Regenerativ medicin

This contributed volume gathers the latest knowledge in the field of stem cells in human reproductive organs, as well as animal models, and to consider the possibility of using this knowledge for clinical purposes. The scope of the book covers both clinical and basic knowledge of stem cells in both reproductive medicine (gynecology and obstetrics) and regenerative medicine as well as cellular and molecular medicine and reproductive biology. Chapters on basic stem cell knowledge in human reproductive tissues and organs or animal models are included, as well as clinical knowledge on their role in the manifestation of infertility and cancer and their clinical use.

This book presents a systematic overview of the technologies currently being explored and utilized in the fields of cardiovascular tissue engineering and regenerative medicine. Considering the unprecedented rapid progress occurring on multiple technological fronts in cardiac tissue engineering, this important new volume fills a need for an up-to-date, comprehensive text on emerging advanced biological and engineering tools. The book is an important resource for anyone looking to understand the emerging topics that have the potential to substantially influence the future of the field. Coverage includes iPS stem cell technologies, nanotechnologies and nanomedicine, advanced biomanufacturing, 3D culture systems, 3D organoid systems, genetic approaches to cardiovascular tissue engineering, and organ on a chip. This book will be a valuable guide for research scientists, students, and clinical researchers in the fields of cardiovascular biology, medicine, and bioengineering, as well as industry-based practitioners working in biomaterial science, nanomaterials and technology, and rapid prototyping and biomanufacturing (3D bioprinting). 

This handbook focuses on different aspects of anti-aging and both preventive and regenerative medicine. It includes analysis of the paradigm of ageing and concepts of anti-aging medicine.Standards and technologies are highlighted in over twenty chapters all authored by international experts in regenerative medicine.Topics covered include:¿ Ageing, aging, and anti-aging: A Decalogue for anti-aging medicine¿ Lessons from Sicilian centenarians for anti-aging medicine¿ Molecular biomarkers and genetic aspects of aging¿ Future of peptides in clinical practice¿ Mitochondrial approaches in anti-aging medicine and in SARS-CoV2 infection¿ Tissue-specific autoantibodies in preventive medicine¿ Chronic stress as a psycho-neuro-immunological dysfunction¿ Gut-associated immune system and its health implications¿ Regenerative medicine with platelet-rich-plasma¿ Alzheimer's disease: Preventive and anti-aging neurology¿ Autistic spectrum disorder and mitochondrial medicine¿ Integrative hormonal approach in anti-aging medicine¿ Preventive cardiology and phlebology¿ Aesthetic and anti-aging medicine¿ Preventive ophthalmology¿ Preventive oncology¿ Nutrition in healthy aging¿ Physical activity and fitness paradigms for anti-aging and longevityRecommended reading for clinicians working in anti-aging medicine as well as ageing people. The authors hope it will set a new "standard of anti-aging medicine" and as a tool in planning for the inevitable challenges we all will face caring for ageing patients and creating preventive-health programs.

This volume details protocols on animal cloning by Somatic cell nuclear transfer for basic research and biotechnological applications. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and methods, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Somatic Cell Nuclear Transfer Technology aims to be comprehensive guide for researchers.

This book reports on fundamental research, cutting-edge technologies and industrially-relevant applications in biomedical engineering.  It covers methods for analysis, modeling and simulation of biological systems, reporting on the development and design of advanced biosensors, nanoparticles and wearable devices. It covers applications in disease monitoring and therapy, tissue engineering, sport and rehabilitation, and telehealth. It also reports on engineering methods for improving  and monitoring medical service, and on advanced robotic applications. Gathering the proceedings of the XLV Congreso Nacional de Ingenieria Biomedica (CNIB2022), organised by the Mexican Society of Biomedical Engineering, this book offers a timely snapshot on technologies and methods in bioengineering, and on challenges related to their practical implementation in the health sector. 

