Molekylærbiologi

Molekylærbiologi og biokemi – Teori og metode behandler fagets grundlæggende teorier sammen med principperne for mange af de metoder, der anvendes i biokemiske og molekylærbiologiske laboratorier.Denne 3. udgave er en grundigt revideret udgave af "Biokemi og bioteknologi". Specielt er metodeafsnittene udvidede og ajourført. Til hvert kapitel er der desuden udarbejdet repetitionsspørgsmål. Bogen er primært skrevet med sigte på laborantuddannelsen og laboranternes efteruddannelse, men kan benyttes af alle, der skal introduceres til fagene biokemi og molekylærbiologi og som støtte i andre naturvidenskabelige uddannelser.

This book features chapters written by renowned scientists from various parts of the world, providing an up-to-date survey of submanifold theory, spanning diverse topics and applications. The book covers a wide range of topics such as Chen¿Ricci inequalities in differential geometry, optimal inequalities for Casorati curvatures in quaternion geometry, conformal ¿-Ricci¿Yamabe solitons, submersion on statistical metallic structure, solitons in f(R, T)-gravity, metric-affine geometry, generalized Wintgen inequalities, tangent bundles, and Lagrangian submanifolds. Moreover, the book showcases the latest findings on Pythagorean submanifolds and submanifolds of four-dimensional f-manifolds. The chapters in this book delve into numerous problems and conjectures on submanifolds, providing valuable insights for scientists, educators, and graduate students looking to stay updated with the latest developments in the field. With its comprehensive coverage and detailed explanations, this book is an essential resource for anyone interested in submanifold theory.

Delving into the recent discovery of the brain's immune system, Dr. Monty Lyman reveals the extraordinary implications for our physical and mental health.Until a decade ago, we misunderstood a fundamental aspect of human health. Although the brain and the body have always been viewed as separate entities--treated in separate hospitals--science now shows that they are intimately linked.Startlingly, we now know that our immune system is in constant communication with our brain and can directly alter our mental health.This has opened up a new frontier in medicine. Could inflammation cause depression, and could arthritis drugs cure it? Can gut microbes alter your mood? Can something as simple as brushing your teeth properly reduce your risk of dementia? Could childhood infections lie behind neurological and psychiatric disorders such as tics and obsessive compulsive disorder?In The Immune Mind, Dr. Monty Lyman explores the fascinating connection between the mind, immune system, and microbiome. A specialist in the cutting-edge field of immuno-psychiatry, Lyman argues that we need to change the way we treat disease and the way we see ourselves. For the first time, we have a new approach to medicine that treats the whole human being.

Anandamide in Health and Disease, a volume in the Molecular Mediators in Health and Disease: How Cells Communicate series, is a complete reference on the roles of anandamide in health and disease states. Following the characteristics of the series, this book provides a comprehensive overview of the effects of the mediator on human health.The book is divided into two parts, the first of which provides background information on biology, regulation, synthesis and degradation and modulation of anandamide. The second part is dedicated to covering the role of anandamide in various diseases. A comprehensive panel of authors cover several somatic and psychiatric disorders, such as anxiety disorders, mood disorders, psychosis and substance use disorders. Additionally, the role of anandamide across epilepsy, Alzheimer's disease, and digestive conditions is explored.Anandamide in Health and Disease is the perfect reference for bioscience researchers seeking to understand anandamide pharmacology, biochemistry, cellular, and molecular biology. Clinicians may also be interested in better understanding the wide-ranging health effects of the mediator.

This book explains the advancements of plant biotechnology and advanced molecular biology and explores the details of influential tools that complement conventional breeding and accelerate the development of plants resilient to adverse agroclimatic conditions and biofortified plants. Plant biotechnology from the basic sciences to current applications, such as pathway engineering, precursor feeding, transformation, elicitation with biotic and abiotic elicitors, and scaling up in bioreactors, have been included in these chapters to improve the production of secondary metabolites from different medicinal plants. It also highlights important factors often overlooked by methodologies used to develop plants' tolerance against biotic and abiotic stresses and in developing special foods, bio-chemicals, and pharmaceuticals. This book is valuable for researchers or students working on biosciences. It is also an updated and advanced reference material for the agriculture and pharmaceutical industries.

