Biofysik

Biocatalysis in Asymmetric Synthesis, a new volume in the Foundations and Frontiers of Enzymology series, offers an applied discussion on synthesizing biological catalysts using asymmetric synthesis for varying applications. Here, global experts in the field analyze a wide variety of biocatalysts and their physical states, process conditions for their asymmetric synthesis, solvents required during synthesis, and even downstream procedures for the recovery of final products. The book adopts an interdisciplinary approach, merging fundamental biology and its synthetic applications across industries with a wide range of practical examples, from directed evolution to biotransformation and production of novel enzymes and non-conventional catalysts. Throughout the book, the impact and application of biocatalysis in sustainable processing is considered in-depth. This book will also help non-experts in biocatalysis to apply this knowledge in their own research, providing a thorough overview of the ways asymmetric biocatalytic approaches may be adapted for different disciplines and downstream products.

The Enzymes, Volume 54 highlights new advances in enzymes, with new chapters on a variety of topics, including the History of The Enzymes, Impact of The Enzymes in chronicling biochemical processes and pathways, Metabolism and Catalysis, Mitochondrial ATP synthase, The respiratory chain, A century of mitochondrial research, Five decades of metalloenzymology, Mechanisms of catalysis, Mitochondrial fatty acid synthesis and associated processes, Signaling, MAPK cascades: Origins, mechanisms and current status, Sphingolipids: From structural components to signaling hubs, Protein Homeostasis and Hydrolysis, Mitochondrial AAA+ proteases, Hsp70 and JDP proteins: structure-function perspective on molecular chaperone activity. Other sections cover DNA Replication and Repair, Structure-function studies of DNA replication proteins, and Helicases required for nucleotide excision repair.

Lipases: Foundations, Applications, and Perspectives, a new volume in the Foundations and Frontiers of Enzymology series, offers a practical, in-depth discussion of lipases and instruction in their use across research and industry. Here, global experts in the field introduce readers to basic lipase biology and the history of the field, interfacial activation and lipase lids, evolving analytical techniques and technology for studying lipases, and a range of lipase applications, from health and drug discovery to biofuels, food, cosmetics, and waste treatment. Lipase sources and production methods discussed include mammalian lipases, microbial lipase production, biomass lipase production, immobilized lipases, and tailor-made recombinant lipases, among other approaches.

This book presents a novel method for the numerical simulation of swimming animals. It includes a review of the hydrodynamics of swimming, a description of the CFD model adopted, and a description of the results obtained by applying this model to the cownose ray. This method is developed for the open-source software OpenFOAM and relies on an overset mesh. A custom library is added to the solver to include the equations of the kinematics of the animal under investigation, combining the deformation of the fish fins with the computed displacement and rotation of the animal's body. The presented method helps investigate the dynamics of any animal moving in a fluid, provided that its kinematics is known, and in this work, it is applied to investigate the hydrodynamics of a cownose ray. This book is intended for researchers and engineers who aim to deeply understand the hydrodynamics of fish swimming and to design bioinspired autonomous underwater vehicles or novel propulsion systems.

This protocol book presents reproducible and step-by-step procedures for the peptide synthesis, their characterization and applications. The volume includes an introductory section on in silico modelling of new peptide molecules, Molecular Dynamics Simulations, Docking, Electrostatic fingerprinting of peptides, and other modelling tools for peptide designing and optimization. Further, it covers protocols for the solid phase peptide synthesis, chromatographic and mass spectrometric characterization of peptides. Importantly, it covers methods for biophysical characterizations of peptides for their potential applications as drug delivery vehicles, peptide nano-assembly, bionanocatalysis, protein aggregation diseases, and peptide-based anti-bacterial.¿

How does consciousness emerge from a brain that consists only of physical matter and electrical / chemical reactions? The deep mysteries of consciousness have plagued philosophers and scientists for thousands of years. This book approaches the problem through scientific studies that shed light on the neural mechanism of consciousness, and furthermore, delves into the possibility of artificial consciousness, a phenomenon that may ultimately solve the mystery. Finally, two key suggestions made in the book, namely, a method to test machine consciousness and a theory hypothesizing that consciousness emerges from a neural algorithm, reveal a novel and credible pathway to mind-uploading.The original Japanese version of this book has become a best-seller in popular neuroscience and has even led to a neurotech startup for mind-uploading.

