Bøger i Methods in Molecular Biology serien

Following the considerable success of the first edition of Plant Virology Protocols, this exciting new edition covers the many new techniques that are now applied to the examination and understanding of plant viruses.

Amino acid analysis is widely used in biotechnology, biomedical, and food analysis laboratories. Amino Acid Analysis Protocols constitutes a major collection of these indispensable analytical techniques, both classic and cutting-edge, of high utility for answering specific biological questions. Common methods include those based on HPLC or gas chromatography separation and analysis after precolumn derivatization. New techniques based on capillary electrophoresis separation, high-performance anion exchange chromatography, and mass spectrometry are also presented. Since results depend heavily on the quality of the sample, most contributors have devoted a section to sample preparation, particularly to the collection and storage of bodily fluids. A new method for desalting samples prior to hydrolysis is also provided. Each method is described in step-by-step detail to ensure successful experimental results, and contains helpful notes on pitfalls to avoid, and variations that enable the methods to be used with different systems.Up-to-date and highly practical, Amino Acid Analysis Protocols offers analytical and clinical chemists, as well as a broad range of biological and biomedical investigators, a rich compendium of laboratory tools for the productive analysis of both common and uncommon amino acids.

Microelectronic engineering has revolutionized electronics. This book instills in the reader a working understanding of the methods underlying microengineering and the means by which such methods can be used for a range of analytical techniques.

In a single volume this book assembles the various tools and methodologies needed by the interested investigator to unravel lipid dependent signaling and cell function. This is an essential resource for both experienced and novice researchers.

There have been remarkable advances in the study of human pathogenic fungi. In this authoritative book expert researchers explore these exciting new insights, focusing on the study of medically important fungi and Candida spp in particular.

Angiogenesis Protocols, Second Edition remains true to its original vision of providing a single source for angiogenesis researchers, irrespective of levels of resource and expertise, by collecting a range of methods for cell isolation and assessing angiogenesis in vivo or in vitro.

There have been remarkable advances in the study of human pathogenic fungi. In this authoritative book expert researchers explore these exciting new insights, focusing on the study of medically important fungi and Candida spp in particular.

Clinical epidemiology provides the scientific basis for the practice of medicine due to its focus on the diagnosis, prognosis and management of human disease using appropriate research design, measurement and evaluation.

This book sees expert researchers explore current issues and methodologies in this expanding field, addressing areas of gene discovery and functional analysis with a focus on the sub-disciplines of genetic mapping, mRNA, protein and metabolite profiling.

Recent years have seen significant advances in technologies that enable us to analyze transcription factors, a pivotal cell regulatory process in plants. This comprehensive survey covers the basic concepts as well as detailed protocols of tools to study TFs.

In Vitro Neurotoxicology: Methods and Protocols presents a series of cellular, biochemical, and molecular methodological protocols in the area of in vitro neurotoxicology. Features key tips and advice, there is emphasis on mammalian cell culture systems.

This volume reviews the increasing importance of glycosylation to the field of virology, as well as virus replication. The chapters provide an overview of glycosylation in relation to virus infection, and the generic techniques that are used to analyze and characterize glycoproteins.

It is increasingly clear that signal transduction is a highly organized and integrated process. Divided into two convenient sections, this book focuses on experimental approaches to better understand the complexity of signal transduction.

Written in the successful Methods in Molecular Biology (TM) series format, In Vivo NMR Imaging: Methods and Protocols aims to be an experimental compendium of modern in vivo MR imaging with special focus on recent developments in molecular imaging and new protocols for imaging metabolism and molecular markers.

This Methods in Molecular Biology(TM) book shows how PARP proteins act in normal and pathogenic development of organisms, advances knowledge of developmental pathways regulation, and furthers development of new drugs and methods to target PARP-dependent processes.

In recent years, large-scale advances in technology have led to greater understanding of the world at the biomolecular level. In this book, expert researchers from across the globe explore the technology which makes this analysis possible.

