Biokemi

"Beyond the Beats: Your Comprehensive Guide to Cardiovascular Health," a book delving into the complexities of the human heart and cardiovascular disorders. The project originated from the recognition of the global impact of cardiovascular diseases, emphasizing the need for a thorough understanding of their multifaceted causes. The book takes readers on a detailed journey through the anatomy, physiology, and pathology of the heart, offering a sophisticated grasp of its structure and operation. Organized to immerse readers progressively, it explores the circulatory system, cardiovascular health's role in overall well-being, and various disorders, incorporating innovative studies. While intended for academics and medical professionals, the book aims for accessibility to a wider audience, fostering cross-disciplinary discourse. The authors hope their thorough investigation contributes to cardiovascular science knowledge and the broader dialogue on disease prevention and public health.

With one volume each year, this series keeps scientists and advanced students informed of the latest developments and results in all areas of the plant sciences. This latest volume includes reviews on plant physiology, biochemistry, genetics and genomics, forests, and ecosystems.

This brief is a concise guide that explores the theory and practical aspects of improving enantioselectivity in enzymatic kinetic resolution, with a specific focus on the role of hydrolases in this process. Enzymatic kinetic resolution is a valuable technique for obtaining enantiopure compounds, and hydrolases are highlighted as a crucial class of enzymes widely used in industries for chiral synthesis involving kinetic resolution. The book emphasizes the importance of tuning and optimizing the enzymatic kinetic resolution process to achieve the highest possible enantiomeric excess in the final product or starting material while maintaining the desired yield. Through illustrative examples, the text aims to make the concept accessible and appealing to graduate students, researchers, and young organic chemists seeking to incorporate hydrolases in their stereoselective synthesis endeavors.

This book overviews ongoing and upcoming clinical applications of basic immunology. Recent advances in our knowledge of immunology coupled with new technologies have aided in the development of efficient cancer immunotherapy, as well as the control of emerging microorganisms such as SARS-CoV-2. However, knowledge of basic immunology has not been fully utilized even after the discoveries of immune checkpoint inhibition for cancer immunotherapy and the development of mRNA vaccination against SARS-CoV-2. There is still room for improving the clinical application of basic immunology. The book summarizes the achievements in clinical applications of basic immunology and highlights what can be further extended to make immunology a more practical human science. Basic immunology and its clinical applications are two wheels of the same cart in the immunology field, which aids in the development of more efficient cancer immunotherapy and rapid control of infectious diseases against microorganisms, including new viruses and classical toxoplasmosis. The exploration of ongoing and upcoming applications of basic immunology in this book makes it a useful resource for immunologists, physicians, molecular and genome biologists, bioinformaticians, and students in these fields.

This volume explores the latest techniques and protocols used by researchers to address unique biological questions, model organisms not typically studied by Fluorescent In Situ Hybridization (FISH), protocols combining FISH with immunofluorescence (FISH-IF), and high-throughput experiments. The chapters in this book are divided into two parts: RNA FISH protocols and DNA FISH protocols. Part One covers methods for designing OligoPaint probes and studying distinct aspects of RNA biology such as transcription and splicing dynamics, and mRNA and small RNA expression and localization. Part Two discusses DNA repair dynamics, gene compaction, and chromatin conformation and gene rearrangements in plants, insects, and mammalian cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting edge and thorough, Fluorescence In Situ Hybridization (FISH): Methods and Protocols is a valuable resource that will benefit the broader scientific community in their studies and understanding of this important field.

