roles of the immune microenvironment and cancer stem cells in tumor maintenance and metastasis. The large databases of demographic and clinical information, high-throughput platforms that generate molecular data, advanced computational methods, and preclinical models that facilitate advances in basic and translational research are also covered.

Written and edited by experts in the field, this collection examines advances in causal inference approaches in human genetics and how they are being used to enhance our understanding of human development and disease. The contributors discuss family-based study designs for causal inference, including twin designs, adoption designs, and in vitro fertilization designs, that separate inherited factors from perinatal environmental exposures. They also review various types of Mendelian randomizationDLa population-based approach that is growing in utility and popularityDLas well as their integration with family-based designs.

The transforming growth factor (TGF-) family is a large group of structurally related proteins that drive developmental programs and control cell behavior. The TGF- family members exert their effects by binding to receptors on the cell surface, activating intracellular signaling pathways that modulate gene expression programs that control normal cell physiology, immune responses, and a variety of developmental processes. TGF- signaling is also important in conditions such as cancer, skeletal disorders, and cardiovascular diseases. Written and edited by experts in the field, this collection from Cold Spring Harbor Perspectives in Biology provides a comprehensive view of the different members of the TGF- family, the signaling pathways they control, and how they influence development, normal physiology, and disease. The contributors describe the various TGF- family ligands, including activins and bone morphogenetic proteins (BMPs), the structures and functions of the type I and type II receptors, and how ligand-receptor binding is regulated. Both Smad and non-Smad signaling pathways are discussed, as is cross talk between TGF- and other signaling pathways (e.g., Wnt). Individual chapters are devoted to the roles of TGF- signaling in specific biological processes, including cell proliferation, mesenchymal differentiation, branching morphogenesis, stem cell biology, immunity, vascular function, reproduction, and neurobiology. The roles of TGF- signaling in cancer, fibrosis, skeletal diseases, and other conditions, as well as prospects for therapeutics, are also covered. The authors also review TGF- signaling in model organisms (e.g., C. elegans and Drosophila), as well as the discovery of TGF- and early research in the field. This volume is therefore a comprehensive reference on the biology of the TGF- family, and it will be valuable for not only cell and developmental biologists but all who wish to learn about this important field of research.

Written and edited by experts in the field, this collection provides a comprehensive update on advances in our understanding of the structure and multiple functions of the ER. The contributors examine how proteins enter the ER, the biogenesis of membrane proteins, and the role of the ER in protein sorting and quality control. In addition, they discuss how lipids are synthesized and transported to and from the ER.

The retina is a layer of neural tissue that lines the inner eye and captures visual stimuli. This volume provides an update on retinal diseases, describing the genetic and pathophysiological basis of the diseases and advances in strategies to treat them.

Bone plays far more than just a structural role in our bodies. It actively communicates with our brains and other organs, releasing and responding to signaling molecules that regulate numerous biological processes. This book reviews the classical functions of bone as well as exciting new work that has revealed novel aspects of its biology.

Multiple sclerosis is an autoimmune disease that affects the brain and spinal cord. The immune system attacks the myelin sheaths that surround and insulate nerve cells, producing a variety of neurological symptoms. This book examines the molecular basis of the condition, the nature of the autoimmune response, and current treatment and vaccine strategies.

Prions are infectious proteins responsible for diseases such as Creutzfeldt-Jakob disease and "mad cow" disease. They are misfolded versions of normal proteins that replicate by converting their normal cellular counterparts into abnormal prion proteins that disrupt cell function and can be transmitted to other cells and individuals. This book examines our under-standing of their structure, biochemistry, and pathophysiology.

Cancer occurs because cells develop mutations that confer a growth advantage. Different subpopulations within the tumor can possess different mutations, and new mutations occur as the cancer progresses. This can allow the tumor to develop resistance to chemotherapy and is a major therapeutic challenge. This book examines this quasi-evolutionary process involved and how work in both molecular and computational biology is shedding light on it.

The ability to grow new tissue and organs is an important goal of regenerative medicine. This book discusses new research in which cells are grown on artificial scaffolds to build replacement tissue that could be used to treat a variety of conditions and produce more organs desperately needed for transplants.

