Molekylærbiologi

This book covers the biotechnological advances being used for the understanding of plant diseases and the subsequent enhancement of disease resistance in crop plants. Chapters are focused on recent advances in sequencing technologies, computational resources and genomics tools useful for the identification of loci governing disease resistance. In addition, emphasis is given to novel approaches like genomic selection for achieving significant genetic gain for quantitative disease resistance. The book thoroughly describes sequencing-based approaches like whole genome sequencing, resequencing, and transcriptome profiling being explored for the understanding of disease resistance mechanisms. Finally, several chapters systematically describing the utilities and concerns of high-end technologies like genome-editing are provided. Simplified Illustrations are provided in every chapter to explain different biotechnological approaches and strategies. The book will help to better explore the biotechnological advances in development of disease resistant crop varieties.

This book provides an update on the latest development in the field of microRNAs in cancer research with an emphasis on translational research. Since the early 2000s, microRNAs have been recognized as important and ubiquitous regulators of gene expression. Soon it became evident that their deregulation can cause human diseases including cancer. This book focuses on the emerging opportunities for the application of microRNA research in clinical practice. In this context, computer models are presented that can help to identify novel biomarkers, e.g. in circulating microRNAs, and tools that can help to design microRNA-based therapeutic interventions. Other chapters evaluate the role of microRNAs in immunotherapy, immune responses and drug resistance.Covering key topics on microRNAs in cancer research this book is a valuable resource for both emerging and established microRNA researchers who want to explore the potential of microRNAs as therapeutic targets or co-adjuvants in cancer therapies.

Over the last few decades, various techniques have been developed to alter the properties of plants and animals.  While the targeted transfer of recombinant DNA into crop plants remains a valuable tool to achieve a desirable breeding outcome, integration of transgenes into the host genome has been random, which in part, leads to reduced acceptance of GMOs by the general population in some parts of the world. Likewise, methods of induced mutagenesis, such as TILLING, have the disadvantage that many mutations are induced per plant, which has to be removed again by expensive backcrossing. Advances in genome sequencing have provided more and more information on differences between susceptible and resistant varieties, which can now be directly targeted and modified using CRISPR/Cas9 technology. By selecting specific gRNAs occurrence of off-target modifications are comparatively low. ZFNs and TALENs- based approaches required re-engineering a new set of assembled polypeptides for every new target site for each experiment. The difficulty in cloning and protein engineering prevented these tools from being broadly adopted by the scientific community.  Compared to these technologies, designing the CRISPR toolbox is much simpler and more flexible. CRISPR/Cas9 is versatile, less expensive and highly efficient. It has become the most widely used technology for genome editing in many organisms.Since its inception as a powerful genome-editing tool in late 2012, this breakthrough technology has completely changed how science is performed. The first few chapters in this book introduce the basic concept, design and implementation of CRISPR/Cas9 for different plant systems. They are followed by in-depth discussions on the legal and bio-safety issues accompanying commercialization and patenting of this emerging technology. Lastly, this book covers emerging areas of new tools and potential applications. We believe readers, novice and expert alike, will benefit from this all-in-one resource on genome editing for crop improvement.Chapter 17 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

This book is an elaborate account of the effects of abiotic stressors on cereals crops. It not only discusses the impacts of abiotic stress on the crops but also the physiological, biochemical, and molecular strategies applied in plant of cereal crops to alleviate the detrimental effects of abiotic stressors. The book also elaborates on various molecular response to the abiotic stress. It is a knowledgebase providing readers latest updates on development of high-performance diagnostics, stress induced responses, genomics, phenomics and metabolomics involved in abiotic stress tolerance of cereal food crops. The book is useful for plant scientists and research scholars. Post graduate students of agriculture sciences, plant physiology, botany and biochemistry also benefit from this compilation.