This book covers latest information on organic and inorganic waste management, and how the waste can be utilized as an energy source. An increasing world population and climate change rate hint that environmental health needs a sustainable waste recycling system worldwide. Management of wastes material plays a substantial role in the environment and climate regulation. Chapters contain modern tools and techniques for managing inorganic, biomedical, municipality, and food waste. The title covers the role of contemporary microbiology and biotechnological tools in waste management and how these microbial agents can enhance waste degradation and bioenergy production. The book covers interesting topics such as bio-ethanol production from agro-waste, microbial fuel cells, biogas production from animal waste, nanotechnology in waste recycling, etc. The primary audiences are researchers, scientists, students, and policymakers interlinked with waste management and applied microbial sectors. ¿

This third edition volume expands on the previous editions with new discussions on the latest techniques and developments in the field. The chapters in this book are organized into four parts, and cover topics such as optical tweezers; single-molecule fluorescence tools; atomic force microscopy; magnetic tweezers; applications to virus protein shells, unfolding of proteins, nucleic acids, motor proteins, in vivo and in vitro; and protocols to establish specific surface interactions and perform force calibration. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and thorough, Single Molecule Analysis: Methods and Protocols, Third Edition is a valuable resource for all researchers who wantto learn more about this exciting and still expanding field.Chapters 2, 7, 8, 9, 12, 18, and 19 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

This book presents a collection of chapters on modern bioelectrochemistry focusing on new materials for biodevice, bioelectrosynthesis and bioenergy. The chapters cover protein engineering, semiconductors, biorecognition, graphene-based bioelectronics, bioelectrosynthesis, biofuel cells, bioinspired batteries and biophotovoltaics.

Providing a comprehensive overview of advanced biophysical methods for the characterization of molecular chaperones, most of the contributions are NMR methodology targeted at both current practitioners of structural biology and scientists who are interested in entering the field.

This book provides the reader with an updated comprehensive view of the rapidly developing and fascinating field of fluorescence spectroscopy and microscopy. In recent years, fluorescence spectroscopy and microscopy have experienced rapid technological development, which has enabled the detection and monitoring of single molecules with high spatial and temporal resolution. Thanks to these developments, fluorescence has become an even more popular method in physical, biological and related fields. This book guides the reader through both basic and advanced fluorescence spectroscopy and microscopy approaches with a focus on their applications in membrane and protein biophysics. Each of the four parts: A - Fluorescence Spectroscopy, B - Fluorescence Microscopy, C - Applications of Fluorescence Spectroscopy and Microscopy to biological membranes and D - Applications of Fluorescence Spectroscopy to protein studies are written by experts within the field. The book isintended for both complete beginners who want to quickly orient themselves in the large number of existing fluorescent methods, as well as for advanced readers who are interested in particular methods and their proper use.Chapter ¿Dynamics and Hydration of Proteins Viewed by Fluorescence Methods: Investigations for Protein Engineering and Synthetic Biology¿ is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

This book presents the latest findings in the field of cardiac mechanobiology in health and disease.Cardiac mechanobiology provides knowledge of all aspects of mechanobiology of the heart. Cardiomyogenesis is discussed as well as the mechanobiology of cardiac remodeling and regeneration. The molecular mechanisms of mechanoperception and mechanotransduction in cardiomyocytes are explained, as well as stretch induced differentiation of cardiomyocytes derived from induced pluripotent stem cells. This volume of the series Cardiac and Vascular Biology complements the volume Vascular Mechanobiology in Physiology and Disease (volume 8) published in this series. The book is aimed at clinicians as well as researchers in cardiovascular biology, bioengineering and biophysics, and also represents an educational resource for young researchers and students in these fields.

This book comprises select papers presented at the conference on Technology Innovation in Mechanical Engineering (TIME-2021). The book discusses the latest innovation and advanced research in the diverse field of Mechanical Engineering such as materials, manufacturing processes, evaluation of materials properties for the application in automotive, aerospace, marine, locomotive and energy sectors. The topics covered include  advanced metal forming, Energy Efficient systems, Material Characterization, Advanced metal forming, bending, welding & casting techniques, Composite and Polymer Manufacturing, Intermetallics, Future generation materials, Laser Based Manufacturing, High-Energy Beam Processing, Nano materials, Smart Material, Super Alloys, Powder Metallurgy and Ceramic Forming, Aerodynamics, Biological Heat & Mass Transfer, Combustion & Propulsion, Cryogenics, Fire Dynamics, Refrigeration & Air Conditioning, Sensors and Transducers, Turbulent Flows, Reactive Flows, Numerical Heat Transfer, Phase Change Materials, Micro- and Nano-scale Transport, Multi-phase Flows, Nuclear & Space Applications, Flexible Manufacturing Technology & System, Non-Traditional Machining processes, Structural Strength and Robustness, Vibration, Noise Analysis and Control, Tribology. In addition, it discusses industrial applications and cover theoretical and analytical methods, numerical simulations and experimental techniques in the area of Mechanical Engineering. The book will be helpful for academics, including graduate students and researchers, as well as professionals interested in interdisciplinary topics in the areas of materials, manufacturing, and energy sectors.