This book presents a fundamental basic overview of orthopedic biomechanics in sports medicine, with a special focus on the current methodologies used in modeling human joints, ligaments, and muscle forces. The first part discusses the principles and materials, including the use of finite element analysis (FEA) to analyze the stress-strain response in the implant-bone interface and design.  The second part focuses on joint-specific biomechanics, highlighting the biomechanics of the knee and shoulder joints, their modeling, surgical techniques, and the clinical assessment of joint performance under various kinematic conditions resulting from different repair techniques. Written by international experts working at the cutting edge of their fields, this book is an easy-to-read guide to the fundamentals of biomechanics. It also offers a source of reference for readers wanting to explore new research topics, and is a valuable tool for orthopedic surgeons, residents, and medical students with an interest in orthopedic biomechanics.

This volume explores the latest techniques used to study the field of tissue engineered vascular grafts (TEVGs). The chapters in this book cover a wide array of topics such as deriving vascular cells from monocytes and induced pluripotent stem cells; engineering vascular grafts using various biomaterials and stem cells, stem cell-derived, or primary vascular cells; biomaterial modification by anticoagulation molecules; vascular bioengineering technologies such as 3D bioprinting; and fabrication of TEVGs with different geometry and multiphase structures. This book also features protocols for grafting and evaluation of vascular grafts in animal models, vascular imaging in animals, and the quantification of blood vessel permeability. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tipson troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Vascular Tissue Engineering: Methods and Protocols is a valuable resource for biomedical engineers, cell biologists, vascular surgeons, doctors, and nurses.

This contributed volume applies the insights of supramolecular chemistry to biomedical applications such as ions/water transport through nano-scale channels, gene therapy, tissue engineering and drug delivery, to cite some of the major investigations.The challenge is to understand the mechanisms of transport through tissues particularly in the therapeutic treatment of a disease where the active drug must be delivered directly to diseased cells without affecting healthy cells. As a result, smaller quantities of active substances can be used to treat the disease. Another interest concerns new ways to administer gene therapy. If genes are often delivered to their target cells by adapted viruses, the supramolecular non-viral ¿vectors¿ using dynamic nano-frameworks and nano-structures are presented. In addition, it is important to reconstruct damaged tissues by mimicking natural processes in cells and polymers, such as tissue engineering and self-healing. Differentoptions are here discussed: e.g. hydrogels based on chitosan, a carbohydrate polymer, are proving especially promising for tissue engineering and drug delivery. For controlled delivery of drugs or other biologically active compounds, hydrogels sensitive to the most important stimuli in the human body, such as temperature, pH, ionic strength, glucose and biomolecules released by the organism in pathological conditions have been developed. Finally, to assist and validate the experimental studies, computer modelling and simulations of large-sized molecular structures and systems using different molecular dynamics and quantum mechanical techniques are developed based on the experimental and chemistry synthesis.This book is of great interest for graduate students, researchers and health professionals interested in acquiring a better understanding of the mechanisms of medical treatments. In addition, it provides numerous tools to develop better therapies for human diseases.

This detailed book explores the fundamentals of myofibroblast biology in tissue repair, fibrosis, and tumors as well as providing cutting-edge laboratory methods used to investigate myofibroblast functions in physiological and pathological settings in vitro and in vivo, as written by leading academic scientists. Section I of this volume focuses on fundamental methods to study myofibroblast biology and covers topics such as methods for detecting myofibroblasts and senescent myofibroblasts in cell culture and histology, single cell RNA sequencing to identify myofibroblast subsets in fibrotic tissues, and functional assays to assess TGF-¿ activation, myofibroblast apoptosis, or matrix deposition and crosslinking. Section II discusses methods to investigate the mechanobiology of myofibroblasts in vitro, including the fabrication of functional hydrogels with tunable stiffness, the use of atomic force microscopy to characterize matrix and cellular stiffness, as well as molecular assays to assess fibroblast mechanotransduction pathways and durotaxis. Section III describes multiple animal models to investigate myofibroblast functions across organs in vivo as well as human organoid systems, precision tissue slices and decellularized 3D tissue scaffolds to assess myofibroblast functions in relevant human ex vivo models. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and extensive, Myofibroblasts: Methods and Protocols is an essential collection for researchers delving into the processes and effects of these important cells.