Metabolic programs of individuals are key determinants for disease susceptibility and immune response. This book, edited by experts in the field, summarizes epigenetic signaling pathways that regulate metabolic programs associated with cancer and cancer-related secondary diseases.The first part of the book highlights key metabolic pathways that are implicated in cancer and provides a comprehensive overview on the carbohydrate, protein, lipid, amino- and nucleic acid metabolic pathways that are deregulated in cancer. Special attention is paid to the altered tumor micro-environment that is influenced by the metabolic milieu. Furthermore, the fundamental relationship between the cellular metabolic environment and cell death-mediated autophagy is discussed.The second part of the book covers our understanding of the fundamental epigenetic regulations that are implicated in controlling the metabolic programs in cancer cells. Many aspects of epigenetic regulation of non-coding RNAs as well as DNA/RNA methylation, which influencing metabolic homeostasis in cancer, are discussed in detail. Special emphasis is placed on the epigenetic regulation of the amino acid, glucose/carbohydrate metabolism and epigenetic regulation during hypoxia and its connection to cancer.Last but not least, the third part of the book covers small molecule modulators of histone modifying enzymes, which can be used as therapeutic tools. The readers learn about the cross-talk between epigenetics and immunometabolims, as well as the epigenetic regulation of oncometabolites to combat cancer.  Given its scope, the book will appeal to a broad readership interested in epigenetic, cancer and metabolic research.

This volume provides a comprehensive reference for researchers aiming to bring new techniques and approaches to their scientific research using urodeles. Chapters are authored by leaders in the field and meant to guide readers through laboratory colony husbandry, traditional molecular techniques, experimental manipulation and surgeries, bioinformatics and genomics, transgenics and lineage-tracing, and physiological and organismal techniques. In addition to laboratory methods, this volume highlights techniques developed for field studies and work with wild-caught animals. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and state-of-the-art, Methods in Salamander Research aims to be a practical guide for researchers interested in deploying new  methodologies in their lab or in the field. 

The Advanced Dairy Chemistry series was first published in four volumes in the 1980s (under the title Developments in Dairy Chemistry) and revised in three volumes in the 1990s. The series is the leading reference source on dairy chemistry, providing in-depth coverage of milk proteins, lipids, lactose, water and minor constituents.Advanced Dairy Chemistry Volume 3: Lactose, Water, Salts, and Minor Constituents, Third Edition, reviews the extensive literature on lactose and its significance in milk products. This volume also reviews the literature on milk salts, vitamins, milk flavors and off-flavors and the behaviour of water in dairy products.Most topics covered in the second edition are retained in the current edition, which has been updated and expanded considerably. New chapters cover chemically and enzymatically prepared derivatives of lactose and oligosaccharides indigenous to milk.P.L.H. McSweeney Ph.D. is Associate Professor of Food Chemistry and P.F. Fox Ph.D., D.Sc. is Professor Emeritus of Food Chemistry at University College, Cork, Ireland.

The field of nanotechnology for targeted therapy initiated more than decade ago has grown fast and interest is increasing. Given the importance of the field for targeted drug and gene delivery systems, there are a large number of laboratory investigations today researching nanobiomaterials for diagnostic and therapeutic applications. Because of the ability of scientists to load nanoparticles with any agent, interest continues to grow and technology in this arena is rapidly evolving. These emerging nanobiomaterials-based medicines can overcome the disadvantages of traditional medicines by target-oriented and site-specific delivery of precise medicines (immunotherapeutic agents, chemotherapeutic agents, diagnostic agents, and so on).Pharmaceutical Nanobiotechnology for Targeted Therapy presents an updated overview of recent advancements in the field of pharmaceutical nanobiotechnology and nano-based drug and gene delivery systems. This comprehensive knowledge will allow researchers to discover innovative nanobiomaterials for targeted therapeutics. The chapters deal with various emerging nanobiomaterials for targeted therapeutic delivery systems and the writing is in a style that is easily disseminated and in a manner that can be readily adopted as sources for new and further studies.This book should be useful for researchers and professionals from academia and industry working in the field of nanotechnology, nanobiotechnology, as well as in the field of pharmaceutical nanotechnology. It should also be useful to those interested in a range of disciplines from material science, chemistry, molecular biology, polymer chemistry, and many more interdisciplinary areas.