Nongenetic Information and Evolution investigates the origin and nature of nongenetic information and its role in the mechanisms of evolutionary change. This book opens with an introduction to the theoretical background and forms of nongenetic information, alongside its relationship with genetic information. It then explores nongenetic information across the biological kingdoms, including animals, nonneural organisms, plants, and unicellulars. It also covers epigenetics as a form of nongenetic information, exploring DNA methylation, histone modifications, chromatin remodeling, and miRNA expression in this context. This book closes with a discussion of nongenetic information in evolution, considering evidence demonstrating its inheritance and hereditary impact. This book provides a detailed overview of the origin and evolutionary impact of nongenetic inheritance, delivering a basis for further understanding the occurrence of hereditary phenomena and transgenerational phenotypic changes that do not involve genes. This is a useful reference for researchers in the field of epigenetics, evolutionary biology, developmental biology, and cellular and molecular biology.

The Charnolosome as a Novel Nanothereranostic Biomarker: Overcoming Future Challenges in Medicine provides an overview of the charnolosome and its potential as a biomarker of cell injury. Based on the author’s original discovery of the charnoly body in the developing, undernourished rat cerebellar Purkinje neurons, this book delves into the potential for utilizing this mitochondria and lysosomal-derived intracellular organelle as a nanotheranostic biomarker to prevent and cure various diseases. The book discusses the cellular, molecular, genetic, and epigenetic mechanisms of charnolosomes and charnolosome-derived nano-vesicles. It also investigates the molecular mechanisms underlying auto-inflammatory, autoimmune, and infectious diseases resulting from their compromised mitochondrial bioenergetics, and the potential use of the charnolosome in preventing and curing such conditions.

The Physics of Protein Structure and Dynamics looks at various aspects of protein structure and dynamics from a physico-chemical point of view. It goes into some depth regarding the description of non-covalent forces that determine the relative stability of folded and unfolded proteins. Anharmonic protein dynamics involving motions between different minima of a rugged Gibbs energy landscape is described in great detail. The book combines various aspects of the protein folding/unfolding processes with an overview of intrinsically disordered proteins, which have attracted considerable interest of the protein community over the last 25 years but are thus far underrepresented in classroom-oriented textbooks. The book looks at protein folding and intrinsically disordered proteins as heavily interrelated topics that need to be viewed together. Furthermore, it presents some basic physico-chemical aspects of protein/peptide self-assembly into nanoscale fibrils. Intrinsically disordered peptides and proteins play a major role particularly in aggregation and self-assembly processes that lead to various diseases (Alzheimer, Parkinson, Huntington, Mad-Cow). Therefore, the relevance of protein disorder for protein self-assembly deserves a closer look. Protein self-assembly cannot be separated from protein folding since it is frequently the product of misfolding. With regard to modern theories, the folding processes are linked to insights on protein dynamics and the discovered relationship between proteins and spin glasses.

Paper-based Optical Chemosensors comprehensively discusses the origin, development, and current state-of-the-art in paper-based sensors. With a focus on the principles, classifications, methodology, design, and application of paper-based sensors, this book represents a developing research field with recent innovative applications resulting in a comprehensive presentation of the different physico-chemical techniques using paper sensors. It discloses underlying rules and factors in paper-based sensors and discusses intricate sensing systems and working environments by ways of chemistry and physics for a variety of application scenarios such as environmental protection, food safety, public safety, and clinical diagnosis. This is a valuable resource for researchers who major in analytical chemistry, or for those who are interested in the development of methods or devices for rapid analysis/monitoring based on paper/membrane-based sensors who wish to broaden their knowledge in the allied field.

This brief offers an introduction to the fascinating new field of quantitative read-across structure-activity relationships (q-RASAR) as a cheminformatics modeling approach in the background of quantitative structure-activity relationships (QSAR) and read-across (RA) as data gap-filling methods. It discusses the genesis and model development of q-RASAR models demonstrating practical examples. It also showcases successful case studies on the application of q-RASAR modeling in medicinal chemistry, predictive toxicology, and materials sciences. The book also includes the tools used for q-RASAR model development for new users. It is a valuable resource for researchers and students interested in grasping the development algorithm of q-RASAR models and their application within specific research domains.

