Genetik (ikke-medicinsk)

Various parallels have been drawn between wolves and humans from the perspective of their social organisation. Therefore, studying wolves may well shed light on the evolutionary origins of complex human cognition and, in particular, on the role that cooperation played in its development. Humans closely share their lives with millions of dogs - the domesticated form of wolves. Biologically, wolves and dogs can be considered to be the same species; yet only dogs are suitable living companions in human homes, highlighting the importance of cognitive and emotional differences between the two forms. The behaviour of wolves and dogs largely depends on the environment the animals grew up and live in. This book reviews more than 50 years of research on the differences and similarities of wolves and dogs. Beyond the socio-ecology, the work explores different theories about when and how the domestication of wolves might have started and which behaviours and cognitive abilities might have changed during this process. Readers will discover how these fascinating animals live with their conspecifics in their social groups, how they approach and solve problems in their daily lives and how they see and interact with their human partners.

This edited book is an in-depth compilation of recent tools and techniques, concepts and strategies used globally in plant molecular farming (PMF) for the cost-effective bulk production of recombinant proteins, secondary metabolites, and other biomolecules. The book presents an overview of success stories of PMF applications from developing countries to address poverty, achieve zero hunger, good health and well-being, thus achieving the UN SDGs 1, 2, and 3.The book deep dives into recent extraction and downstream processing methodologies, its co-existence with conventional agriculture, global governance and finally opportunities, challenges, and future perspectives in plant molecular farming. It focuses on plastid/chloroplast transformation (transplastomics) and its application in plant molecular farming. The books highlight recent advances in genome editing, synthetic biology, glycosylation and glyco-engineering for improved plant molecular farming by marker-free andtissue-specific systems via cisgenic and transgenic crops. In depth discussions on biosafety issues and bio-containment strategies have also been included.The book has 15 chapters authored by globally leading experts on the subject, presenting opportunities & challenges for bio-industrial researchers and entrepreneurs. It is useful to researchers, industrialists, entrepreneurs, policy planners, academician, and students across the disciplines.

The edited book brings out a comprehensive synthesis of latest scientific literature covering various important aspects of anaerobic biodigesters for human waste management that ranges from latest understanding on fundamental concepts/mechanisms of anaerobic biodigestion, modern tools and techniques used in process evaluation, current strategies being recruited for the performance enhancement, and case studies/ success stories across the world on applications of biodigesters used in human waste treatment.The anaerobic biodigestion is a process of break-down of organic waste by anaerobic microorganisms in absence of the oxygen. This process has been conventionally used for treating various types of organic waste including sewage sludge. After optimizing various process parameters, researchers have developed anaerobic biodigesters that have been successfully used for human waste (nigh soil) treatment. The topic of human waste treatment assumes global significance in the wake of UN sustainable Development Goals (SDG) wherein SDG-6 specifically highlights the Sanitation for all by 2030. The anaerobic Biodigester technology has the potential to manage the human waste as well and can contribute immensely in achieving targets of UN-SDG-6. This book is of interest to researchers, academicians, scientists, policy officials and capacity builders. Also the book serves as additional reading material for undergraduate and graduate students of environmental Biotechnology. National and international biotechnologists, environmental engineers and sanitation experts also find this to be a useful read.

This contributed volume aims at bringing together all the genetic engineering tools for managing various types of crop pests. The main focus of this book is to explore the application of these tools in pest management. Major pest groups covered in this book are insects, mites and nematodes.The first section covers all major genetic tools and molecular approaches. The second section deals with genetic tools for of beneficial containing three chapters involving honey bees, silkworms and natural enemies. Next section deals with genetic interactions against pests in diverse geographical regions with special focus on Africa, Vietnam and Sri Lanka. Sections four and five addresses diverse aspects as management of pests, genetic behavior, gene expression, plasticity, pathways and interactions and options for mitigation of pests.It serves as a useful resource for professionals in the fields of entomology, agronomy, horticulture, ecology, and environmental sciences, as well as to agricultural producers and plant biotechnologists. 