Enhancing Aspirin Repositioning with Cisplatin in Glioma is a comprehensive book written by Abirami M P, which explores the potential of using aspirin and cisplatin in combination as an effective treatment for glioma, a type of brain cancer.The book provides a detailed molecular study of the mechanisms by which aspirin and cisplatin work together to induce cell death in glioma cells. Abirami M P highlights the benefits of repositioning aspirin, a widely used anti-inflammatory drug, in combination with cisplatin, a potent anticancer drug, for the treatment of glioma.The book also discusses the challenges associated with the development of effective therapies for glioma and how the use of aspirin and cisplatin can help overcome these challenges. Abirami M P provides a thorough analysis of the current research in the field and offers insights into the future directions of research.The book is written in a clear and concise manner, making it accessible to a wide range of readers, including researchers, clinicians, and students in the field of oncology. It provides a valuable resource for those interested in understanding the potential of aspirin and cisplatin in the treatment of glioma and other types of cancer. Overall, Enhancing Aspirin Repositioning with Cisplatin in Glioma is an essential read for anyone interested in the latest advancements in cancer treatment research.

The term nutraceuticals is applied to the products isolated from the herbal sources, nutrients or dietary supplements, processed foods, (cereals, soup and beverages) and specific diets, that may also used as medicine other than nutrition. It usually refers to the foods derived products which may sell in medicinal form, possessing physiological benefits to the host while promoting health and wellness and provide protection against chronic disorders. They may also increase the life expectancy, delay the aging and/or support the structure and function of the body organs. Demand for nutraceuticals with potential to prevent and treat chronic disorders has been interestingly increasing over the past few years. Based on food sources, nutraceuticals are classified into dietary fibers, probiotics, prebiotics, polyunsaturated fatty acids (PUFAs), vitamins, polyphenols and spices. A dietary supplement is generally considered as product containing vitamins, minerals, amino acids, and medicinal plants that are indented to be used to supplement the diet or a concentrate, metabolite, constituent, extract, or combinations of these ingredients. Broadly, nutraceuticals are any of these ingredients or supplements using for health purposes other than nutrition. This book on the fundamentals of Nutraceuticals is organized in two parts i.e., the introductory aspects of macro- and micro-nutrients, gut microbiota regulation, nutraceuticals and drug interactions, while the second part is focusing on the biological benefits of nutraceuticals in different disease states. Besides, this book is also addressing the safety aspects of nutraceuticals and phytotherapeutic support in pregnancy. Including contributions from the experts in the field, target audience (pharmacists, nutritionists, other healthcare providers, and researchers in food and nutrition sector) will be benefited with the updated literature on nutraceuticals and dietary supplements.

Nanotechnology, a rapidly expanding field, showcases diverse applications across disciplines. Platinum nanoparticles (PtNPs) are a focal point in scientific exploration, offering non-toxicity and stability, especially in bioengineering. This concise review surveys global research on PtNPs, covering synthesis methods (Physical, Chemical, and Biological), along with in-vivo and in-vitro analyses. Nanometrological techniques for characterization are explored, aiding in understanding their physical and chemical attributes. The book extends to recent advancements in PtNP functionalization for biomedical purposes, including nanomedicine, combination therapy, cancer treatment, and antibacterial applications. The structured framework of the book comprises sections on Introduction, Platinum Nanoparticles, Synthesis, Characterization, Toxicology, Biomedical Industry, Antibacterial Applications, and a concluding perspective.

This detailed volume explores poly(ADP-ribose) polymerases (PARPs) in the biology of eukaryotes and their relevance to human health. Beginning with a section on the detection and quantification of poly(ADP-ribose) polymer (pADPr), the book continues by delving into the identification of protein targets, functional analysis, the poly(ADP-ribosyl)ating pathway in chromatin and genes expression, as well as the use of animal models and PARP1 inhibitor design and testing, and more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Poly(ADP-Ribose) Polymerase: Methods and Protocols, Third Edition presents essential new and classical methods for studying the pADPr-pathway.

