Kemisk biologi

Dette kompendium er målrettet medicin- og tandlægestuderende på 1. semester og har først og fremmest til formål at skabe et overblik over og en forståelse af den kemi, der er grundlaget for senere kurser på medicin- og tandlægestudiet – især biokemi og cellebiologi. Faget kemi er for manges vedkommende forbundet med en generel uforståelighed, og hvis man som studerende har holdt et eller flere sabbatår efter gymnasiet, kan kemi være ekstra udfordrende. Kompendiet indledes derfor med en repetition af vigtige begreber fra gymnasiet. Der er lagt stor vægt på at formidle kemien på en pædagogisk og overskuelig måde, og derfor rummer kompendiet mange illustrationer og oversigter. Desuden er perspektivering af kemien til de kurser, den er grundlag for, vægtet højt. Kompendiet er pensumdækkende for kurset “Cellens Kemiske Komponenter” (CKK). Det indeholder de relevante henvisninger til tabelværket “Kemiske data og oversigter” (kendt som “KDO”), som må medbringes til eksamen. Kompendiets form og indhold er resultatet af mange års erfaring med undervisning af medicin- og tandlægestuderende i kemi, og de studerendes input gennem årene har været af stor betydning.

This third edition provides new and updated chapters detailing preparation of liposomes, physicochemical characterization of liposomes, lipid analysis, drug encapsulation, surface modification, stimuli response as well as cellular interaction, and biodistribution. Also included is an updated chapter on the history and evolution of the field of liposomology. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Liposomes: Methods and Protocols, Third Edition aims to serve as a reference for graduate students, post-doctoral researchers as well as established investigators utilizing lipid-based systems.

This book elucidates the sustainable production of commercially important biomolecules in medicines, food, and beverage processing, through biological systems, including microorganisms, animal cells, plant cells, tissues, enzymes, and in vitro. It discusses promising technologies for the manipulation of cells including, genetic engineering, synthetic biology, genome editing, and metabolic engineering. The initial chapters of the book introduce topics on biomanufacturing, circular economy, strain design and improvement, upstream and downstream processing. The subsequent chapters cover artificial intelligence-assisted production, designer cell factories, biosensors for monitoring biomolecules, different cells factories, biosynthetic pathways, and genome editing approaches for scale-up biomanufacturing. Lastly, the book discusses the opportunities and challenges of implementing biological systems for the production of biomolecules.¿This book is a valuable source for students, researchers, scientists, clinicians, stakeholders, policymakers, and practitioners to understand biomanufacturing for the sustainable production of biomolecules.

This fully updated book presents an account of areas of utility, techniques, and bioinformatic advancements in the field of lipidomics. Beginning with protocols for lipid isolation and extraction, the volume continues with techniques from extractive mass spectrometry to imaging mass spectrometry methods allowing localization of lipids in tissues. These protocols have been complemented by methods addressing specific problems from membranes, fractionated subcellular compartments or organelles to whole organisms. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Lipidomics: Methods and Protocols, Second Edition serves as an ideal guide for biochemists, molecular biologists, neuroscientists, vision research scientists, as well as all biomedical researchers with interest in disease discovery and drug development.

This book is designed to serve as a textbook for core as well as elective courses offered to undergraduate and advanced undergraduate students enrolled in chemistry. This textbook comprehensively deals various topics of organic chemistry such as amino acids, peptides, proteins and enzymes. The text is divided into four chapters: a chapter each dedicated to amino acids, peptides, proteins and enzymes, respectively. The important reactions have been explained with the help of the mechanisms involved. It gives a detailed account of the solution phase and solid phase synthesis of peptides as well as discussing the structure and function of some biologically important peptides. It also covers the classification, nomenclature and mode of action of enzymes, and a detailed account of the structure and function of different co-enzymes. The book also includes pedagogical features like end-of-chapter exercises to aid in self learning. Given the scope, this textbook will be useful for graduate and advanced graduate students pursuing the course of chemistry, especially organic chemistry.