This second edition volume expands on the previous edition with discussions about the latest viral metagenomics aspects covering a range of different specimens such as soil, freshwater, wastewater, fecal samples, blood plasma, clinical tissues, fungi, and herbarium samples. Chapters also look at different viral groups including archaeal viruses, eukaryotic viruses, phages, mycoviruses, and circular DNA viruses. Techniques required for studying the three viral metagenomic steps of samples processing, library construction, and analysis of data are also discussed. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Vital Metagenomics: Methods and Protocols, Second Edition is a valuable resource for researchers who are interested in learning more about this important and developing field.

This updated volume explores Candida and candidiasis methods, useful to a wide variety of Candida scientists including those new to the field. Beginning with a section on the Candida genome, the book continues by covering techniques for antifungal discovery and studying resistance, pathogenesis and virulence, communities, as well as immunity. Biofilm protocols are also featured. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Candida Species: Methods and Protocols, Second Edition serves as an ideal guide for researchers working to further our understanding of this family of infective fungi.

The major purpose of this book is to present Johann Gregor Mendel (1822-1884) in a real and interesting way based on the most recent historical research and analysis of authentic sources. The authors aim to show Mendel's scientific thinking and inner feelings together with his environment and to communicate his message as a multifaceted personality and modern experimentalist.The book draws from the only existing short sketch of Mendel's youth, his letters and the biographical ceiling paintings that were made according to his proposal. They form the basis of the self-portrait concept. The structure of the book follows thematic groups covering Mendel's activities from a poor village boy in search for education and financial security, as not being physically suitable for running his father's farm. The book does not perpetuate the myths invented by some creative authors to make Mendel's biography more attractive. Mendel's life and work are dramatic enough without those embellishments. Mendel found happiness in science and he was able to explain the theory of new scientific facts. He was not a tragic figure, he did not work to become famous, but to be useful. His pea research has now been appreciated as a genius accomplishment of a scientist. The book is published at the occasion of Mendel's birthday bicentennial.

This third edition expands on the previous editions by providing updated protocols and current methods that cover recent breakthroughs in Drosophila research. Chapters guide readers through FlyBase, versatile gene expression systems, analysis of microRNA function, single-cell transcriptome data and metabolism, recent applications of CRISPR for precise genome editing, transcriptional activation and cell lineage tracing, protein inhibition and tagging, optogenetic and optochemical control of tissue mechanics, AFM measurements, sample preparation for electron microscopy, live imaging of different tissues and organs, and quantitative image analysis. Written in the format of the highly successful Methods in Molecular Biology series, chapters include an introduction to their topic, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls Authoritative and cutting-edge, Drosophila: Methods and Protocols, Third Edition serves as a useful and practical guide to new researchers and experts using Drosophila as a model system.Chapter 7 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Pharmacoepigenetics, Second Edition, Volume Ten in the Translational Epigenetics series, is a comprehensive reference on the role of epigenetics and epigenomics in drug discovery and development. Here, leading international researchers from across academia, clinical settings, and the pharmaceutical industry discuss the influence of epigenetics and epigenomics in human pathology, epigenetic biomarkers for disease prediction, diagnosis, and treatment, current epigenetic drugs, and the application of epigenetic procedures in drug development. Throughout the book, chapter authors offer a balanced and objective discussion of the future of pharmacoepigenetics and its crucial contribution to the growth of precision and personalized medicine.This new edition has been fully revised to address recent advances in epigenetics, from new natural and synthetic compounds with epigenetic effects to the role of epigenetics in the pathogenesis of a growing number of complex diseases, including further cancers, cardiovascular disorders, and brain disorders. Newly identified molecular components in the functional architecture of the epigenetic machinery, as well as practical and relevant pharmacoepigenetics topics related to COVID-19 and other world health challenges are also discussed.

