Bøger udgivet af Humana Press Inc.

This volume aims to cover all major methodological aspects of research into purinergic signaling and to provide a foundation for studying them at molecular, biochemical, pharmacological, and physiological levels. Chapters guide readers through current knock-out and knock-in mouse models, in silico modeling, knock down purinoceptor expression, bioluminiscence resonance energy transfer, enzyme-based biosensors, recording P2X receptor electrophysiology, controlling P2X receptors by optogenetics, inflammasome activation, leukocyte migration, and cell adhesion. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Purinergic Signaling: Methods and Protocols will provide a sound basis for molecular, cellular, and physiological research into purinergic signaling in health and disease and will spark interest in this fascinating signaling process among researchers in many different and unrelated disciplines.

This detailed volume explores olfactory receptors, a topic that goes beyond classical biochemical essays and involves methods to investigate the brain areas associated with the perception of odorants.

This volume looks into the need for a variety of experimental models for research on traumatic brain injury (TBI) and peripheral nervous system.

Chapters guide the readers through exploiting a number of protein scaffolds including virus nanoparticles, elastin and collagen peptides and proteins, and other protein templates for either building materials or presentation of ligands.

This fully updated volume utilizes the expertise of scientists currently engaged in immunotoxicity testing to provide the reader with lab-ready procedures and the background information needed to identify effective testing approaches.

Chapters guide readers through the production of VNPs derived from plant, animal and bacterial viruses, prokaryotic and eukaryotic expression systems, encapsulation of heterologous materials within VNPs, and the modification of the outer surface of VNPs and how such modified VNPs can be developed into functional entities.

This volume details fast-moving research while providing in-depth descriptions of methods and analytical approaches that are helping to understand the genome and how it is related to complex diseases.

This volume details protocols covering nearly all aspects of fungal genomics. New and updated chapters guide the reader through experimental genomics, biotechnologies, and the analysis and processing of data. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Fungal Genomics : Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.

This book highlights the potential of e-device technology to serve as a successful platform for multiplexed sensing, along with the methods for device fabrication, calibration, and assays in multiple applications.

This volume explores the recent advancements in biomolecular simulations of proteins, small molecules, and nucleic acids, with a primary focus on classical molecular dynamics (MD) simulations at atomistic, coarse-grained, and quantum/ab-initio levels.

This volume aims at providing readers with protocols to enhance their research for the next decade of phytochrome research.

This second edition volume expands on the previous edition with a comprehensive update on recent developments in the Adipose Stromal/Stem Cells (ASC) field and the use of stroma vascular fraction (SVF) as a potential therapeutic strategy. The chapters in this book discuss protocols that are focused on discovery, preclinical, and clinical processes. Although most chapters focus on human ASC, there are several chapters that look at small and large animal species with pre-clinical relevance. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips in troubleshooting and avoiding known pitfalls.Cutting-edge and thorough, Adipose-Derived Stem Cells: Methods and Protocols, Second Edition is a valuable resource for anyone interested in learning more about the scientific advances in the field of ASC biology and application.

Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, tips on troubleshooting and avoiding known pitfalls.

This detailed book explores strategies that have been developed to combat plant virus infection. Beginning with a section on techniques for identifying and studying the virus resistance gene involved in plant innate immunity, the volume continues by delving into techniques related to novel mechanisms of plant virus resistance, methods for the analysis and practical use of RNA silencing, as well as methods for the development of plant viral vaccines. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Antiviral Resistance in Plants: Methods and Protocols serves as an ideal guide for researchers working to combat the serious threats plant virus diseases represent for agricultural production and beyond.

Amino Acid Analysis: Methods and Protocols, Second Edition presents a broad spectrum of all available methods allowing readers to choose the method that best suits their particular laboratory set-up and analytical needs.

This volume explores the diversity in progenitor cell biology methods and uniquely describes techniques for isolating, generating, and characterizing progenitor cells from either tissue or embryonic stem cells.

Beginning with a section covering general procedures for high-throughput protein production, the volume continues with high-throughput protocols adapted to the production of specific protein families, as well as an extensive section on protocols combining high-throughput protein production and their micro-characterization.

This fully-updated book provides systems-based methodologies and innovative technologies that can be used for solving complicated problems of complex systems. A wide range of theoretical and experimental approaches are introduced for practical applications, as the content explores the basic and novel concepts in psychoneuroimmunology (PNI), especially the relationships among stress, inflammation, and psychophysiological disorders, as well as various cutting-edge technologies and methods for PNI studies, including the utilizations of mouse models and vaccine models. In addition, this book focuses on translational medicine by applying PNI methods in clinical practice. As part of the Methods in Molecular Biology series, the chapters seek to provide a practical, state-of-the-art, and holistic view for the translation of PNI into better preventive and personalized medical practice. Authoritative and hands-on, Psychoneuroimmunology: Methods and Protocols, Second Edition will be of use to biomedical students and professionals at all levels who are interested in integrative studies in psychology, psychiatry, neuroscience, immunology, molecular biology, genetics, bioengineering, physiology, pathology, microbiology, systems biology, and clinical medicine.