This 2 volume book is an up-to-date overview of current progress in Arbuscular Mycorrhizal Fungal (AMF) technique development, inoculum production and its quality regulations, application in agriculture, horticulture, agroforestry and other ecosystems along with nutrient management for sustainable food production. It offers new insight and cutting-edge information for novices and experts such as students, academicians, researchers, environmentalists, industrialists, and other individuals interested in the field of mycorrhiza.AMF provides favorable rhizospheric environment to the plant with numerous direct and indirect mechanisms, in exchange of soil nutrients and photosynthetically fixed carbon. Other than the species composition and diversity determination of natural ecosystem, AMF play a vital role in maintaining the soil quality, agricultural sustainability and environmental integrity. The second volume provide comprehensive knowledge on AMF role in nutrient cycling, nutrient exchange and their acquisition under normal and stress condition.

This edited book is a comprehensive compilation of applications of plant molecular farming in various fields including agriculture, industry and medicine, using different plant/crop systems as bioreactors for the bulk production of products of interest. The book places special emphasis on plant molecular farming being utilized for the bulk production of biologics, industrial enzymes, vaccines, drugs and recombinant pharmaceutical proteins.Plant molecular farming (PMF) is an upcoming promising interdisciplinary subject, and therefore the book contains contributions from experts across the disciplines. The utilization of plant viral vectors to produce biologics, virus-like particles and antimicrobial peptides via PMF have been highlighted. Molecular farming for the bulk production of recombinant pharmaceutical proteins and various types of human and veterinary vaccines in different crops/plants and genetically modified seeds has been included. The process of scaling up to manufacturing capacity, product formation by bioprocessing of plant cell suspension culture, and bioreactor engineering have been incorporated. Lastly, the book provides special coverage on utilizing PMF made products to control neglected tropical diseases and plant-made antigens as immunotherapies for allergic and autoimmune diseases.The book has chapters from leading experts on the subject, making it equally beneficial for researchers, policy planners, industrialists, medical professionals, entrepreneurs, and students of various related disciplines.

This edited volume provides state-of¿the-art overview of abiotic stress responses and tolerance mechanisms of different legume crops viz., chickpea, mung bean, lentil, black gram, cowpea, cluster bean, soybean and groundnut.Legumes play an important role in human nutrition and soil health through fixation of nitrogen. Legume production and productivity are vulnerable to different abiotic stresses. A proper understanding about the physiological and molecular basis of the legume crops is essential for genetic improvement of abiotic stress tolerance.  This book consists of 15 chapters covering physiological and biochemical basis, molecular physiology, molecular breeding, genetics, genomics, transgenics, epigenetics of drought, saline, high temperature and nutrient deficiency stresses, and the role of microRNAs in abiotic stress tolerance. This volume offers new perspectives in legume crop abiotic stress management, and is useful for various stakeholders, including post graduates students, scientists, environmentalists and policymakers.

This book explores recent progress in RNA secondary, tertiary structure prediction, and its application from an expansive point of view. Because of advancements in experimental protocols and devices, the integration of new types of data as well as new analysis techniques is necessary, and this volume discusses additional topics that are closely related to RNA structure prediction, such as the detection of structure-disrupting mutations, high-throughput structure analysis, and 3D structure design. Written for the highly successful Methods in Molecular Biology series, chapters feature the kind of detailed implementation advice that leads to quality research results. Authoritative and practical, RNA Structure Prediction serves as a valuable guide for both experimental and computational RNA researchers.

This volume details state-of-the-art computational methods designed to manage, analyze, and generally leverage epigenomic and epitranscriptomic data. Chapters guide readers through fine-mapping and quantification of modifications, visual analytics, imputation methods, supervised analysis, and integrative approaches for single-cell data. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Computational Epigenomics and Epitranscriptomics aims to provide an overview of epiomic protocols, making it easier for researchers to extract impactful biological insight from their data.

Melatonin: A Ubiquitous Pleiotropic Molecule equips researchers with an understanding of the molecular biology of melatonin in plants. The book explores the occurrence of melatonin in several plant species, covering key aspects, including its biosynthesis, physiological function in plant growth and development, and signaling pathways. It then discusses the benefits melatonin has for plant and agricultural development, as well as how it can be utilized as a therapeutic in the treatment of human diseases. The role it can play in the oxidative stress response and circadian rhythm modulation are covered, as well as its nutraceutical potential for human health. The book also covers the importance of melatonin in a range of fields, including drug discovery and disease resistance, agriculture, botany, and biotechnology, and is an ideal reference for researchers across these disciplines.