Population Genomics in the Developing World: Concepts, Applications, and Challenges, a new volume in the Translational and Applied Genomics series, provides a comprehensive, up-to-date summary of the field of population genomics in developing countries. The book equips students, researchers, and clinicians with the practical skills and approaches necessary to face the unique challenges and opportunities of practicing population genomics in developing countries. Following a brief foundational overview, more than a dozen authors working in developing nations share applied case studies from the field, including rationale, methods, analysis, and outcomes to reinforce understanding. Key themes across the country specific chapters include efficient genetic data generation and effective computational and statistical tools to analyze population-level data. The book then discusses clinical interpretation of this data, from direct-to-consumer ancestry testing to translational and applied precision medicine. A final chapter considers ethical aspects of conducting genomic research in developing countries.

Plant Function Traits: Linking Climate and Ecosystem Functioning, part of the Plant Biology, Sustainability and Climate Change series, presents a wholistic understanding of Plant Functional Traits. As global climate change advances, natural resources are facing increasing survival challenges, hence this book directly addresses that need, exploring the morphological, physiological, and phenological properties of a plant that can be used as a proxy to understand plant environment interactions. Users will find great illustrations throughout individual chapters, along with case studies that demonstrate applications of functional traits in classifying vegetation of a region into distinct type groups as Plant Functional Types (PFTs). Additional information includes applications in the development of new generation of Dynamic Global Vegetation Model (DGVM) and an understanding of the response of vegetation to changing environments.

This book covers the wider aspects of the microbiology of hot desert soil ecosystems, compiling disparate information from a range of relevant desert soil microbial fields.The reader learns about microbial ecology of the more dominant and possibly most important desert habitats, detailing the phylogenetic and functional diversity of these different habitats as well as their potential role in desert ecosystem ecology. Particular attention is also given to microbial stress adaptation in hot desert soils. Furthermore, it is the first volume in this particular field to cover modern metagenomics technologies that can be applied to studies of all aspects of desert microbial communities. Additionally, the book explores viruses and viral communities, which are among the least studied (and little understood) components of desert soil microbial communities. Particular attention is also given to the roles of desert microbial communities in biogeochemical cycling of carbon, nitrogen and phosphorus. Through this book the reader discovers how desert microbiology has been at the forefront of Astrobiology and how it may be used conceptually in future terraforming strategies. Desert ecosystems are increasingly coming into focus given the impacts of climate change and desertification trends, making this volume particularly timely. Each of the chapters is authored by leading international researchers and is a must-read for microbial ecologists. 

Tauchen Sie ein in die Welt der Epigenetik, die Ihnen erklärt, wie Sie Ihre Gesundheit fördern und das Beste aus sich selbst herausholen können. Zunächst führt Sie das Werk in die molekularen Grundlagen ein, wie jede einzelne Zelle funktioniert und Information über ihre Umgebung verarbeitet. Letzteres hat sehr viel mit Epigenetik zu tun, die ein zentrales Thema in diesem Buch ist. Die Epigenetik, die für die Identität der mehr als 400 verschiedenen Zelltypen unseres Körpers entscheidend ist, hat auch einen dynamischen Aspekt. Sie lernen, wie intrazellulärer Signalübertragungswege über extrazelluläre Signale aktiviert werden und damit Transkriptionsfaktoren und Chromatin-modifizierenden Enzymen beeinflussen. Viele dieser Signale stammen direkt oder indirekt von unserer Ernährung. Letztere ist das wichtigste externe Signal, dem wir täglich ausgesetzt sind. Das führt zum zweiten zentralen Thema dieses Buches, der Nutrigenomik. Die Nahrung, die wir täglich zu uns nehmen, ist weit mehr als eine Ansammlung von Kohlenhydraten, Proteinen, Lipiden, Vitaminen und Mineralien, die Energie und Bausteine für unseren Körper liefert. Das faszinierende Fachgebiet der Nutrigenomik beschreibt die Wechselwirkung zwischen Nahrungsmolekülen, ihren Metaboliten und unserem (Epi)genom. Viele Nahrungsbestandteile, wie beispielsweise ungesättigte Fettsäuren, aktivieren direkt Transkriptionsfaktoren oder verursachen Veränderungen in den Konzentrationen von Metaboliten. Diese beeinflussen wiederum die Aktivität von Chromatin-modifizierenden Enzymen. Auf diese Weise ¿sprechen¿ Moleküle unseres Frühstücks, Mittagessen oder Abendessen mit unserem Genom bzw. Epigenom. Tauchen Sie ein in die faszinierende Welt der Selbstheilung und Verantwortung für Ihre Gesundheit!