The chapters in this book are divided into four parts: Part One looks at the entire native antigen and covers traditional structural biology techniques such as nuclear magnetic resonance and x-ray crystallography.

This volume collects a variety of techniques and methodologies developed to facilitate research on integrin biology and to identify ideal targets and approaches for the treatment of multiple organ diseases, with a focus on cancer in particular.

This volume explores and explains how digital PCRs (dPCRs) help in the study of numerous topics, such as infectious diseases, evolution of cancer and treatment responses, somatic mosaicism, genome editing and cell therapy, and food testing for GMOs and pathogens.

This volume focuses on protein analysis, including a wide range of the use of mass spectrometry and other protein methods within neurobiological disciplines. Chapters cover topics such as cerebrospinal fluid (CSF) processing and biobanking; label-free quantitative proteomics; SWATH; top-down proteomics; and experimental strategies based on other ¿omics applied to CSF metabolome, lipidome, and microRNAome. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and thorough, Cerebrospinal Fluid (CSF) Proteomics: Methods and Protocols is a valuable resource for graduate students and post-doctoral fellows interested in learning more about CSF proteotyping. It is also useful to established researchers seeking further insight into this growing field.

This volume covers the latest techniques and strategies used in multi-photon excitation (MPE) microscopy.

This book explores cutting-edge methods to work with the notoriously difficult, but highly prevalent, obligate intracellular pathogen, Chlamydia trachomatis.

This volume provides a variety of methods used to analyze ADAMTS proteases and ADAMTS-like proteins, including their structure, substrate profile, tissue and cell distribution, post-translational modification and biological pathways. Chapters discuss genetic, cell biology, biochemical and proteomics techniques applicable to the analysis of all the members of the ADAMTS superfamily. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, ADAMTS Proteases: Methods and Protocols is a valuable tool for researchers to study both simple and sophisticated methodologies that are often difficult to locate in primary research literature.

This volume explores the rapidly evolving field of HLA typing and its use in both the laboratory setting and in silico methods.

This volume discusses the latest research and techniques used to study novel cellulases. The chapters in this book explore topics such as the role of cellulases in lignocellulose deconstruction for generation of a sugar platform for biofuel; screening for novel fungal b-glucosidases in a variety of fungal strains; discovery of novel enzymes in bacteria or fungi; protocols for isolating cellulolytic rumen bacteria; analysis of enzyme mixtures produced by ligocellulolytic fungi; and homology modeling. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and thorough, Cellulases: Methods and Protocols is a valuable resource for biochemists, molecular biologists, chemical engineers, and industrial researchers interested in this field.

This volume discusses easy-to-follow protocols for researchers in the field of plant chemical genomics. The chapters in this book look at technical workflows of high-throughput chemical compound screens in numerous plant tissues, such as seedlings, roots, and pollen. Chapters also focus on single-celled systems such as Chlamydomonas reinhardtii for chemical screens, and techniques used to modify the target of a small molecule. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Thorough and cutting-edge, Plant Chemical Genomics: Methods and Protocols is a valuable resource to help researchers work with a broad variety of plants for chemical genomics approaches.

This detailed book explores the numerous applications of the yeast two-hybrid (Y2H) system, one of the most commonly used methods to detect protein-protein interactions. Beginning with an overview, the volume then continues by elucidating different methodologies to detect protein-protein interactions in yeast nucleus, membrane, cytoplasm, and bacteria, computational analyses of protein interaction networks, technical variations in yeast one-hybrid (Y1H) systems together with classical and more elaborated yeast methods to detect protein-DNA interactions, as well as protocols to analyze ternary protein interactions and RNA-protein and ligand-protein interactions. The book concludes with a section on methods in plant and mammalian cells that can be used to identify novel protein-protein and protein-DNA interactions and test (qualitatively and/or quantitatively) those observed in yeast. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of detail and key implementation advice that leads to excellent results in the lab. Authoritative and practical, Two-Hybrid Systems: Methods and Protocols serves as an ideal guide to the uses of this exceedingly valuable technique.

This volume provides an introduction for the scientist who is new to the field of molecular imaging, as well as detailed methods for experts in other areas of molecular imaging.