The Meloscine Core Synthesis Efforts Book, is a comprehensive guidebook on the synthesis of the pentacyclic core of meloscine, an alkaloid with promising biological activities. The book focuses on the expansion of the tetrahydroquinoline pharmacophore, which is an important scaffold for the synthesis of meloscine and its derivatives. The book covers a range of synthetic strategies that can be used to access the pentacyclic core of meloscine. It includes detailed experimental procedures and case studies, making it a valuable resource for organic chemists and researchers working in the field of natural product synthesis. The book also discusses the various challenges and obstacles that arise during the synthesis of meloscine and its derivatives, and provides insight into the strategies that can be used to overcome these challenges. Author writing style is clear and concise, making it easy to understand for both experts and novices in the field of organic chemistry. The book also provides a comprehensive understanding of the pentacyclic core of meloscine, its biological activities, and its potential as a therapeutic agent. In conclusion, the Meloscine Core Synthesis Efforts Book is an excellent resource for organic chemists and researchers interested in the synthesis of natural products. The book provides a comprehensive understanding of the synthetic strategies used to access the pentacyclic core of meloscine and its derivatives, and offers valuable insight into the challenges and obstacles that arise during the synthesis process.

Insect Ecomorphology: Linking Functional Insect Morphology to Ecology and Evolution offers up-to-date knowledge and understanding of the morphology of insects and the functional basis of their diversity. This book covers the form and function of insect body structures in relation to their physiological performance capabilities, biological roles, and evolutionary histories. Written by international experts, the book explores the ecomorphology of functional systems such as insect feeding, locomotion, sensing, and egg laying. The combination of conceptual and review chapters, methodological approaches, and case studies enables readers to delve into active research fields and to gain an understanding of the form-function-performance paradigm. This book uncovers key structures of the various regions of the insect body, elucidates their function, and investigates their ecological and evolutionary implications. Insect Ecomorphology is thus a vital resource for entomologists, biologists, and zoologists, especially those seeking to understand more fully the morphology and physiological impacts of insects in correlation to their environments and to evolution.

Lanthanides in Enzymology and Microbiology, a new volume in the Foundations and Frontiers in Enzymology Series, offers a detailed discussion of lanthanides and lanthanide-dependent enzyme biology. In this book, more than a dozen global experts consider lanthanide enzymology fundamentals, organismal utilization of lanthanides, distribution and diversity of lanthanide-dependent enzymes, regulation of intracellular levels of lanthanides, gene expression regulation via lanthanides, as well as likely applications of lanthanide binding proteins. Lanthanide-dependent methanol and alcohol dehydrogenase metabolism are considered in both methylotrophs and non-methylotrophs, alongside various application areas, from recovery of rare earth elements to developing lanthanide ion binding peptides and biosynthesis of terpolymers through reverse-oxidation pathways. In providing this deep context and pathways for future research, this book acts as an invaluable resource in this emerging field for researchers and students of biochemistry, biotechnology, and environmental microbiology alike.

Neglected Zoonoses and Antimicrobial Resistance: Impact on One Health and Sustainable Development Goals provides a thorough examination of how neglected zoonoses and antimicrobial resistance together hinder the achievement of sustainable development goals declared by the United Nations in the pursuit of a disease-free world. Neglected zoonotic diseases, as defined by the World Health Organization, are diseases likely to impact the livelihoods of livestock keepers and those living in periurban communities in developing countries. This book examines how such zoonoses affect the health of vulnerable farming populations and reduce the production capacity of their livestock. Written by internationally recognized experts in the field of livestock and poultry zoonoses, this book provides the reader with a comprehensive description of modern sustainable development goals and defines neglected zoonotic diseases and their impacts on human physical, mental, social, and economic health. Subsequent chapters systematically describe the epidemiology, hosts and transmission, disease process, economic significance, and prevention and treatment protocols of key neglected zoonotic diseases, including echinococcosis, leishmaniasis, zoonotic tuberculosis, anthrax, brucellosis, leptospirosis, borreliosis, rickettsioses, and rabies. The book concludes with an assessment of the obstacles to achieving Sustainable Development Goals and possible mitigation strategies for veterinary researchers and policymakers alike.

