Genetik (ikke-medicinsk)

This volume discusses protocols that cover synthesis, screening by selection, and analysis of DNA-encoded chemical libraries (DEL). Chapters in this book focus on methods used to practice DEL technology and include solution phase library synthesis using a variety of chemistries; DNA encoding of chemical structure; design, preparation and analysis of target proteins and tool compounds; screening of soluble protein targets by affinity selection; DEL qPCR, preparative PCR and DNA sequence analysis; computational methods used to analyze selections and choose compounds for resynthesis; and analysis of hit compounds. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and comprehensive, DNA-Encoded Chemical Libraries: Methods and Protocols is a valuable resource for scientists interested in DEL technology for drug discovery, and will contribute to the continued advancement in this important field.

This new edition offers a state-of-the-art and integrative vision of pharmacogenomics by exploring new concepts and practical methodologies focusing on disease treatments, from cancers to cardiovascular and neurodegenerative disorders and more. The collection of these theoretical and experimental approaches facilitates problem-solving by tackling the complexity of personalized drug discovery and development. Written by leading experts in their fields for the highly successful Methods in Molecular Biology series, the book aims to provide across-the-board resources to support the translation of pharmacogenomics into better individualized health care. Authoritative and up-to-date, Pharmacogenomics in Drug Discovery and Development, Third Edition aims to aid researchers in approaching the challenges in pharmacogenomics and personalized medicine with the introduction of these novel ideas and cutting-edge methodologies.

This volume provides essential and fundamental protocols on manipulation chromosome. Chapters details methods on the preparation of mitotic chromosome, chromosome aberration, micronucleus (MN), comet assay, karyotyping, Fluorescent in situ hybridization (FISH), premature chromosome condensation (PCC), immunohistochemistry (IHC) staining, new generation sequencing technology and new chromosome concepts, such as epigenetic and its cause of cancer are presented. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Chromosome Analysis: Methods and Protocols aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge. 

Primate Anatomy: Introduction to Extant Primates synthesizes the taxonomy, anatomy, physiology, and genomics of extant primates, including humans. It takes a holistic approach to describing primate skeletal, muscular, and organ structure and function, providing the tools to understanding the fundamentals and state of the science of primatology. Now in its fourth edition, this work introduces its audience to the history and objectives of the field of primatology. It enumerates and profiles extant primates before delving into detailed descriptions of primate skulls, brains, teeth, skeletons, musculature, organs, blood groups, reproduction, and development. In addition, the book highlights recent advances in primate genomics, including new genera and species assignments, and concludes with a glance at the future of the field. Written by longtime expert Friderun Ankel-Simons, this new edition effectively introduces complex biological concepts in a manner accessible to all readers. It will serve as an excellent reference for technical and non-technical audiences, including primatologists, anatomists, paleontologists, anthropologists, conservationists, and naturalists.

Health Neuroscience is a new interdisciplinary field encompassing research from cognitive, affective, and social neuroscience, health psychology, physical and mental health, and science of behavior change. This new field addresses the longstanding gap among neuroscience, health, and behavior change within the context of health promotion.Fundamentals of Health Neuroscience explores key topics and research, including basic principles, psychological and neural processes, brain and body interactions, and gene x brain x environment interactions. This book also covers brain-based precision prevention and intervention strategies for health decisions and promotion across the lifespan. Chapters integrate the latest research findings and explores several key topics, such as: How does the brain serve both as a predictor and an outcome of health? How can people improve self-control and achieve physical and mental health effectively? What does brain plasticity and resilience tell us about optimal learning, performance, and development throughout our life? How is the sense of meaning in life affected by dopamine and reward systems in the brain?

This book presents deliberations on molecular and genomic mechanisms underlying the interactions of crop plants to the abiotic stresses caused by heat, cold, drought, flooding, submergence, salinity, acidity, etc., important to develop resistant crop varieties. Knowledge on the advanced genetic and genomic crop improvement strategies including molecular breeding, transgenics, genomic-assisted breeding, and the recently emerging genome editing for developing resistant varieties in vegetable crops is imperative for addressing FHNEE (food, health, nutrition, energy, and environment) security. Whole genome sequencing of these crops followed by genotyping-by-sequencing has provided precise information regarding the genes conferring resistance useful for gene discovery, allele mining, and shuttle breeding which in turn opened up the scope for 'designing' crop genomes with resistance to abiotic stresses.The nine chapters each dedicated to a vegetable crop or crop group in this volume elucidate on different types of abiotic stresses and their effects on and interaction with the crop; enumerate on the available genetic diversity with regard to abiotic stress resistance among available cultivars; illuminate on the potential gene pools for utilization in interspecific gene transfer; present brief on classical genetics of stress resistance and traditional breeding for transferring them to their cultivated counterparts; depict the success stories of genetic engineering for developing abiotic stress-resistant crop varieties; discuss on molecular mapping of genes and QTLs underlying stress resistance and their marker-assisted introgression into elite varieties; enunciate on different genomics-aided techniques including genomic selection, allele mining, gene discovery, and gene pyramiding for developing adaptive crop varieties with higher quantity and quality of yields, and also elaborate some case studies on genome editing focusing on specific genes for generating abiotic stress-resistant crops

This book explores recent advances in the microbial production of xylitol and its applications in food and medical sector. Xylitol is an important biomolecule from lignocellulose biorefinery which is produced from the xylose by chemical reactions or microbial fermentation methods. Currently, the demand of xylitol at commercial scale is being met through chemical methods. However, recent breakthroughs made in plant cell wall destruction, genetic engineering to develop the designer microorganisms, fermentation methods and media formulations and downstream processing have led the ways for sustainable production of xylitol at commercial scale in lignocellulose biorefineries. Microbial production of xylitol is preferred over the chemical processes as it is environmentally friendly, higher process efficiency with the desired product yield, and product recovery with minimum impurities. This book is a unique compilation of 11 book chapters written by experts in their respective fields. These chapters present critical insights and discuss the current progress and future progress in this area into fermentative xylitol production.Chapter 9 is licensed under the terms of the Creative Commons Attribution 4.0 International License. For further details see license information in the chapter.

This second edition volume expands on the previous edition with updated techniques and discussions on topics such as gene suppression, editing, and reprogramming; cardiac gene therapy vectors and promoters; cardiac gene delivery methods; pulmonary hypertension; and patient screening and measuring the efficacy of cardiac gene therapy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Cardiac Gene Therapy: Methods and Protocols, Second Edition is a valuable tool for researchers in cardiology who are conducting gene therapy research. This book will lead to further advancements and successful clinical translations in the field. Chapter "e;Direct Reprogramming of Adult Human Cardiac Fibroblasts into Induced Cardiomyocytes Using miRcombo"e; is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.