This book illustrates the role of randomness and noise in living organisms. Traditionally, the randomness and noise have been used in understanding signal processing in communications. This book is divided into two sections, the first of which introduces readers to the various types and sources of noise and the constructive role of noise in non-linear dynamics. It also analyses the importance of randomness and noise in a variety of science and engineering applications. In turn, the second section discusses in detail the functional role of noise in biological processes for example, in case of brain function at the level of ion channel, synaptic level and even at cognitive level. These are described in various chapters. One of the challenging issue finding the neuronal correlates of various meditative states is to understand how brain controls various types of noise so as to reach a state of synchronized oscillatory state of the brain corresponding to the state of Samadhi. This is described in details in one chapter called Noise, Coherence and meditation. The concept of noise and the role of randomness in living organism raise lot of controversy for last few decades. This is discussed in a separate chapter. Finally, the epistemic and ontic nature of randomness as discussed in physical science are investigated in the context of living organism.

Engineering and Physical Approaches to Cancer addresses the newest research at this interface between cancer biology and the physical sciences. Several chapters address the mechanobiology of collective and individual cell migration, including experimental, theoretical, and computational perspectives. Other chapters consider the crosstalk of biological, chemical, and physical cues in the tumor microenvironment, including the role of senescence, polyploid giant cells, TGF-beta, metabolism, and immune cells. Further, chapters focus on circulating tumor cells and metastatic colonization, highlighting both bioengineered models as well as diagnostic technologies. Further, this book features the work of emerging and diverse investigators in this field, who have already made impressive cross-disciplinary scientific contributions. This book is designed for a general audience, particularly researchers conversant in cancer biology but less familiar with engineering (and vice-versa). Thus, we envision that this book will be suitable for faculty, postdoctoral fellows, and advanced graduate students across medicine, biological sciences, and engineering. We also anticipate this book will be of interest to medical professionals and trainees, as well as researchers in the pharmaceutical and biomedical device industry.Describes physical aspects of cancer, including collective cell migration, the aberrant tumor microenvironment, circulating tumor cells, and metastatic colonization.First volume available on the topic of physical aspects of cancer

This book presents the modeling and scaling of physical problems, which result in normalized perturbation equations. This is followed by solving perturbation problems and evaluating the results. The author refines perturbation methods into simple, understandable elements and avoids unnecessary theorems and proofs. In addition, the results are consolidated and interpreted, and the presented examples are succinct to illustrate the essential techniques. This book is ideal and beneficial for practicing scientists and engineers who need to understand and apply perturbation methods to difficult problems with applications in mathematics, engineering, and biology. Discussions on new perspectives, simpler presentations on convergence, and the expansion of integrals are included.

This book gathers the latest advances, innovations, and applications in the field of sensing technology, as presented by international researchers and engineers at the 15th International Conference on Sensing Technology (ICST), held in Sydney, Australia on December 5¿7, 2022. Contributions include a wide range of topics such as: vision sensing, sensor signal processing, sensors phenomena and modelling, sensor characterization, smart sensors and sensor fusion, electromagnetic, chemical and physical sensors, electronic nose technology, biosensors, nano sensors, wireless sensors and WSN, Internet of Things, optical sensors, sensor arrays, intelligent sensing, Internet-based and remote data acquisition. The contributions, which were selected by means of a rigorous international peer-review process, present a wealth of exciting ideas that will open novel research directions and foster multidisciplinary collaboration among different specialists.

This book gathers papers presented at the 8th International Conference on Movement, Health and Exercise (MoHE 2022) with the conference theme "Enhancing Health and Sports Performance by Design". The topics covered include exercise science; human performance; physical activity and health; sports medicine; sports nutrition; management and sports studies; and sports engineering and technology. Its content is of interest to sports scientists, researchers and practitioners from various sports and exercise sub-disciplines.

This book highlights the emerging paradigm of nanomedicine, intersecting two burgeoning fields of nanotechnology and medicine. Numerous publications have appeared in the literature over the years, especially in cancer nanomedicine. In a boarder sense, nanomedicine aims to apply the knowledge and tools of nanotechnology in a mission to diagnose early and prevent or treat diseases using biocompatible nanoparticles (NPs). Current research in nanomedicine and its prospects depend on creating new breakthroughs at the nexus of nanomaterials and biological systems, making use of non-toxic NPs and nano/biomaterials as smart theranostic systems for a variety of diseases including cancer, neurodegenerative, orthopedic, and cardiac diseases. This book provides a review on recent advancements of nanomedicine in the aforementioned emerging areas of nanomedicine.¿

"In this unique and ambitious work that spans all of human history and looks boldly into its future, Reese melds science and history to look at the human species from a fresh new perspective"--