This volume provides new approaches and technologies into roles of poly(A) metabolism in translation, RNA stability, and quality control of gene expression. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Deadenylation: Methods and Protocols aims to pave the way for future investigations of the complex regulatory networks that control mRNA stability and expression.

This volume presents a collection of versatile methodologies to investigate prokaryotic gene regulation, with focus on the different levels of information processing and usefulness for various model organisms, whether archaeal, bacterial, or both. The chapters in this book are divided into four sections. Section One covers methods that enable the study of the structure of the bacterial/archaeal chromosome, the main template for all gene regulatory processes, and its epigenetic modification. Section Two looks at a selection of approaches that enable higher levels of understanding of transcription initiation, a key step in information processing. Section Three discusses the investigation of regulating transcription factors, which are often considered the main players in gene regulation in prokaryotic cells. The Fourth Section focuses on the next stage of information processing at which gene regulation occurs, namely the RNA-based level. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and comprehensive, Prokaryotic Gene Regulation: Methods and Protocols is a valuable resource for researchers interested in learning more about this diverse field.

Global food security is increasingly challenging in light of population increase, the impact of climate change on crop production, and limited land available for agricultural expansion. Plant breeding and other agricultural technologies have contributed considerably for food and nutritional security over the last few decades. Genetic engineering approaches are powerful tools that we have at our disposal to overcome substantial obstacles in the way of efficiency and productivity of current agricultural practices. Genome engineering via CRISPR/Cas9, Cpf1, base editing and prime editing, and OMICs through genomics, transcriptomics, proteomics, phenomics, an metabolomics have  helped to discover underlying mechanisms controlling traits of economic importance.     Principle and Practices of OMICs and Genome Editing for Crop Improvement provides recent research from eminent scholars from around the world, from various geographical regions, with established expertise on genome editing and OMICs technologies. This book offers a wide range of information on OMICs techniques and their applications to develop biotic, abiotic and climate resilient crops, metabolomics and next generation sequencing for sustainable crop production, integration bioinformatics, and multi-omics for precision plant breeding. Other topics include application of genome editing technologies for food and nutritional security, speed breeding, hybrid seed production, resource use efficiency, epigenetic modifications, transgene free breeding, database and bioinformatics for genome editing, and regulations adopted by various countries around globe for genome edited crops. Both OMICs and genome editing are vigorously utilized by researchers for crop improvement programs; however, there is limited literature available in a single source. This book provides a valuable resource not only for students at undergraduate and postgraduate level but also for researchers, stakeholders, policy makers, and practitioners interested in the potential of genome editing and OMICs for crop improvement programs.

This book is the first attempt for in-depth compilation of current knowledge on cisgenic crops and their  potential prospects as a sustainable substitute for the controversial genetically modified crops. Innovative methodologies for the development of cisgenic crops for disease resistance, improved nutritional contents, suitability for organic farming, survival under climate change, and their role in conservation of plant genetic resources have been highlighted. Combined with molecular markers and genome editing, an advanced approach for crop improvement is reported. The book has 14 chapters authored by globally leading experts on the subject. This book is useful to the students, teachers, researchers and policy planners working across the disciplines of classical plant breeding up to the recent genetically modified and genome edited crops.

This detailed volume collects commonly used and cutting-edge methods to analyze alternative splicing, a key step in gene regulation. After an introduction of the alternative splicing mechanism and its targeting for therapeutic strategies, the book continues with techniques for analyzing alternative splicing profiles in complex biological systems, visualizing and localizing alternative spliced transcripts with cellular and sub-cellular resolution, probing regulators of alternative splicing, as well as assessing the functional consequences of alternative splicing. Written for the highly successful Methods in Molecular Biology series, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Alternative Splicing: Methods and Protocols serves as an ideal guide for both RNA aficionados that want to implement novel approaches in their labs and novices undertaking alternative splicing projects.