Epigenetic modifications comprise heritable gene expression changes that occur without alteration of the DNA sequence and 'co-act' with genetic factors to shape development processes and evolutionary trajectories. Multicellular organisms receive different types of environmental stimuli/stresses that trigger epigenetic modifications during development. These environmentally driven mechanisms represent an underlying cause of phenotypic diversity, especially in metazoans. This book aims to present some of the latest epigenetic insights into the development of metazoans (including humans) as an intersection between their ecology and evolution.

With the erratic changes in climate, crop plants are facing many forms of biotic stresses. When plants are under stress, among several gene families, regulatory genes play a vital role in signal transduction in modulating the expression of genes underpinning several defense pathways and targeting regulatory proteins (viz, transcription factors (TFs)) can be the alternative. Transcription factors directly regulate the downstream R genes and are excellent candidates for disease resistance breeding.  Till date, numerous transcription factors have been identified and characterized structurally and functionally. Of them, TF families such as WRKY, NAC, Whirly, Apetala2 (AP2), ethylene responsive elements (ERF) etc. are found to be associated with transcriptional reprogramming of plant defense response. These TFs are responsive to the pathogen's PAMPs/DAMPs - host's PRR protein interactions and specifically binds to the cis-elements of defense genes and regulate their expression. With this background, realizing the importance of TFs in resistance breeding, this book discusses the recent research and developments in this field for various crops.

This book covers various state-of-the-art techniques regarding the associations between transcription factors (TFs) and genes, with a focus on providing methodological and practical references for researchers. The contents cover diverse protocols and summaries of TFs including screening of TF-DNA interactions, detection of open chromatin regions, identification of epigenetic regulations, engineering TFs with genome editing tools, detection of transcriptional activities, computational analysis of TF networks, functions and druggabilities of TFs in biomedical research, and much more. Written for the highly successful Methods in Molecular Biology series, chapters feature the kind of detailed implementation advice from the experts to ensure successful research results. Authoritative and cutting-edge, Transcription Factor Regulatory Networks aims to benefit readers who are interested in using state-of-the-art techniques to study TFs and their myriad effects in cellular life.

Hormones and Reproduction of Vertebrates, Volume 1: Fishes is the first of five second-edition volumes representing a comprehensive and integrated overview of hormones and reproduction in fishes, amphibians, reptiles, birds, and mammals. The book includes coverage of endocrinology, neuroendocrinology, physiology, behavior, and anatomy of fish reproduction. It provides a broad treatment of the roles of pituitary, thyroid, adrenal, and gonadal hormones in all aspects of reproduction, as well as descriptions of major life history events. New to this edition is a concluding assessment of the effect of environmental influences on fishes. Initial chapters in this book broadly examine sex determination, reproductive neuroendocrinology, stress, and hormonal regulation as it relates to testicular and ovarian development and function. Subsequent chapters examine hormones and reproduction of specific taxa, including agnathan, chondrichthyan, and sarcopterygian fishes. The book concludes with an examination of the environmental influences on hormones and reproduction of fishes, including endocrine-disrupting chemicals and climate change. Hormones and Reproduction of Vertebrates, Volume 1: Fishes is designed to provide a readable, coordinated description of reproductive basics in fishes, as well as an introduction to the latest trends in reproductive research and a presentation of our understanding of reproductive events gained over the past decade. It may serve as a stand-alone reference for researchers and practitioners in the field of ichthyology or as one of five coordinated references aligned to provide topical treatment across vertebrate taxa for researchers, practitioners, and students focused on vertebrate endocrinology.

This contributed volume focuses on cardiovascular diseases (CVDs), and explores the ways in which signaling mechanisms at the biochemical, molecular, and cellular levels in the blood vessels (vascular) and heart contribute to the underlying causes of development and progression of the CVDs. This volume covers unique topics such as oxidant signaling in vascular and heart diseases and health, cytoskeletal signaling in vascular health and disease, phospholipase signaling in CVDs, lipid signaling in vascular and myocardial health and diseases, and drug discovery in cellular signaling for cardiovascular diseases.This book assembles the most important discoveries made by leaders on the cellular signaling mechanisms operating behind the development and progression of life-threatening CVDs. It is an extremely useful resource for the investigators in the field of CVDs, and opens the discussion for further discovery of efficient management and effective treatment of the CVDs.