Over the last decade, interest in mycoplasmas has been greatly sti- lated by the spread of contagious bovine pleuropneumonia (CBPP), caused by Mycoplasma mycoides subsp.

Despite this setback, advances in recombinant DNA techniques over the last 15-20 years have empowered the engineering of recombinant antibodies with desired characteristics, including properties to avoid HAMA.

It is now more than ten years since Dr. Alec Jeffreys (now Professor Sir Alec Jeffreys, FRS) reported in Nature that the investigation of certain minisatellite regions in the human genome could produce what he termed DNA fingerprints and provide useful information in the fields of paternity testing and forensic analysis.

Putrescine and spermidine are ubiquitous in living organisms. Spermine, third of the three most commonly occurring natural polyamines, is probably present in all eukaryotes but is rare (or nonexistent) in prokaryotes. Polyamine residues are constituents of many compounds found in plants and insects. Putrescine, spermidine, or spermine-containing alkaloids are found in many plants, nonproteinaceous spider and wasp toxins contain polyamine residues, and glutathionyl¿spermidine conjugates have been found in some pathogenic microorganisms. In most cells polyamines are the products of a highly regulated bios- thetic pathway. It is not clear whether the elaborate regulation of polyamine synthesis is a consequence of their essential role(s) in cellular differentiation and development, or part of a defense mechanism to prevent overaccumulation of compounds that are toxic in excess. In addition to their biosynthetic capa­ bility, many cells also possess transport systems for polyamines that respond to intracellular polyamine levels, and other stimuli, and are regulated by mecha­ nisms that are at present incompletely defined. Two routes of polyamine catabolism have been identified in mammalian cells, a biodegradative route and a recycling pathway. The relative impor­ tance of these pathways and their overall regulation is only partially resolved. What is clear is the widespread occurrence of a variety of polyamine-oxid- ing enzymes in animals, plants, bacteria, and fungi. Polyamine catabolism, by whichever route, results in the formation of aminoaldehydes as intermediates.

General initiation factors are involved in positioning RNA polymerase II on the core promoter, but the complex - teraction of these proteins and transcriptional activators binding to DNA e- ments outside the core promoter regulate the rate of transcriptional initiation.

Neurodegeneration tackles the challenges involved with neurodegenerative disorders, such as Alzheimer's and Parkinson's disease. The text presents cutting-edge analytical and technological approaches to study the mechanisms underlying neurodegenerative processes.

Protein Expression in Mammalian Cells presents a compendium of vital techniques to further our knowledge of mammalian protein expression. Each chapter includes a brief introduction, step-by-step, readily reproducible laboratory protocols, and troubleshooting tips.

Increasing interest in mitochondrial bioenergetics is being driven by the impact of drug and environmental chemical-induced disturbances of mitochondrial function as well as hereditary deficiencies and the progressive deterioration of bioenergetic performance with age.

At the heart of most high-throughput methods is the technique of polymerase chain reaction (PCR). With intricate descriptions of basic approaches as well as specialized methods, "PCR Primer Design" is an exceptional reference for all those involved in studying the genome.

Evaluating potential mutagenic activity is a critical step in assessing the safety of both new and pre-existing chemical types. In this book, expert contributors help to satisfy the demand for education in this vitally important field of research.

Knowledge of the three-dimensional structure of a protein is absolutely required for the complete understanding of its function. Historically, structural determination of proteins was limited to those expressed naturally in large amounts, or derived from a tissue or cell source inexpensive enough to warrant the use of large quantities of cells.

It is our hope that Genomics, Proteomics, and Clinical Bacteriology: Methods and Reviews will play a part in bringing such a development to fruition. These topics, and others, are considered in Genomics, Proteomics, and Clinical Bacteriology: Methods and Reviews.

This selection of protocols and procedures includes the latest techniques and shows how to make use of cellular, tissue, and whole animal models which can then be applied to the investigation of neurotrophic factors and other agents impacting on these systems.

Ribozymes details protocols and techniques commonly used to study individual ribozymes and their characterizations. Chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and troubleshooting tips.