This book focuses specifically on environmental risk assessment in premetamorphic stages and adults of amphibians and is an ideal companion for toxicologists and ecologists also of interest to those working in conservation biology, biological invasion, biocontrol and habitat management.

This volume details the development of updated dry lab and wet lab based methods for the reconstruction of Gene regulatory networks (GRN). Chapters guide readers through culprit genes, in-silico drug discovery techniques, genome-wide ChIP-X data, high-Throughput Transcriptomic Data Exome Sequencing, Next-Generation Sequencing, Fuorescence Spectroscopy, data analysis in Bioinformatics, Computational Biology, and S-system based modeling of GRN. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Reverse Engineering of Regulatory Networks aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.

This detailed volume explores a variety of cutting-edge techniques used to interrogate spatial genome organization. Beginning with a section covering the vital chromosome conformation capture (3C) technique, this collection continues with chapters on targeted Hi-C approaches, sequencing-based approaches to assess nuclear environment, as well as single-cell technologies to better characterize the heterogeneity and dynamics of nuclear architectures and approaches to visualize them by microscopy. Finally, in order to be able to ask functional questions about the role of spatial chromatin organization in genomic control, the last section provides methods for acute manipulations of chromatin architecture. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Spatial Genome Organization: Methods and Protocols is an ideal resource for researchers searching for the best techniques to address their own specific research questions.

Biotic stresses cause yield loss of 31-42% in crops in addition to 6-20% during post-harvest stage. Understanding interaction of crop plants to the biotic stresses caused by insects, bacteria, fungi, viruses, and oomycetes, etc. is important to develop resistant crop varieties. Knowledge on the advanced genetic and genomic crop improvement strategies including molecular breeding, transgenics, genomics-assisted breeding and the recently emerging genome editing for developing resistant varieties in vegetable crops is imperative for addressing FPNEE (food, health, nutrition. energy and environment) security. Whole genome sequencing of these crops followed by genotyping-by-sequencing have facilitated precise information about the genes conferring resistance useful for gene discovery, allele mining and shuttle breeding which in turn opened up the scope for 'designing' crop genomes with resistance to biotic stresses.The nine chapters each dedicated to a vegetable crop or crop-group in this volume will deliberate on different types of biotic stress agents and their effects on and interaction with crop plants; will enumerate on the available genetic diversity with regard to biotic stress resistance among available cultivars; illuminate on the potential gene pools for utilization in interspecific gene transfer; will brief on the classical genetics of stress resistance and traditional breeding for transferring them to their cultivated counterparts; will enunciate the success stories of genetic engineering for developing biotic stress resistant varieties; will discuss on molecular mapping of genes and QTLs underlying biotic stress resistance and their marker-assisted introgression into elite varieties; will enunciate on different emerging genomics-aided techniques including genomic selection, allele mining, gene discovery and gene pyramiding for developing resistant crop varieties with higher quantity and better quality; and will also elaborate some case studies on genome editing focusing on specific genes for generating disease and insect resistant crops.

This edited book covers all aspects of omics approaches used for the varietal improvement of millets in changing climatic conditions. Millets are the collection of small-grained cereal grasses, that are grown for human carbohydrate needs. They are among the oldest crops, mainly divided into two groups - Major and small millets based on seed size. Small millets are earlier considered orphan crops, but recently due to their nutritional values, they are getting importance in cultivation. This book explores the genomics, transcriptomics, proteomics, metabolomics, bioinformatics, and other omics tools that are being widely used to get a clear understanding of mechanistic approaches taken by plant genes to tolerate stress. Various reports are published based on field breeding on these crops, and recently the genome of some of the small millets is released, and many omics studies are published related to its application in varietal improvements. This book reviewed all those recent studies and is of interest to research students, plant breeding scientists, teachers that are working in agriculture and plant biotech universities. Along with this, the book serves as reference material for undergraduate and graduate students of agriculture, and biotechnology. National and international agricultural scientists, policymakers will also find this to be a useful read.