This book covers the latest findings of a wide variety of viral, prokaryotic and eukaryotic macromolecular protein complexes and builds upon the solid macromolecular foundations established by previous volumes of the Subcellular Biochemistry series. Thus, an almost encyclopaedic coverage of the broad field of protein complex structure and function has been established. The 17 interesting chapters included in this book have been organised into four sections: Soluble Protein Complexes, Membrane Protein Complexes, Fibrous Protein Complexes and Viral Protein Complexes. Significant topics present here are: Fatty Acid Synthase, the Fork Protection Complex, Ribonucleotide Reductase, the Kinetochore, G proteins, the FtsEX Complex, the Kainate Receptor, the Photosystem I-antenna, the Mycobacterial Arabinofuranosyltransferases, the the Bacterial Flagellum, the Actomyosin Complex, Motile Cilia, SLS Collagen Polymorphic Structures, and the Reovirus Capsid and Polymerase. Up-dates/expansion of chapter topics present in earlier volumes are now included in chapters here, e.g., those on Ferritin-like proteins and the Multi-tRNA Synthetase. The book is richly illustrated throughout, the result of an impressive integration of structural data from X-ray crystallography and cryo-electron microscopy. The functional aspects of protein-protein interactions are also given a high priority.

This book addresses and dissects the roles and crosstalk mechanisms for the 48 human nuclear receptors (NR) in human health and disease. After a State-of-the-Art introduction by an undisputed and celebrated field leader to provide an overview of the field and its significance, chapters are organized into six sections. The first three sections discuss NR roles in Reproduction & Development, Metabolism and Central Systems. These present to the reader our current understanding of NR signaling in the development and functioning of the reproductive system; the roles in the regulation of energy metabolism; and how NR signaling is more widely integrated into systemic functions from calcium flux to circadian rhythm. The subsequent three sections dissect how aberrant NR functions drive Cancer; how new insights into Genomic Interaction are helping to reveal how NR disruption drives disease; and finally, how Translational Efforts are exploiting this understanding from developing novel NR ligands to establishing how underlying genetic variation impacts NR function. Within these sections the chapters also illustrate emerging understanding of how the epigenome and non-coding genome combine to regulate NR function and impact dysfunction. Increasingly these insights cross-fertilize over cell and disease boundaries and it is unsurprising that NR are being explored in novel and new arenas such as the context of neurological disorders and depression. Thus, there is wide scope for re-purposing of licensed drugs and development of new NR-targeting therapies for a host of conditions and diseases. This unique book brings together many of the leading figures in NR research from across the globe, to discuss emerging roles and their implications for human health and disease. It summarizes the state of the art and shows signposts for future research to further shape this influential field.

Chronobiology and Chronomedicine is an ideal introduction for postgraduates in various fields, reviewing developments and outlining methods to show the depth and breadth of chronobiology, as well as an invaluable companion to researchers with an interest in developing novel therapeutic approaches.

Over the last half century, a quiet revolution has taken place. In a series of breathtaking discoveries, biochemist Thomas Cech and a diverse cast of brilliant scientists have revealed RNA at the centre of biology's greatest mysteries, from how life began to what makes us human to why we age. At last, The Catalyst pulls back the curtain to show how RNA-long sidelined as the passive servant of DNA-defines life from its very origins to our future in the twenty-first century. Recounting his own paradigm-shifting discovery that RNA can catalyse biochemical reactions, as well as his work on the "fountain of youth" telomerase, Cech unfolds how RNA holds the key to the intricate machinery of our cells, the critical processes of ageing and disease and the spectacular powers of breakthrough therapies from CRISPR to mRNA vaccines. From one of our foremost scientists, The Catalyst is a must-read guide to the present and future of biology and medicine.

