Biofysik

Fluorescence is the most popular technique in chemical and biological sensing and this book provides systematic knowledge of basic principles in the design of fluorescence sensing and imaging techniques together with critical analysis of recent developments. Its ultimate sensitivity, high temporal and spatial resolution and versatility enables high resolution imaging within living cells. It develops rapidly in the directions of constructing new molecular recognition units, new fluorescence reporters and in improving sensitivity of response, up to the detection of single molecules. Its application areas range from the control of industrial processes to environmental monitoring and clinical diagnostics. Being a guide for students and young researchers, it also addresses professionals involved in basic and applied research. Making a strong link between education, research and product development, this book discusses prospects for future progress.

The Three Functional States of Proteins explores how structured proteins, intrinsically disordered proteins, and phase separated proteins contribute to the complexity of cellular life, and offers insights into their roles in both health and disease. It discusses the latest research findings and highlight groundbreaking discoveries and innovative methodologies used to study these protein states. Traditionally, the different states of proteins have been defined based on their structures and functions. However, it is becoming increasingly clear that these criteria alone may not be sufficient to capture the complex and multifaceted properties of these molecules. Definitions based on thermodynamics and kinetics are now recognized as potentially more appropriate for comprehensively understanding protein states. Emerging evidence indicates that under physiological conditions, a majority of proteins possess the capability to exist in and transition between the native, droplet, and amyloid states. These distinct states play crucial roles in various cellular functions, influenced significantly by their physicochemical and structural properties. The book also considers the interactions among these states and discusses how their internal organization as individual molecules, as well as their collective organization as molecular assemblies are stabilized. Furthermore, it examines the processes by which these states are formed and the cellular functions associated with each specific state.

Lanthanides in Enzymology and Microbiology, a new volume in the Foundations and Frontiers in Enzymology Series, offers a detailed discussion of lanthanides and lanthanide-dependent enzyme biology. In this book, more than a dozen global experts consider lanthanide enzymology fundamentals, organismal utilization of lanthanides, distribution and diversity of lanthanide-dependent enzymes, regulation of intracellular levels of lanthanides, gene expression regulation via lanthanides, as well as likely applications of lanthanide binding proteins. Lanthanide-dependent methanol and alcohol dehydrogenase metabolism are considered in both methylotrophs and non-methylotrophs, alongside various application areas, from recovery of rare earth elements to developing lanthanide ion binding peptides and biosynthesis of terpolymers through reverse-oxidation pathways. In providing this deep context and pathways for future research, this book acts as an invaluable resource in this emerging field for researchers and students of biochemistry, biotechnology, and environmental microbiology alike.

This book showcases recent trends in brain-computer interface development. It highlights fascinating results in areas such as language decoding, spinal cord stimulation to enable gait and to restore hand functions. The contributions are based on the 12 nominated brain-computer interface projects of the BCI Award 2022. Every year an international jury selects the most innovate BCI projects and nominates 12 projects before selecting the 1st, 2nd and 3rd place winners. In the book, each project is described in detail by the team of scientists behind it, and the editors provide a concluding discussion of the highlights and overall progress in the field.

This book bridges the gap between physical foundations and medical applications of the NMR and MRI technologies, making them accessible to both physicists and biomedical scientists. The physical basis of these technologies is discussed in a manner that can be easily understood by scientists from different backgrounds, aiding them in gaining a clearer understanding of the subject.. For instance, the medical applications of NMR and MRI technologies are described in a way that is accessible to physicists. Moreover, geometrical descriptions and specific mathematical tools are used to facilitate the visualizations of many concepts. Furthermore, the book covers modern technologies such as hyperpolarization and several other state-of-the-art techniques, along with their foundations.

This book describes a new strategy for rehabilitation from injury and/or disease using Crucial Event Therapy. Recent studies have shown that individuals can recuperate more rapidly from surgery and other invasive procedures intended to correct the negative effects of disease or injury through the use of life support systems that operate at the body's natural biofrequencies. The same observation has been clinically shown to reverse the degenerative effects of neurodegenerative diseases such as Parkinson¿s and Alzheimer's Disease.Crucial Event Therapy describes medicine as the operational control of the functions of the human body treated as a network-of-networks, with 1/f-variable crucial events coding the dynamic states of health and disease through information flow within a network and information exchange between biomedical networks. A new way of thinking based on the statistics of Cortical Events is presented and the relevant literature is suitably referenced. This is anideal book for biophysicists and data scientists seeking to understand the connection of complexity measures for the study of consciousness with the clinical aspects of designing a rehabilitation strategy.