This book provides basic knowledge of the biology, chemistry, and function of oxysterols and its derivatives as well as of phytosterols in numerous human diseases.The book is divided into six sections and begins with an introduction to the biological and chemical properties of oxysterols and its derivatives as well as phytosterols, their synthesis, and the methods currently used for their detection in various biospecimens. The following section discusses in detail the various effects of oxysterols on numerous human diseases, including infectious diseases, inflammatory and autoimmune diseases, atherosclerosis, and cancer, as well as neurological and neuropsychiatric disorders. Importantly, the potential of oxysterols as biomarkers for some of these diseases is also highlighted. The book concludes with an outlook on the pharmacological and nutritional effects of oxysterols and phytosterols and their potential use by the food, and pharmaceutical industries.Aiming to provide an in-depth overview of the biological and the chemical properties of oxysterols and phytosterols and their implications for human health, this book will be of interest to basic and clinical scientists, as well as to anyone working in the food or pharmaceutical industry who is exploring the potential of oxysterols and phytosterols.

This detailed volume explores poly(ADP-ribose) polymerases (PARPs) in the biology of eukaryotes and their relevance to human health. Beginning with a section on the detection and quantification of poly(ADP-ribose) polymer (pADPr), the book continues by delving into the identification of protein targets, functional analysis, the poly(ADP-ribosyl)ating pathway in chromatin and genes expression, as well as the use of animal models and PARP1 inhibitor design and testing, and more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Poly(ADP-Ribose) Polymerase: Methods and Protocols, Third Edition presents essential new and classical methods for studying the pADPr-pathway.

This second edition provides new and updated techniques and applications associated with synthetic biology. Chapters guide readers through the creation and regulation of gene circuits, manipulation of biochemical pathways, genome editing and modification, creating genome language and computing, as well as molecular assembly.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Synthetic Biology: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.

This book aims to advance the understanding of deubiquitinases (DUBs) and DUB-like enzymes. Chapters detail methods used to identify, classify,and biochemically characterize DUBs along with approaches that enable both the determination and alteration of DUB biological function.. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.Authoritative and cutting-edge, Deubiquitinases: Methods and Protocols aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge. 

This volume provides up-to-date and novel techniques for various screening technologies currently used in metagenomics and related areas. Starting with DNA/RNA isolation from environmental samples, the book continues by delving into areas such as current methods used to isolate DNA and construct metagenomic libraries, establishment of metagenome libraries in non-E. coli hosts, and topics like function-driven mining of metagenomic DNA, screening and analyzing protocols for a wide array of different genes encoding enzymes, bacterial viruses and much more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Metagenomics: Methods and Protocols, Third Edition provides a comprehensive collection of up-to-date metagenome protocols and tools for the recovery of many major types of biocatalysts and allows for the easy setup of these screens in microbiology laboratories.

This fully updated edition provides a series of methods for how best to assess functions of histone deacetylases and acetyltransferases. The disease-relevance of dysregulated protein deacetylation by overexpressed or aberrantly activated histone deacetylases has spurred an intense search for novel and improved inhibitors of these enzymes, as reflected in this collection. Expert contributors explore the generation and evaluation of novel histone deacetylase inhibitors and new and improved techniques to assess acetylation-dependent molecular mechanisms in vitro and in vivo. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, HDAC/HAT Function Assessment and Inhibitor Development: Methods and Protocols, Second Edition serves as an ideal guide for researchers seeking to further elucidate this vital area of study.

This detailed volume provides a comprehensive overview of state-of-the-art metabolomics methods based on mass spectrometry (MS), and their application in food, nutrition, and biomedical research. The chapters assembled herein cover hot topics related to sample preparation, chromatographic and electrophoretic separation, MS-based analysis, as well as data processing and analysis. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Mass Spectrometry for Metabolomics serves as a timely guide for chemists, biochemists, biologists, nutritionists, clinicians, and other experts working in the growing and exciting field of metabolomics.

