Cellebiologi (cytologi)

This volume details a wide range of methods, ranging from beginner through advanced, used tofurther study zebrafish and fish germline.. Chapter guide readers through cultivating and manipulating germ cells, imaging of germline processes and the molecular analysis of their, protein, and RNA. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Germline Development in the Zebrafish: Methods and Protocols aims to ensure successful results in the further study of this vital field.

This volume provides a comprehensive review of concepts and protocols related to fluorescence in situ hybridization (FISH) applied to microbial cells. Chapters will serve guide for the design of new probes and the development of novel FISH-based protocols. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Fluorescence In Situ Hybridization (FISH) for Microbial Cells: Methods and Concepts aims to ensure successful results in the further study of this vital field.

This volume explores the techniques used to study human and animal pericytes. Chapters in this volume cover a range of topics such as pericyte dissociation from tissues, and analysis by immunohistochemistry and flow cytometry; avian chimaeras to investigate the embryonic development of cell lineage; pericyte purification by fluorescence activated cell sorting and ensuing culture; tissue turnover and regeneration; and the dynamic study of pericyte involvement in cancer dissemination, in situ, and in culture. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Pericytes: Methods and Protocols is a valuable resource that helps researchers define the biologic identity, functions, and potentials ofa cell lineage of increasing interest and therapeutic significance.

This second edition of Eosinophils: Methods and Protocols updates several techniques from the first edition together with novel in vitro and in vivo-based methodologies. Written by internationally recognized expert authorities, this volume provides vital techniques from eosinophil purification to experimental modelling, with each technique spelled out in clear and straightforward terms, assuming no previous knowledge of the method and without necessitating sourcing additional information elsewhere. Written in the highly successful Methods in Molecular Biology format, chapters include introductions to their respective topics, lists of the essential materials and reagents, step-by-step, readily reproducible laboratory protocols, with tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Eosinophils: Methods and Protocols, Second Edition provides the practical means to extend our knowledge of eosinophil function in health and disease, underpinning research that may in turn lead to new hypotheses for future examinations into the role of this intriguing and enigmatic leukocyte.Chapters 10 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

This detailed book examines experimental approaches used to investigate the regulation and function of phosphoinositides (PtdInsP), rare eukaryotic phospholipids with a broad role in biological processes such as signal transduction, cell migration and adhesion, cell growth, subcellular organization, and membrane trafficking. The combination of complementary biochemical, mass spectrometry, and imaging methods are instrumental for the detection and quantification of PtdInsP species, as well as induced dimerization methods, affinity precipitation or co-sedimentation with liposomes, protein insertion within lipid bilayers, and enzymatic assays, or through emerging methods like native mass spectrometry and microfluidics, all of which are touched upon in this volume. Written in the highly successful Methods in Molecular Biology format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratoryprotocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Phosphoinositides: Methods and Protocols provides detailed methodology for both specialist and novice researchers on a variety of complementary methods that have been instrumental in dissecting the regulation, dynamics, and function of PtdInsPs.

This detailed book collects a variety of methods from investigators at the forefront of chondrocyte biology and pathology research that they have tailored for their research projects in order to tackle the unique properties and challenges of the chondrocyte and those of its tissue matrix. Divided into three parts, the volume explores experimental models to study chondrocytes, in vivo assays, as well as methods to characterize the chondrocyte phenotype with high-throughput or discrete assays on cells maintained in vivo, just isolated from their tissue (ex vivo), or cultured in vitro. Written in the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Chondrocytes: Methods andProtocols is an ideal guide for experts as well as newcomers in the cartilage field with the aim of sparking new research ideas in order to solve many of the pending mysteries regarding cartilage biology, disease mechanisms, and potential treatments.

This book presents recent scientific investigations in microbial ecology and systematics. Current research on microorganisms is presented as a perfect book for studies on "Microbial Systematics". This book will serve as an important resource for practising research and review for the scientific community.

This book, Clinical Biochemistry and Drug Development: From Fundamentals to Output, focuses on clinical biochemistry fundamentals, cell culture techniques, and drug discovery and development concepts. It deals with three different fields of clinical research: cell culture, clinical biochemistry, and drug discovery and development.

This detailed collection gathers both established and recent technical procedures to study the Endosomal Sorting Complex Required for Transport (ESCRT) complexes in a wide range of biological systems: Archaea, A. thaliana, U. maydis, S. cerevisiae, S. pombe, C. elegans, D. melanogaster, and mammalian cells. Opening with a section on imaging techniques, the book continues with chapters covering biochemical approaches presenting strategies for production and characterization of recombinant ESCRT proteins, or of specific ESCRT protein domains, as well as genetic and proteomic experimental approaches. Written for the highly successful Methods in Molecular Biology series, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical,The ESCRT Complexes: Methods and Protocols serves as a compact guide for researchers interested in establishing an integrated approach to investigate the ESCRT machinery functions in cell biology.

This book aims at providing insights into the collagen superfamily and the remarkable diversity of collagen function within the extracellular matrix. Additionally, the mechanisms underlying collagen-related diseases such as dystrophic epidermolysis bullosa, osteogenesis imperfecta, as well as collagen-related myopathies and neurological disorders are discussed.Collagens are the most abundant extracellular matrix proteins in organisms. Their primary function is to provide structural support and strength to cells and to maintain biomechanical integrity of tissues. However, collagens can no longer be considered just as structural proteins. They can act as extracellular modulators of signaling events and serve critical regulatory roles in various cell functions during embryonic development and adult homeostasis. Furthermore, collagens are associated with a broad spectrum of heritability-related diseases known as ¿collagenopathies¿ that affect a multitude of organs and tissues including sensorial organs.The book is a useful introduction to the field for junior scientists, interested in extracellular matrix research. It is also an interesting read for advanced scientists and clinicians working on collagens and collagenopathies, giving them a broader view of the field beyond their area of specialization.

From a lab in the Sahara, where one problem is sand in the petri dishes, to an Israeli lab that narrowly escapes a terrorist bomb, stem cells have gone global. Not only are the cells studied in an escalating number of labs-and lands-but they are already being used. In Japan, a respected doctor uses the cells to make small women better endowed. In Connecticut, stem cell technology has created cloned cows that roam the hills displaying eerily identical personalities. In Texas, stem cells rejuvenate dying hearts. In China, clinics offer stem cells to patients suffering from everything from paralysis to brain trauma.In elegant, cogent prose, science journalist Cynthia Fox has illuminated the reality and promise of stem cell therapies. Cell of Cells illustrates how the extensive, fervent experimentation currently under way is causing a revolution, both in the body and in the international body politic.

Approx.264 pages

This volume of the Encyclopedia of Complexity and Systems Science, Second Edition, focuses on current challenges in the field from materials and mechanics to applications of statistical and nonlinear physics in the life sciences. Challenges today are mostly in the realm of non-equilibrium systems, although certain equilibrium systems also present serious hurdles. Where possible, pairwise articles focus on a single topic, one from a theoretical perspective and the other from an experimental one, providing valuable insights. In other cases, theorists and experimentalists have collaborated on a single article. Coverage includes both quantum and classical systems, and emphasizes 1) mature fields that are not covered in the current specialist literature, (2) topics that fall through the cracks in disciplinary journals/books, or (3) developing areas where the knowledge base is large and robust and upon which future developments will depend. The result is an invaluable resource for condensed matter physicists, material scientists, engineers and life scientists.