Bøger udgivet af Cold Spring Harbor Laboratory Press,U.S.

Wnt proteins are signaling molecules that play critical roles during embryonic development and in the regeneration of adult tissues. They bind to Frizzled and LRP family receptors on the cell surface, triggering a series of events that cause ?-catenin to enter the nucleus and activate transcription factors that control cell fate and cell proliferation. Mutations in components of the Wnt pathway lead to developmental defects and are common in cancer. Written and edited by experts in the field, this collection from Cold Spring Harbor Perspectives in Biology covers all aspects of canonical Wnt signaling, as well as ?-catenin-independent Wnt signaling and cross-talk with other pathways. The contributors examine the numerous Wnt ligands; their production, secretion, and interactions with components of the extracellular environment; and details of the downstream signaling pathways that mediate the effects of Wnt proteins on cells. The roles of Wnt signaling in stem cell self-renewal, cell polarity, body-axis specification, wound healing, and other aspects of normal development and physiology are also covered. This volume includes discussion of Wnt signaling in cancer, skeletal defects, neurological disorders, and other human disease states. Thus, it is an indispensable reference for cell and developmental biologists as well as those, especially in the fields of stem cell biology and regenerative medicine, who are interested in targeting the Wnt pathway for therapeutic purposes.

Based on Sydney Brenner's personal recollections, with contributions and correspondence from his close friends and colleagues, this book tells the lively story, not only of Brenner himself, but of what came to be known as the golden age of biology.

This volume reviews our understanding of two RNA worlds: the primordial RNA world before DNA, in which RNA was both information store and biocatalyst; and the contemporary RNA world, in which mRNA, tRNA, rRNA, siRNA, miRNA, and a host of other RNAs operate.

Based on Cold Spring Harbor Laboratory's long-running course, Drosophila Neurobiology: A Laboratory Manual offers detailed protocols and background material for researchers interested in using Drosophila as an experimental model for investigating the nervous system. This manual covers three approaches to the field: analysis of neural development, recording and imaging activities in the nervous system, and analysis of behavior. Techniques described include molecular, genetic, electrophysiological, imaging, behavioral and developmental methods.

The behavior of lymphocytes in the immune system depends on encounters with antigens. These bind to immunoreceptors on the surface of T-cells and B-cells, activating a variety of signal transduction pathways that control cell survival, proliferation, differentiation, and effector functions. Written and edited by experts in the field, this volume is essential reading for systems biologists as well as all immunologists and cell biologists interested in understanding how lymphocytes function.

A manual containing new, established, and evolving methods for real-time studies of how living cells and organisms work. Appropriate for working scientists from graduate level up to principal investigator.

Experts examine the mechanisms by which cells polarize, divide asymmetrically, and produce asymmetric structures, providing examples from bacteria, yeast, plants, invertebrates, and mammals. Discussion include the molecular basis of polarization, mechanisms, and more.

Many human diseases--including Alzheimer's disease, schizophrenia, cancer, and cardiovascular disease--show complex inheritance that requires sophisticated analysis. Genetics of Complex Human Diseases: A Laboratory Manual brings together the tools that geneticists use to find disease genes with the genetic concepts and statistical theories that underpin these research approaches. Topics covered include basic genetics and Mendelian inheritance, statistical methods, genetic epidemiology, linkage studies, transmission disequilibrium test analysis, variance components analysis, genome-wide association studies, copy-number variation, methods for high-throughput genotyping, the complexity of RNA editing, and genetic computer programs. The book's chapters, written by leading investigators in the field, blend practical information and reviews of each topic, providing both the how and the why of complex disease analysis. Genetics of Complex Human Diseases is an important guide for anyone with an interest in human genetics or who uses genetic techniques in the study of diseases with complex inheritance.

Signaling by diffusible morphogens, such as Hedgehog, Wingless, TGF-, and various growth factors, is vital for developmental patterning, ensuring that distinct differentiated cell types appear in the right place and at the right time in forming tissues. The expert contributors to this volume discuss the roles of morphogen gradients in organisms from yeast to humans. They examine the regulation of morphogen synthesis, trafficking, and diffusion, as well as the complex webs of signaling mechanisms and transcriptional responses in recipient cells. The book is a vital reference for developmental biologists and cell biologists wishing to know how cell fate is determined during embryogenesis.

Comprised of review articles reprinted from the tenth anniversary issue of the journal "Genome Research" that capture the status of genetic research in a collection of model species, this volume serves as a valuable reference and teaching tool for established scientists in the genomics field and as a comprehensive overview for those with more general interests in biology.

This is the story of the sequencing of the fly genome as told by one of the participants, Michael Ashburner. Written in a diary-like form, half the story is told in numerous footnotes. Ashburner has written a delightful, candid, irreverent, on-the-scene tale filled with eccentric personalities all focused on a single goal. The book also contains an Epilogue that puts Drosophila as a model system in historical context, and an Afterword that discusses the impact the genome sequence has had on the study of Drosophila. Also included are portraits by Lewis Miller of some of the principal characters.

This is the first compendium of protocols specifically geared towards genetic variation studies. It includes detailed step-by-step experimental protocols that cover the complete spectrum of genetic variation in humans and model organisms, along with advice on study design and analyzing data.

This is the first book to provide a detailed and thoroughly up-to-date review of the complexity of DNA replication in eukaryotic cells. It is organized into three parts: Concepts, a distillation of underlying principles; Enzymes, a description of each protein class involved; and Systems, a review of events over a wide range of organisms. The book is therefore invaluable for teachers who want a current survey of a topic central to the biology syllabus: investigators of replication who will appreciate a remarkably concise, central source of knowledge in their specialty; and scientists studying other biological functions on which DNA replication has an impact.

For James D. Watson, the year 2003 was momentous: The 50th anniversary of the discovery, with Francis Crick, of the DNA double helix; the 35th anniversary of the publication of his best-selling memoir of the discovery, The Double Helix; the 35th anniversary of his appointment as Director of Cold Spring Harbor Laboratory, an institution he molded into a research and education center of international renown and prestige: and the year in which the sequencing of the human genome was completed, a project of unprecedented international effort and coordination that Watson got off the ground and sustained during its first, critical years. This book gives voice to 43 of these individuals--people of distinction who have worked with Watson as a scientist, educator, author, administrator, and government official. Their essays cover much of his scientific life and, taken together, create a portrait of a complex man whose originality and force of will have produced extraordinary achievements.