This detailed book examines the main methods to study mammalian monoamine oxidases (MAOs), ranging from cell biology to computational chemistry. Beginning with techniques on how to obtain pure samples of MAO A and MAO B, the volume continues by covering assays and techniques used to measure MAO enzymatic activity and perform inhibition studies, methods to address cellular localization and function of MAOs, either in cell lines or in animal models, as well as computational methods applied to rational drug design approaches that are used to develop new MAO inhibitors. Written for the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Monoamine Oxidase: Methods and Protocols serves as a vital resource for scientists who are interested in studying MAOs and other similar amine oxidase enzymes.

This book covers edge-point applications in science and engineering. The chapters discuss the functional properties of advanced engineering materials and biomolecules, improving the comprehension of their chemical physical properties and potential for new technological and medicinal applications. The book presents a small number of experimental techniques and computational simulation models from basic concepts of classical/quantum mechanics, physics, chemistry, biology, statistical methods that can predict important applications and properties of these materials/biomolecules. The content shows how improving design of new systems helps in addressing future world problems (health, energy, food, environment, transportation, housing, clothing, etc.), i.e., almost every aspects of our daily lives. 

This book presents the applications of systems biology and synthetic biology in cancer medicine. It highlights the use of computational and mathematical models to decipher the complexity of cancer heterogeneity. The book emphasizes the modeling approaches for predicting behavior of cancer cells, tissues in context of drug response, and angiogenesis. It introduces cell-based therapies for the treatment of various cancers and reviews the role of neural networks for drug response prediction. Further, it examines the system biology approaches for the identification of medicinal plants in cancer drug discovery. It explores the opportunities for metabolic engineering in the realm of cancer research towards development of new cancer therapies based on metabolically derived targets. Lastly, it discusses the applications of data mining techniques in cancer research. This book is an excellent guide for oncologists and researchers who are involved in the latest cancer research.

In this Edited Volume, a diverse group of exercise metabolism experts, assembled a multi-facetted collection of fascinating contributions. The chapters focus on metabolism during exercise, including anaerobic and aerobic metabolism, carbohydrate metabolism (separate chapters on muscle glycogen and blood glucose), fat metabolism (separate chapters on muscle and adipose tissue) and protein metabolism. Readers will find discussion on various tissues in addition to skeletal muscle, such as liver, heart and brain metabolism during exercise. In addition, the book includes chapters on other perspectives such as thermodynamic and bioenergetic aspects of exercise and a dive into history. Another focal point is on the effects of exercise in relation to training, age, sex, fatigue and the circadian rhythm.This contemporary collection will be an essential resource for Physiologists, Sports Scientists, Coaches, Athletes and students alike. 

This is the first book to explore the science underlying the Jerusalem artichoke, which is also known as Helianthus tuberosus L. and it is attracting increasing interest among food scientists and professionals worldwide. Due to a wide perspective for the production of inulin due to its high economic and ecological importance, the development of technologies for isolating and processing tuberous sunflower raw materials using environmentally friendly technologies (green chemistry and white biotechnology) and the global production of inulin shows an increasing tendency. Here we focus on the latest technological achievements related to the use of inulin in the food processing. In this book, readers will find full explanation of the conceptual aspects and the latest research results on a wide range of topics, including the relevant characteristics and applications from various fields. Written by leading scientists in the field, the book will be a valuable resource for students and researchers in the fields of food chemistry, nutritional science, physiology, and bioengineers, as well as for professionals in the food industry.

This detailed volume explores protocols for the production of membrane proteins in a panel of heterologous organisms for structural studies. Beginning with techniques using E. coli as a host for the overproduction and purification of membrane proteins, the book continues with chapters covering mammalian membrane protein production in yeast, insect cells, mammalian cells, as well as using virus like particles and acellular systems. Additionally, new detergents and alternatives to detergents allowing membrane protein purification for structural analyses are described. The book closes with a chapter exploring the use of microscale thermophoresis (MST) to evaluate the binding activity of heterologously expressed proteins directly in crude membrane extracts. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Heterologous Expression of Membrane Proteins: Methods and Protocols, Third Edition serves as an ideal guide for scientists aiming to produce and purify functional recombinant membrane proteins for structural studies.