Laccase and Polyphenol Oxidase: Biochemistry and Biotechnological Applications, a new volume in the Foundations and Frontiers in Enzymology series, provides a thorough discussion of the laccase and polyphenol oxidase enzyme families, which play key roles in the oxidation of phenolic substrates and are involved in a range of important biological functions in many organisms. In recent years, significant advances have been made in understanding these enzymes, but few resources exist on their fundamental biology, mechanisms, and applications. In this book, leading world experts discuss the biochemistry of laccase and polyphenol oxidases, research methods, and uses across industries, offering mechanistic and practical overviews of these important enzyme classes and empowering researchers to develop their own studies and hone applications. This book deftly connects topics across these intricate enzyme systems, with early chapters on the physicochemical properties of PPO and laccase, followed by separate sections on these related enzyme classes’ function in biological and industrial systems, production, purification, active assays, and application areas from food and wine to bioremediation to green chemistry.

Personalized Epigenetics, Second Edition discusses the core translatability of epigenetics to health management of individuals who have unique variations in their epigenetic signatures that can guide both disorder and disease prevention and therapy. Fully updated and revised, this new edition details inter-individual variability in the major epigenetic process in humans consisting of DNA methylation, histone modifications, noncoding RNA, and the diagnostic, prognostic, and therapeutic potential of the field. It also reviews the impact of the environment on epigenetic variations among individuals and the role of pharmacology and drug development in personalized epigenetics. Most importantly, the text covers personalized epigenetics from a disease-oriented perspective, presenting chapters that provide advances in widespread disorders or diseases, including diabetes, cancer, autoimmune disorders, obesity, cardiovascular diseases, neurological disorders, and pain management.

This book addresses Epigenetics in Cancer and covers the most recent advances in RNA/histone/DNA epigenetics in Oncology. RNA/histone/DNA epigenetics have been shown to play pivotal roles in cancer initiation, progression, maintenance and drug response/resistance, tumor microenvironment, cancer stem cell self-renewal, cancer metabolism, and tumor immune evasion. In particular, research in RNA cancer epigenetics has made impressive progress in the last few years.Individual chapters in Part I (focusing on RNA epigenetics) are devoted to RNA modifications in Cancer Metabolism and Microenvironment, Cancer Stem Cell Biology, Immune Surveillance, Solid Tumors and Tumor Immunity, and Hematopoietic Malignancies, as well as to RNA editing in Cancer. Chapters in Part II and III of the book focus on histone epigenetics and DNA epigenetics, respectively.By familiarizing readers with the latest developments in this complex and challenging field, the book offers a valuable resourcefor scientists, graduate students and clinicians alike.

This book, entitled Tracking Environmental Change Using Lake Sediments: Volume 6 ¿ Sedimentary DNA, provides an overview of the applications of sedimentary DNA-based approaches to paleolimnological studies. These approaches have shown considerable potential in providing information about the long-term changes of overall biodiversity in lakes and their watersheds in response to natural and anthropogenic changes, as well as tracking human migrations over the last thousands of years.Although the first studies investigating the preservation of these molecular proxies in sediments originate from the late-1990s, the number of scientific publications on this topic has increased greatly over the last five years. Alongside numerous ecological findings, several sedimentary DNA studies have been dedicated to understanding the reliability of this approach to reconstruct past ecosystem changes. Despite the major surge of interest, a comprehensive compilation of sedimentary DNA approaches and applications has yet to be attempted. The overall aim of this DPER volume is to fill this knowledge gap.

Delve into the fascinating world of "Exploring Molecular Biology and Genetic Engineering," a comprehensive guide that takes readers on an illuminating journey through the intricate blueprints and scientific marvels that define life at its most fundamental level.This book offers a clear and engaging exploration of the principles of molecular biology and genetic engineering, providing readers with a profound understanding of the underlying mechanisms shaping life. From the elegant structure of DNA to the intricacies of genetic regulation, every chapter unfolds the mysteries of molecular processes.Readers will navigate the revolutionary advancements in genetic engineering, witnessing how scientists manipulate the building blocks of life to address challenges, enhance medical treatments, and engineer innovative solutions. The narrative blends scientific insights with real-world applications, making complex concepts accessible to both novices and enthusiasts.As the pages unfold, readers will discover the groundbreaking discoveries that have transformed our understanding of genetics. From CRISPR gene editing to the intricacies of recombinant DNA technology, this book bridges the gap between theory and application, revealing the profound impact of molecular biology on medicine, agriculture, and beyond."Exploring Molecular Biology and Genetic Engineering" is more than a textbook; it's a captivating odyssey into the heart of life's machinery. With a perfect balance of scientific rigor and accessibility, this book is an invaluable resource for students, researchers, and anyone seeking to grasp the profound beauty and complexity encoded within the very fabric of our existence. Join this expedition through the blueprints and science of life, and unlock the secrets that shape our biological world.