This book addresses X-Ray Imaging Systems intended for biomedical engineering technology students and practitioners, and deals with the major technical components of x-ray imaging modalities. These modalities include film-based imaging, digital radiography, and computed tomography. Furthermore, principles and concepts essential to the understanding of how these modalities function will be described. These include fundamental radiation physics, imaging informatics, quality control, and radiation protection considerations.X-Ray Imaging Systems for Biomedical Engineering Technology: An Essential Guide is intended for biomedical engineering technologists, who provide technical advice and services relating to digital radiography and CT departments not only in hospitals but in private facilities as well. Students in radiological technology programs may also find this to be a useful resource.

Sensors & Instrumentation and Aircraft/Aerospace Testing Techniques, Volume 8: Proceedings of the 41st IMAC, A Conference and Exposition on Structural Dynamics, 2023, the eighth volume of ten from the Conference brings together contributions to this important area of research and engineering. The collection presents early findings and case studies on fundamental and applied aspects of Shock & Vibration, Aircraft/Aerospace Testing Techniques including papers on:Alternative Sensing & AcquisitionActive ControlsInstrumentation

This book focuses on biomedical applications of polarized light, covering instrumentation and modeling specific to the field. This will be the first book, written by leading researchers in the field, to tackle this important topic. Readers will learn the fundamentals of polarized light transport and how to develop instrumentation for clinical and preclinical studies. They will also become familiar with the latest advancement in data analysis and image processing for a variety of medical applications. The book is dedicated specifically to the biomedical community, including scientists, engineers, and physicians working on the development of instrumentation for clinical and preclinical use.Emphasizes biomedical imaging and sensing;Describes new computational approaches with examples;Provides detailed descriptions of novel instrumentation.

This book brings together a selection of papers by George Gerstein, representing his long-term endeavor of making neuroscience into a more rigorous science inspired by physics, where he had his roots. Professor Gerstein was many years ahead of the field, consistently striving for quantitative analyses, mechanistic models, and conceptual clarity.  In doing so, he pioneered Computational Neuroscience, many years before the term itself was born.  The overarching goal of George Gerstein's research was to understand the functional organization of neuronal networks in the brain. The editors of this book have compiled a selection of George Gerstein's many seminal contributions to neuroscience--be they experimental, theoretical or computational--into a single, comprehensive volume .The aim is to provide readers with a fresh introduction of these various concepts in the original literature.  The volume is organized  in a series of chapters by subject, ordered in time, each one containing one or more of George Gerstein's papers.

This book covers the tremendous progress in the current understanding of the molecular physiology of voltage-gated calcium channels. This book includes unparalleled insights into structural features of calcium channels due to X-ray crystallography and cryo-EM, which in turn yielded critical information into how these channels function under normal and pathophysiological conditions, and how they interact with calcium channel therapeutics. The chapters investigate how, with the advent of high throughput genome sequencing, numerous mutations in various calcium channel genes have been identified in patients with neurological, cardiovascular, neuropsychiatric and other disorders. This is further complemented through a much larger in vivo toolkit such as knock-out and knock-in mice. The chapters further discuss the increased complexity of calcium channel physiology that arises from mRNA editing and splicing.  Finally, the book also provides an overview of the updated research on calcium channel inhibitors that can be used both in vivo and in vitro, and which may serve as a spring board for new calcium channel therapeutics for human disease. Voltage-Gated Calcium Channels is useful for academic researchers at all levels in neuroscience, biophysics, cell biology and drug discovery.

This book is a broad-based introduction to an increasingly important topic. The overview is easy because each chapter is structured uniformly: theory, methods, realization alternatives, methodical and practical advantages and disadvantages, and examples from industrial technology and medicine as well as research and development practice.Compared to the first edition ¿Biosignal Processing¿, the content of this book has been expanded by a sensor chapter (galvanic and capacitive sensors) and exemplary experimental data. Chapter 7: ¿Stochastic Processes¿ is also new with selected topics such as statistical analysis of time series, signal detection, and signal decomposition. Furthermore, various electronic measuring circuits and calculations have been adapted to the changed legal regulations and standards and some calculation errors have been corrected.The content is oriented to the sequence of the metrological and signal-analytical chain: neuron as a signal source¿sensor technology¿signal amplification and conditioning¿signal sampling and digitization¿methods of biosignal processing¿biostatistics and stochastic processes.The target groupsThe book is suitable for medical technology studies, research, and practice. You can inform yourself compactly across your professional boundaries about the neighbouring fields and topics at the interdisciplinary interface between medicine and technology.

