Videnskabeligt udstyr, forsøg og teknikker

This book covers all the technical aspects of flow cytometry needed to set-up the instrument, solve problems encountered in daily work, or necessary for exam preparation. It provides the reader with an in-depth look at the device and its applications.Each component and its function is described in an easy-to-understand manner, giving the reader a sound basic knowledge of this instrument. The practical examples given, simplify and enhance the learning process.This book is a unique resource of knowledge for biomedical engineers and biotechnologists, flow cytometry operators, laboratory technicians and biomedical researchers, both biologists as well as medical doctors, and can also be a helpful tool for companies and manufacturers.

This detailed volume focuses on genotyping and validation in addition to information on how to produce gene edited cells and animals for research. Future advances in biomedical research will benefit greatly from the use of precise gene targeting of transgenes in the genome as CRISPR technology supersedes earlier methods that relied on random transgene integration, which this collection reflects. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips for troubleshooting and avoiding known pitfalls. Authoritative and practical, Transgenesis: Methods and Protocols serves as an ideal guide for researchers working with or working on genetically modified models.

This laboratory manual includes the latest tools and techniques involved in genomic research. It starts with an introductory chapter on genomics and the various tools and applications involved. The initial chapters present protocols for basic techniques such as DNA isolation, electrophoresis, PCR, cDNA synthesis etc. The book then goes on to describe more advanced techniques such as next-generation sequencing, exome sequencing, use of RNAi, RNAseq, genome editing, single cell genomics etc. Each topic includes a brief description, information on the principles involved, materials & methods, protocol, and expected results, with diagrams and graphs. All protocols are presented in a very lucid and precise way, to make it easy for readers to follow and replicate them.

This book reviews recent physicochemical and biophysical techniques applied in drug discovery research, and it outlines the latest advances in computational drug design. Divided into 10 chapters, the book discusses about the role of structural biology in drug discovery, and offers useful application cases of several biophysical and computational methods, including time-resolved fluorometry (TRF) with Forster resonance energy transfer (FRET), X-Ray crystallography, nuclear magnetic resonance spectroscopy, mass spectroscopy, generative machine learning for inverse molecular design, quantum mechanics/molecular mechanics (QM/MM,ONIOM) and quantum molecular dynamics (QMT) methods. Particular attention is given to computational search techniques applied to peptide vaccines using novel mathematical descriptors and structure and ligand-based virtual screening techniques in drug discovery research. Given its scope, the book is a valuable resource for students, researchers and professionals from pharmaceutical industry interested in drug design and discovery.

Discover the sense of accomplishment after watching your rockets, fireworks, and launchers soar into the sky! Originating from Instructables, a popular project-based community made up of all sorts of characters with wacky hobbies and a desire to pass on their wisdom to others, Do-It-Yourself Backyard Rockets is made up of projects from a medley of authors who have collected and shared a treasure trove of rocket-launching plans and the knowledge to make their projects soar!Do-It-Yourself Backyard Rockets gives step-by-step instructions, with pictures to guide the way, on how to launch your very own project into the sky. All of these authors have labored over their endeavors to pass on their knowledge and make it easier for others to attempt. Discover how to create the following projects: Teeny, Tiny Rocket Engine Ultimate Straw Rocket Rocket Eggstronaut Pocket Rocket Launcher Iron Man Model Rocket Model Rocket with Camera Rocket-Powered Matchbox Cars – Extreme And much more! The Instructables community has provided a compendium of rocket savvy from innovators who have paved the way for other curious minds of all ages!

This book serves as a brief introduction to phylogenetic trees and molecular evolution for biologists and biology students. It does so by presenting the main concepts in a variety of ways: first visually, then in a history, next in a dice game, and finally in simple equations. The content is primarily designed to introduce upper-level undergraduate and graduate students of biology to phylogenetic tree reconstruction and the underlying models of molecular evolution.  A unique feature also of interest to experienced researchers is the emphasis on simple ways to quantify the uncertainty in the results more fully than is possible with standard methods.