This book describes the development of novel protein¿RNA-binding assays and their applications in a high-throughput manner for the identification of small-molecule modulators of protein¿RNA interactions to treat cancer and COVID-19.Modulating protein¿RNA interactions with small molecules is expected to provide novel biological insights of the interrelation of diseases with the protein¿RNA interactome. The modulations may also be exploited therapeutically. For these reasons, the development of a simple, reliable, and sensitive protein¿RNA-binding assay is necessary for high-throughput screening to discover new effective chemical entities capable of acting on diverse protein¿RNA interactions. This book discusses the discovery of small-molecule modulators targeting protein¿RNA interactions that are potentially valuable to treat cancer and COVID-19 by constructing novel high-throughput screening methods. The results of this dissertation provide valuable insights into the regulation of protein¿RNA interactions in chemical biology and drug development.

This book focuses on the application and possibility of field-effect transistors (FETS) as biosensors, for rapid and real time detection of pathogens that affect human life.

This detailed volume explores experimental strategies and protocols for the expression, assembly, characterization, and exploitation of transmembrane ¿-barrel proteins. Beginning with methodologies to study their assembly, the book continues with protocols for characterizing the landscape of transmembrane ¿-barrel protein interactions with other cellular factors, dissecting processes of protein transport in bacteria and mitochondria, examining structural characterization, determination, and prediction, and more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Transmembrane ¿-Barrel Proteins: Methods and Protocols serves as an ideal guide for researchers seeking to expand our knowledge of these vital membrane-spanning proteins.

This book is designed to serve as a textbook for core as well as elective courses offered to undergraduate and advanced undergraduate students enrolled in chemistry. This textbook comprehensively deals various topics of organic chemistry such as amino acids, peptides, proteins and enzymes. The text is divided into four chapters: a chapter each dedicated to amino acids, peptides, proteins and enzymes, respectively. The important reactions have been explained with the help of the mechanisms involved. It gives a detailed account of the solution phase and solid phase synthesis of peptides as well as discussing the structure and function of some biologically important peptides. It also covers the classification, nomenclature and mode of action of enzymes, and a detailed account of the structure and function of different co-enzymes. The book also includes pedagogical features like end-of-chapter exercises to aid in self learning. Given the scope, this textbook will be useful for graduate and advanced graduate students pursuing the course of chemistry, especially organic chemistry.

The book focuses on how sustainable nanomaterials can help with various aspects of infectious diseases, including prevention, diagnosis, and treatment, as well as the characteristics that materials should possess in order to enter clinical trials.

Biochemistry, a branch of chemistry that explores the chemical processes within living organisms, plays a crucial role in scientific research. In fact, it is difficult to overstate the importance of biochemistry in advancing our understanding of the natural world and improving human health. This subchapter delves into the significance of biochemistry in scientific research, aiming to inspire student scientists in the field of chemistry.Biochemistry serves as the foundation for numerous scientific disciplines. By studying the chemical reactions and processes that occur within living organisms, biochemists can unlock the secrets of life itself. This knowledge is then applied to diverse fields, such as medicine, agriculture, and environmental science, to name just a few.One of the primary reasons biochemistry is instrumental in scientific research is its ability to elucidate the mechanisms behind biological phenomena. From the intricate workings of DNA replication to the complex metabolic pathways that sustain life, biochemistry provides the key to understanding the building blocks of life. By comprehending these fundamental processes, scientists can develop targeted interventions to combat diseases, engineer novel enzymes for industrial applications, and even address global challenges like climate change.Furthermore, biochemistry plays a critical role in drug discovery and development. Through the study of biological macromolecules, such as proteins and nucleic acids, researchers can identify potential drug targets and design molecules that interact with them. This process, known as rational drug design, has revolutionized the pharmaceutical industry and led to the development of life-saving medications for various diseases.For students interested in pursuing a career in chemistry, biochemistry offers exciting opportunities for research and innovation. The field is constantly evolving, with new breakthroughs and discoveries occurring regularly. By studying biochemistry, students can contribute to cutting-edge research projects, collaborate with scientists from diverse backgrounds, and make meaningful contributions to scientific knowledge.