Written and edited by experts in the field, this collection from Cold Spring Harbor Perspectives in Biology focuses on the critical questions currently debated in the field of immune memory and vaccination. Topics include the plasticity of memory CD4 T cells, the formation of germinal centers by memory B cells, the contributions of natural killer cells to immune memory, and the influence of commensal microbiota on immune responses. Several contributors respond to each question, providing a variety of fresh, concise perspectives on each topic.

Written and edited by experts in the field, this collection from Cold Spring Harbor Perspectives in Biology examines the molecular and cellular details of the oscillatory machinery and how they control aspects of our physiology. Contributors discuss the cell-autonomous generation of circadian time and how those mechanisms interact with circuit-level mechanisms in the suprachiasmatic nucleus. Links between circadian and redox systems, as well as the relationship between circadian oscillations and pathogenic infections, are also covered.

Written and edited by experts in the field, this collection from Cold Spring Harbor Perspectives in Biology reviews our understanding of the organization, regulation, and dynamics of cell-cell junctions and the roles they play in morphogenesis, tissue homeostasis, and disease. The contributors examine the assembly and structure of different cell-cell adhesion systems, the plasticity of cell-cell junctions (e.g., during cell migration), and how the junctions act as hubs to sense and transduce various mechanical and chemical signals.

Written and edited by experts in the field, this collection from Cold Spring Harbor Perspectives in Medicine covers the recent progress that has been made in understanding the pathogenesis and treatment of prostate cancer. The contributors review the normal development, anatomy, and histology of the prostate; the genetic, lifestyle, and dietary factors associated with prostate cancer risk; and the alterations in the genome, epigenetic landscape, transcriptional networks, and metabolic and signaling pathways that drive oncogenesis. The role of the tumor microenvironment in prostate cancer pathogenesis is covered, as are the molecular mechanisms of metastasis. The authors also discuss how mouse models and patient-derived tissues can be used to study aspects of the disease and accelerate the development of appropriate therapies.

Written and edited by experts in the field, this collection from Cold Spring Harbor Perspectives in Medicine provides a comprehensive review of the complex molecular machinery that is responsible for cochlear function and describes recent advances in understanding and treating various forms of hearing loss. The contributors focus on the highly specialized cells that are critical for auditory transduction--the hair cells--and their unique molecular and biomechanical characteristics. They also consider the properties of other cochlear components, including the tectorial membrane, synaptic connections, and resident macrophages, as well as the development and evolution of the cochlea as a whole. Topics such as hair cell regeneration, otoacoustic emissions, and ototoxicity are also discussed.

Written and edited by experts in the field, this collection from Cold Spring Harbor Perspectives in Medicine covers the recent progress that has been made in understanding Ras biology, how Ras activation leads to tumorigenesis, and ways in which oncogenic Ras signaling may be targeted therapeutically. The contributors review the biochemical characteristics of the different Ras isoforms (HRAS, KRAS, and NRAS), their main effectors and signaling pathways, and the mutations that lead to their constitutive activation. Recent work showing that some Ras oncoproteins may be effectively inhibited by small molecules is covered, as is work on alternative targets (e.g., enzymes that catalyze posttranslational modifications of Ras) and inhibitors (e.g., nucleic acids).

Written and edited by experts in the field, this collection from Cold Spring Harbor Perspectives in Medicine examines recent progress in our understanding of the biology and pathogenic mechanisms of HAV and HEV, as well as prospects for improving their control. Contributors explore the molecular characteristics and life cycles of HAV and HEV, their interactions with the liver and immune system, the clinical manifestations of the infections they cause, and their transmission via contaminated food and water. Additional topics include the geographic distributions of the various HAV and HEV genotypes, factors that influence their changing epidemiology, and the development of effective vaccines and vaccination strategies.

Written and edited by experts in the field, this collection covers recent insights into how auxin levels are regulated and, in turn, drive various developmental processes in plants. The contributors discuss the biosynthesis, conjugation, and degradation of auxin, the various transporters, receptors, and transcription factors involved in auxin signaling, the interactions of auxin with other plant hormones, and how these are spatiotemporally coordinated. The roles of auxin in specific organs and tissuesDLthe shoot apical meristem, roots, vasculature, and flowersDLare described in detail. The evolutionary history of auxin signaling and its roles in environmental responses (e.g., plantâ€"pathogen interactions) are also reviewed.