The series Topics in Current Chemistry Collections presents critical reviews from the journal Topics in Current Chemistry organized in topical volumes. The scope of coverage is all areas of chemical science including the interfaces with related disciplines such as biology, medicine and materials science. The goal of each thematic volume is to give the non-specialist reader, whether in academia or industry, a comprehensive insight into an area where new research is emerging which is of interest to a larger scientific audience.Each review within the volume critically surveys one aspect of that topic and places it within the context of the volume as a whole. The most significant developments of the last 5 to 10 years are presented using selected examples to illustrate the principles discussed. The coverage is not intended to be an exhaustive summary of the field or include large quantities of data, but should rather be conceptual, concentrating on the methodological thinking that will allow the non-specialist reader to understand the information presented. Contributions also offer an outlook on potential future developments in the field.Chapters "e;Metal Peptide Conjugates in Cell and Tissue Imaging and Biosensing"e;, "e;Luminescent Metal Complexes as Emerging Tools for Lipid Imaging"e; and "e;Determination and Imaging of Small Biomolecules and Ions Using Ruthenium(II) Complex Based Chemosensors"e; are available open access under a CC BY 4.0 License via link.springer.com.

Unlock the profound mysteries of life at its most fundamental level with "Biophysics: Exploring the Molecular Foundations of Life." Tailored specifically for graduate students in the life sciences, this comprehensive textbook offers an illuminating journey into the captivating realm where physics and biology converge.Delve deep into the heart of biological systems as you embark on a transformative exploration of molecular biophysics. Discover how physical principles underpin the inner workings of living organisms, from the behavior of individual molecules to the dynamics of complex cellular processes.Key Features:Conceptual Clarity: Master the intricacies of biophysical principles through clear and concise explanations.Cutting-Edge Insights: Stay on the forefront of biophysics with the latest research and breakthroughs.Real-Life Relevance: Connect theory to practice with real-world examples and case studies.Visual Learning: Enhance your understanding with engaging illustrations and diagrams.Problem-Solving Prowess: Hone your skills with thought-provoking exercises and problems.Comprehensive Coverage: Explore a wide range of topics, from molecular dynamics to membrane biophysics.Whether you're a biologist seeking to decipher the physical underpinnings of life or a physicist intrigued by the intricacies of biological systems, "Biophysics" is your indispensable guide. Bridging the gap between disciplines, this textbook equips you with the tools to illuminate the complexities of the living world.Prepare to embark on an enriching academic journey that will deepen your appreciation for the profound beauty and elegance of life's molecular machinery. "Biophysics: Exploring the Molecular Foundations of Life" is your passport to unlocking the secrets of existence and advancing your understanding of the life sciences.

This book focuses on energy metabolism and brain functions related to Cortical Spreading Depression of Leao (CSD), an important issue in brain pathophysiology. The first part of the book offers a comprehensive overview of the history and early research on CSD, and then discusses the recent advances in the technology used to map and monitor brain mitochondrial NADH redox state and other physiological functions during CSD. The chapters explore the connection between CSD and mitochondrial function under hypoxia, Ischemia and various drugs treatment, and provide a resource to scientists researching the development of CSD during various brain pathophysiological conditions.  This book is essential to scientists and students working in the field of bioenergetics of the brain and various organs and tissues in the body. The use of this technology is also crucial and applicable in the neuroscience field.

This book provides a selection of recent developments in scanning ion conductance microscopy (SICM) technology and applications. In recent years, SICM has been applied in an ever-increasing number of areas in the bioanalytical sciences. SICM is based on an electrolyte-filled nanopipette with a nanometer-scale opening, over which an electric potential is applied. The induced ion current is measured, which allows to directly or indirectly quantify various physical quantities such as pipette-sample distance, ion concentration, sample elastic modulus among many others. This makes SICM well suited for applications in electrolytes - most prominently for the study of live cells.This book starts with a historic overview starting from the days of the invention of SICM by Paul Hansma at the University of California at Santa Barbara in 1989. SICM is a member of the family of scanning probe microscopies. It is related to another prominent member of the family, atomic force microscopy (AFM), which has found application in almost any field of nanoscale science. The advantages and disadvantages of SICM over AFM are also outlined. One of the most effective and break-through applications of SICM nanopipettes is in electrochemistry. The different routes and applications for doing electrochemistry using nanopipettes are also discussed. In addition the book highlights the ability of SICM for surface positioning with nanometer precision to open up new vistas in patch clamp measurements subcellular structures. Finally the book presents one research area where SICM has been making a lot of contributions, cardiac research and the endeavors to combine SICM with super-resolution optical microscopy for highest-resolution joint topography and functional imaging.

