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The overall sequence is as follows: Genome structure, Gene Structure and Expression, Nucleolar Genes, Structure and Proteins, Chromatin and Nuclear Granules, Nuclear Matrix and Nuclear Proteins, Replication and Transcription and finally Nuclear Envelope and Nuclear Cytoplasmic Transport.
This work provides detailed practical methods for specimen preparation for negative staining and cryoelectron microscopy of thinly spread samples of biological particulates. The different possibilities are emphasized and the benefits to be gained by the various techniques clearly explained.
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