A DNA computer is a collection of specially selected DNA strands, which when encoded into specific combinations are then subjected to bio-molecular manipulation in order to solve computational problems. Rather than storing information in the 1s and 0s of the binary number system, it is now stored in the form of the bases adenine (A), thymine (T), cytosine (C) and guanine (G). These bases can be arranged into short sequences of DNA that are then artificially synthesised for use as algorithmic inputs. The remarkable advantages of DNA computing, including dense data storage, massively parallel computation, and extraordinary energy efficiency, underscore its potential to revolutionize conventional computing. This innovative approach aligns with a broader trend of harnessing natural processes as computational models.DNA Logic Design: Computing with DNA not only unravels the theoretical intricacies but also navigates the practical challenges, offering a comprehensive exploration of a groundbreaking field at the intersection of biology and computer science. The book starts with the basics of DNA computing, and then describes the fundamental operations of DNA computing. Various kinds of logical designs are then translated into the DNA computing context: arithmetic circuits, combinational circuits, sequential circuits, memory devices, programmable logic devices, and nano processors. Heat and speed calculation techniques round off the book.

Ecophysiology and Ocean Acidification in Marine Mollusks: From Molecule to Behavior provides an extensive overview of the latest research on the various ecophysiological effects of ocean acidification on marine mollusks. This book synthesizes historical information and recent findings on the effects of environmental change, ocean warming, and acidification on key mollusks and their life-history. It also discusses the underlying mechanisms underpinning the effects of ocean warming and acidification. Written by internationally recognized experts in the field of marine biology, this book systematically examines the effects of ocean acidification on the reproduction, growth and development, physiological metabolism, immunity, and behavior of marine mollusks. The book concludes by discussing the implications of current research, acknowledging data limitations in the field, and proposing future research directions, providing a better understanding of the potential impacts of ocean acidification on mollusks and the global aquaculture industry and inspiring new thinking for future research practices. It will be an indispensable resource for researchers, practitioners, undergraduate and graduate students, conservationists, and aquaculturists alike who are interested in marine environmental change, ecology, physiology, and marine biology.

Nature-Inspired Sensors presents and discusses the basic principles and latest developments in nature-inspired sensing and biosensing materials as well as the design and mechanisms for analyzing their potential in multifunctional sensing applications. The book starts with a comprehensive review of certain fundamental mechanisms in different living creatures, including humans, animals, and plants. It presents and discusses ways for imitating various nature-inspired structural features and their functional properties, such as hierarchical, interlocked, porous, and bristle-like structures and hetero-layered brick-and-mortar structures. It also highlights the utility of these structures and their properties for sensing functions, which include static coloration, self-cleaning, adhesive, underwater navigation and object detection, electric charge generation, and sensitive olfactory functions for detecting various substances. This is followed by an appraisal of accumulating knowledge and its translation from the laboratory to the point-of-care phase, using selective sensors as well as desktop and wearable artificial sensing devices, for example, electronic noses and electronic skins, in conjunction with AI-assisted data processing and decision-making in the targeted field of application. In addition, the book offers an insight into the challenges of continuing the development of nature-inspired smart sensing and biosensing technology and their wider availability, which can be substantially improved. It is a valuable reference for graduates, undergraduates, researchers, and working professionals in the fields of chemistry, materials science, and biomedical and environmental science.

Advanced Biophysical Techniques for Polysaccharides Characterization offers detailed insights into the cutting-edge techniques available for the identification, quantification, characterization. and structural analysis of polysaccharides. A wide range of techniques are covered, including scanning electron microscopy (SEM), atomic force microscopy (AFM), optical microscopy, non-linear optical microscopy and spectroscopic techniques like Fourier transform Infrared (FTIR), X ray diffraction, light scattering, and nuclear magnetic resonance (NMR). Various polysaccharides are investigated, along with their applications across a range of industries. Each chapter offers a detailed description of the techniques before delving into case studies covering the latest advances. This book provides a one-stop solution to the latest advanced microscopic and spectroscopic techniques for investigating a range of important polysaccharides and is an ideal reference for researchers in the field of biophysics, molecular biology, biochemistry, pharmaceutics, food chemistry and related areas.