This detailed volume presents a series of methods exploring membrane trafficking research, ranging from genetics and high-resolution imaging to in vitro biochemical and biophysical assays. Covering virtually all the major trafficking branches, the book delves into the exocytic pathway, which focuses on cargo transport from the ER to the Golgi, through the Golgi cisternae, and to the plasma membrane and the extracellular space; the endocytic pathway, which includes cargo endocytosis, endosomal recycling, and lysosomal degradation; as well as emerging topics beyond the conventional exocytic and endocytic pathways. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Membrane Trafficking: Methods and Protocols provides techniques with broad applications as an ideal guide for junior researchers new to membrane trafficking as well as established membrane biologists seeking to expand their research programs.

This book reviews new promising drug targets for Neglected Tropical Diseases (NTDs), with a special focus on antiprotozoal drugs against trpyanosomatids Trypanosoma cruzi and Leishmania spp. The book offers a comprehensive overview of the most recent studied targets, and it outlines classical and new treatments and delivery strategies. Expert contributors describe new methods of analysis and bio-prospecting for new compounds, and provide a critical perspective of the translational process used in the research and development of new drug candidates.The book will appeal not only to researchers, students and professionals interested in drug development to protozoan diseases, but also to medicinal chemists in general.

This book offers a comprehensive review of the latest developments, challenges and trends in C1-based (one-carbon based) bioproduction, and it presents an authoritative account of one-carbon compounds as promising alternative microbial feedstocks. The book starts with a perspective on the future of C1 compounds as alternative feedstocks for microbial growth, and their vital role in the establishment of a sustainable circular carbon economy, followed by several chapters in which expert contributors discuss about the recent strategies and address key challenges regarding one or more C1 feedstocks. The book covers topics such as acetogenic production from C1 feedstocks, aerobic carboxydotrophic bacteria potential in industrial biotechnology, bioconversion of methane to value-added compounds, combination of electrochemistry and biology to convert C1 compounds, and bioprocesses based on C1-mixotrophy. Particular attention is given to the current metabolic engineering, systems biology, and synthetic biology strategies applied in this field.

This volume presents both theoretical guidance and protocols on chemogenomics including chemogenomics library assembly, compound profiling, and phenotypic assays. The chapters in this book cover topics such as the assembly and use of Kinase Chemogenomics; data mining for chemogenomic compound candidates; protocols for protein family-focused assay systems to profile chemogenomic compounds; functional and target engagement assays in cellular settings for broad characterization; and a discussion on phenotypic assays where chemogenomic sets may be applied. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and thorough, Chemogenomics: Methods and Protocols is a valuable resource for all researchers whoare interested in learning more about this diverse and developing field.

This collection explores the latest advances in riboregulators, such as RNA-only systems and ribonucleoprotein systems, and provides detailed techniques to study, evolve, and design them. Beginning with a set of chapters focused on the design and application of small RNA (sRNA) regulator systems, the book continues with sections on techniques to create switchable riboregulator systems known as riboswitches, technologies that leverage RNA-guided CRISPR-Cas systems to edit the epigenome, control gene expression, and create diagnostics, as well as computational and experimental techniques to investigate the sequence-structure-function relationship of RNA systems that can both advance fundamental understanding and rational design of riboregulators. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, andtips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Riboregulator Design and Analysis is an ideal guide for scientists and engineers interested in the design and application of riboregulators and driving further innovation in the field.

This volume details cutting-edge methods and protocols for the development, characterization, and applications of multienzyme assemblies. Chapters guide readers through up-to-date techniques applied for the development and emerging applications of multi-enzymatic systems for biotransformations, biosensing, molecular-scale diagnostics and bioelectronics. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.Authoritative and cutting-edge, Multienzymatic Assemblies: Methods and Protocols aims to be useful for academics and industry professionals working in the field of biotechnology, biochemistry, chemical and biological engineering, nanobiotechnology, and biocatalysis.