This detailed volume provides a comprehensive collection of protocols for epigenomic research, powering our ability to analyze epigenetic modifications across the entire genome. Beginning with methods used to investigate epigenomic modifications such as DNA methylation, histone modifications, and chromatin structure, the book continues with methods for manipulating the epigenome, including platforms for epigenome editing, inducible systems for epigenome editing, and epigenetically modified animals. Written for the highly successful Methods in Molecular Biology series, chapters feature introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Epigenomics: Methods and Protocols serves as an ideal resource for researchers looking to further expand the utility and scope of epigenomics research.

The rapid demise of coral reefs worldwide has spurred efforts to develop innovative conservation and restoration methods. Many of these rely on omics approaches to produce genetic, genomic, transcriptomic, epigenomic or metabolomic data to inform conservation and restoration interventions. This book provides the state of play of this field. It discusses topics ranging from how genomic and environmental DNA (eDNA) data can be used to inform marine protected area design and cryopreservation strategies, the use of knowledge on adaptive genetic and epigenetic variation to maximise environmental stress tolerance of coral stock, harnessing transcriptome data to develop early warning markers, the use of microbial symbiont omics data in guiding restoration strategies, to applications of metabolomics and genetic engineering. How best to translate omics data to resource managers is also discussed.

Non-coding RNAs (ncRNAs) are RNA molecules that are not translated into proteins. They are divided into two categories, namely, housekeeping ncRNAs and regulatory ncRNAs. The main function of the ncRNAs is to maintain the normal cell functionalities. Small RNAs, ribosomal RNAs (rRNAs), long ncRNAs and transfer RNAs are some types of non-coding RNAs, which are abundant and functionally significant. Numerous types of small and long non-coding RNAs are considered important regulators of gene expression in a variety of processes, which range from embryonic development to innate immunity. This promotion of gene expression is done by a special ncRNA named enhancer RNA, which is transcribed from the enhancer region of the gene. The human genome is encoded with thousands of small and long RNA transcripts that do not code for proteins. This book explores all the important aspects of regulatory non-coding RNAs. It will prove to be immensely beneficial to students and researchers studying this topic.

Primate Anatomy: Introduction to Extant Primates synthesizes the taxonomy, anatomy, physiology, and genomics of extant primates, including humans. It takes a holistic approach to describing primate skeletal, muscular, and organ structure and function, providing the tools to understanding the fundamentals and state of the science of primatology. Now in its fourth edition, this work introduces its audience to the history and objectives of the field of primatology. It enumerates and profiles extant primates before delving into detailed descriptions of primate skulls, brains, teeth, skeletons, musculature, organs, blood groups, reproduction, and development. In addition, the book highlights recent advances in primate genomics, including new genera and species assignments, and concludes with a glance at the future of the field. Written by longtime expert Friderun Ankel-Simons, this new edition effectively introduces complex biological concepts in a manner accessible to all readers. It will serve as an excellent reference for technical and non-technical audiences, including primatologists, anatomists, paleontologists, anthropologists, conservationists, and naturalists.

Microbial Biostimulants for Plant Growth and Abiotic Stress Amelioration, the latest release in the Biostimulants and Protective Biochemical Agents series, provides readers with insights into the major role of biostimulants in plant growth and development while under abiotic stress. The term biostimulants is broadly used to reference a group of diverse substances and microorganisms that stimulate life or that promote favorable plant responses. They stimulate natural processes to enhance/benefit nutrient uptake, nutrient efficiency, tolerance to abiotic stress, and crop quality. Many biostimulants improve nutrition and they do so regardless of their own nutrient contents. Further, recently microbe-based biostimulants have emerged as important plant protectors under a range of adverse conditions.

With chapter contributions from prominent scientists in the cell wall field, Plant Cell Walls: Research Milestones and Conceptual Insights provides a comprehensive treatment of plant cell wall research accompanied by a historical overview to illustrate how concepts have evolved, and how progress has been enabled by technological advances.