Bøger af Guillermo Bracamonte

The development of new Nanomaterials based on hybrid materials is of high interest and impact in several areas of Science due to their large number of applications depending on their composition. In the present book, the design and synthesis of hybrid silica nanocomposites with luminescent properties enhanced by Fluorescence Resonant Energy Transfer (FRET) mechanism and their application to the bio-labeling of cyanobacteria is presented. In particular the study of these Bio-structures is related to a high Clinical and Environmental Interest. Since they possess weak luminescent properties, Energy Transfer processes between Nanoplatforms and Cyanobacteria, such as Bio-FRET mechanisms, were studied. In this way, synthetic luminescent properties were generated and evaluated by Fluorescence, Fluorescence Laser Microscopy and Flow Cytometry with Laser excitation and Fluorescent detection.

In this publication, results obtained and discussed on the fabrication of synthetic Nano-Biostructures have been presented. They have been designed based on Escherichia Coli bacteria and silica-coated metallic core-shell nanoparticles. For this purpose, nanoparticles of reduced dimensions within the nanoscale were synthesized by means of the Turkevich soft reduction method. They were modified with variable lengths of silica spacers (Au@SiO2). On the obtained nanoparticles a fluorescent molecule of the Laser type, such as Fluorescein (Fl), was covalently bonded for the generation of luminescent properties enhanced by the presence of Metal (Metal Enhanced Fluorescence, MEF). In this way the MEF-Au@SiO2-Fl nano-emitters associated with higher intensities and stabilities over time were used as nano-bio-markers of bacterial membranes. From these Nano-Bio-assemblies, new Nano-Bio-optical properties and applications in Life Sciences were evaluated.