The Kirkwood-Buff Theory of Solutions: With Selected Applications to Solvation and Proteins presents the Kirkwood-Buff (KB) Theory of solution in a simple and didactic manner, making it understandable to those with minimal background in thermodynamics. Aside from the fact that the KB Theory may be the most important and useful theory of solutions, it is also the most general theory that can be applied to all possible solutions, including aqueous solutions of proteins and nucleic acids. Introductory chapters give readers grounding in the necessary chemical thermodynamics and statistical mechanics, but then move to a systematic derivation of Kirkwood-Buff theory and its inversion. Originally published in 1951, the KB theory was dormant for over 20 years. It became extremely useful after the publication of the "Inversion of the KB theory" by the author Arieh Ben-Naim in 1978. The book explains all necessary concepts in statistical mechanics featured in the theory in a simple and intuitive way. Researchers will find the theory useful in solving any problem in mixtures or solutions in any phase. Some examples of applications of the KB theory, to water, aqueous solutions, protein folding, and self-association of proteins, are provided in the book.

Chemical Theory and Multiscale Simulation in Biomolecules: From Principles to Case Studies helps readers understand what simulation is, what information modeling of biomolecules can provide, and how to compare this information with experiments. Beginning with an introduction to computational theory for modeling, the book goes on to describe how to control the conditions of modeling systems and possible strategies for time-cost savings in computation. Part Two further outlines key methods, with step-by-step guidance supporting readers in studying and practicing simulation processes. Part Three then shows how these theories are controlled and applied in practice, through examples and case studies on varied applications. This book is a practical guide for new learners, supporting them in learning and applying molecular modeling in practice, whilst also providing more experienced readers with the knowledge needed to gain a deep understanding of the theoretical background behind key methods.

Humans are electric beings. We are managed, monitored, and stimulated electrically. This textbook provides students and practitioners with a solid foundation and understanding of human electricity and the work currently being done to further develop electrical signals for medical purposes and related goals. The book introduces the fundamentals of how biological systems generate electrical signals, covering a wide range of biomedical engineering topics including bioelectricity, biomedical signals, neural engineering, and brain-computer interface. The book is presented in three sections: Part I explains how electrical signals and impulses manage the human body; Part II examines the kinds of electrical signals from the human body and how they are monitored, controlled, and used; Part III looks at clinical use of electrical stimulation toward the human body and how they are being developed for interventions in medicine. The book is also a valuable professional reference for practicing engineers and scientists. Explains humans as electric beings who are managed, monitored, and stimulated electrically;Deals with the electricity of major human organs;Covers a wide range of biomedical engineering topics

This book focuses on the dynamic complexity of neural, genetic networks, and reaction diffusion systems. The author shows that all robust attractors can be realized in dynamics of such systems. In particular, a positive solution of the Ruelle-Takens hypothesis for on chaos existence for large class of reaction-diffusion systems is given. The book considers viability problems for such systems - viability under extreme random perturbations - and discusses an interesting hypothesis of M. Gromov and A. Carbone on biological evolution. There appears a connection with the Kolmogorov complexity theory. As applications, transcription-factors-microRNA networks are considered, patterning in biology, a new approach to estimate the computational power of neural and genetic networks, social and economical networks, and a connection with the hard combinatorial problems.

X-ray computed tomography (CT) has been one of the most popular diagnostic imaging modalities for decades in the clinic for saving patients' life or improving their quality of life. This book is an introductory "one-stop shop" for technological and clinical topics in multi-detector Computed Tomography (MDCT).

This detailed volume is devoted to the recent development of quantitative experiments and computational methods driving new transforming growth factor beta (TGF-¿) and other cell signaling knowledge. Many chapters cover quantitative assays for TGF-¿ signaling studies, with others exploring the increasing role of both modeling and computational methods. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, TGF-Beta Signaling: Methods and Protocols serves as a vital resource for researchers seeking to move the TGF-¿ field into the quantitative realm.

Recently, the attention paid to self-care tests and the easy and large screening of a high number of people has dramatically increased. Indeed, easy and affordable tools for the safe management of biological fluids together with self-diagnosis have emerged as compulsory requirements in this time of the COVID-19 pandemic, to lighten the pressure on public healthcare institutions and thus limiting the diffusion of infections. Obviously, other kinds of pathologies (cancer or other degenerative diseases) also continue to require attention, with progressively earlier and more widespread diagnoses. The contribution to the development of this research field comes from the areas of innovative plastic and 3D microfluidics, smart chemistry and the integration of miniaturized sensors, going in the direction of improving the performances of in vitro diagnostic (IVD) devices.In our Special Issue, we include papers describing easy strategies to identify diseases at the point-of-care and near-the-bed levels, but also dealing with innovative biomarkers, sample treatments, and chemistry processes which, in perspective, represent promising tools to be applied in the field.