Today, its well established that genotoxic chemicals are widely distributed in drugs,pesticides, cosmetics, narcotics, air and water pollutants, food additives and foodcontaminants (Belpomme, 2007; Irigaray, 2007). Intake of DNA damagingenvironmental contaminants can lead to heritable genetic defects, cardiovascularproblems, diabetes, cancer, premature ageing, teratogenic effects andneurodegenerative diseases (Hoeijmakers, 2009; De Flora and Ramel, 1988; Fergusonand Philpott, 2008). Hence, there is a need to develop a suitable strategy to safeguardhuman genetic material from the catastrophic consequences of exposure toenvironmental toxicants.

Scaffold proteins assemble vanous signaling molecules into a signalosome and activate signal transduction cascade. These proteins thus provide platforms for receptors, kinases, phosphatases and other effectors into a functional modules (Good et al., 2011). Scaffold proteins are thus key components ofsignaling networks. Among various domain structures in scaffolding proteins, one commonly found is WD40 repeat. Proteins harboring this domain often act as signaling scaffold in which the WD40 repeat domain form a beta propeller structure for assembling signaling molecules.

This book deals with the recent advances in DNA-Encoded Library (DEL) technology that has emerged as an alternative to high throughput screening (HTS) over the last decade and has been heralded as a "e;disruptive"e; technology for drug discovery. The book aims to provide a comprehensive overview of all of the major components of the DEL process from conception to bench execution and clinical investigations. The contributions from experts in the field combine different perspectives from academia and industry. The book will be of interest to researchers in the drug discovery field as well as to graduate students and scholars who are interested in this rapidly improving technology.

This volume details protocols emphasizing systems-level approaches that can be applied to genomic analyses. Chapters detail techniques for optimized application in in vivo systems, spatial, physiological, environmental contexts, imaging-based techniques, single-molecule approaches, CRISPR systems, new genomic approaches, and measurements of kinetics governing. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, DNA-Protein Interactions: Methods and Protocols aims to present genome-wide techniques that will complement the biochemistry-based protocols to aid researchers in their studies. 

This updated edition explores essential techniques and newly emerged applications used in the expanding field of peptidomics. The opening chapters describe sample preparation and basic techniques. Subsequent chapters delve into quantitative peptidomics, bioinformatics, deep learning approaches, non-human model organisms, and peptide toxins. The last section includes multiple chapters on applications of peptidomics for human clinical specimens. Written for the highly successful Methods in Molecular Biology series, chapters include overviews and introductions to their respective topics, lists of the necessary materials, reagents, and instrumentation, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Peptidomics: Methods and Strategies, Second Edition serves as an ideal guide for state-of-the-art approaches in this vital field.

Abiotic stressors, such as drought, extreme temperatures, heavy metals, or high salinity, are causing huge crop losses worldwide. These abiotic stressors are expected to become more extreme, less predictable, and more widespread in the near future. The harm of abiotic stresses includes the disruption of cellular redox homeostasis, reactive oxygen species (ROS) production, and oxidative stress in the plant. Plants have different mechanisms to fight stress, and these mechanisms are responsible for maintaining the required homeostasis in plants. Research on gasotransmitters is rapidly expanding and knowledge regarding the potential of gasotransmitters in biology and medicine is accumulating. Over the past few decades, the roles of these signaling molecules, especially NO and H2S, have been extensively studied for their application in plants. Recently, the emissions of endogenous gasotransmitters in plants have been widely studied and analyzed, thereby providing information to facilitate our understanding of new gasotransmitters signaling pathways. Given the multidimensional role of these signaling molecules, research over the past decades in mitigating abiotic stresses in plant biology and from an agriculture point of view, we intend to bring forth a comprehensive volume ¿Gasotransmitters Signaling in Plants under Challenging Environment". We are hopeful that this comprehensive volume will furnish the requisite of all those who are working or have an interest in the proposed topic

This book delves into the world of natural sources such as medicinal plants, microbes, fungi, lichens, and algae that have been used for centuries in traditional medicine. The book provides a comprehensive guide to the isolation, characterization, and authentication of these natural sources for use in cosmeceuticals.