This contributed volume focuses on cardiovascular diseases (CVDs), and explores the ways in which signaling mechanisms at the biochemical, molecular, and cellular levels in the blood vessels (vascular) and heart contribute to the underlying causes of development and progression of the CVDs. This volume covers unique topics such as oxidant signaling in vascular and heart diseases and health, cytoskeletal signaling in vascular health and disease, phospholipase signaling in CVDs, lipid signaling in vascular and myocardial health and diseases, and drug discovery in cellular signaling for cardiovascular diseases.This book assembles the most important discoveries made by leaders on the cellular signaling mechanisms operating behind the development and progression of life-threatening CVDs. It is an extremely useful resource for the investigators in the field of CVDs, and opens the discussion for further discovery of efficient management and effective treatment of the CVDs.

This interdisciplinary book covers the fundamentals of optical whispering gallery mode (WGM) microcavities, light¿matter interaction, and biomolecular structure with a focus on applications in biosensing. Novel biosensors based on the hybridization of WGM microcavities and localized surface plasmon resonances (LSPRs) in metal nanoparticles have emerged as the most sensitive microsystem biodetection technology that boasts single molecule detection capability without the need for amplification and labeling of the analyte. The book provides an ample survey of the physical mechanisms of WGMs and LSPRs for detecting affinity, concentration, size, shape and orientation of biomarkers, while informing the reader about different classes of biomolecules, their optical properties and their importance in label-free clinical diagnostics.This expanded and updated second edition features a new chapter that introduces the reader to advanced in vivo biosensing techniques using WGM microcavities, looking at photothermal sensing, methods for trapping neutral atoms around WGM microcavities, and practical aspects of optoplasmonic sensing. The second Edition now provides a comprehensive introduction to the use of WGM microcavities in physical sensing which includes measurements with frequency combs, macro and micro (one atom) lasers, gyroscopes, optomechanical and parity-time-symmetric sensor devices.Chapter-end problems round out this comprehensive and fundamental textbook, inspiring a host of up-and-coming physicists, bioengineers, and medical professionals to make their own breakthroughs in this blossoming new field. This textbook can be used for both introductory and advanced courses about the modern optics of optical microcavities.

This book provides cutting-edge, up-to-date research findings on the use of bionanocomposites in biodegradable and environmental applications, while also detailing how to achieve bionanocomposites preparation, characteristics, and significant enhancements in physical, chemical, mechanical, thermal properties and applications. This book on biodegradable and environmental properties of bionanocomposites provides a comprehensive and updated review of major innovations in the field of polymer-based bionanocomposites for biodegradable and environmental applications. It covers properties and applications, including the synthesis of polymer-based bionanocomposites from different sources biomaterials-based composites and tactics on the efficacy and major challenges associated with successful scale-up fabrication on bionanocomposites.It is an essential reference for future research in bionanocomposites as topics such as sustainable, biodegradable, and environmental methods for highly innovative and applied materials are current topics of importance. The book covers a wide range of research on bionanocomposite and their biodegradable and environmental applications. Updates on the most relevant polymer-based bionanocomposite and their prodigious potential in the fields of biodegradable and the environment are presented. Leading researchers from industry, academy, government, and private research institutions across the globe contribute to this book. Scientists, engineers, and students with interest in the most important advancements in the field of bionanocomposites involving high-performance bionanocomposites will benefit from this book which is highly application-oriented.   

This is a graduate-level introduction to quantitative concepts and methods in the science of living systems. It relies on a systems approach for understanding the physical principles operating in biology. Physical phenomena are treated at the appropriate spatio-temporal scale and phenomenological equations are used in order to reflect the system of interest. Biological details enter to the degree necessary for understanding specific processes, but in many cases the approach is not reductionist. This is in line with the approach taken by physics to many other complex systems.The book bridges the gap between graduate students¿ general physics courses and research papers published in professional journals. It gives students the foundations needed for independent research in biological physics and for working in collaborations aimed at quantitative biology and biomedical research. Also included are modern mathematical and theoretical physics methods, giving the student a broad knowledge of tools that can shed light on the sophisticated mechanisms brought forth by evolution in biological systems. The content covers many aspects that have been the focus of active research over the past twenty years, reflecting the authors' experience as leading researchers and teachers in this field.