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Aparajita is a tenacious go-getter. Her name means unconquerable in Sanskrit, and she lives up to its meaning. Just like any other ambitious girl, she desires to fulfil her dreams and become an independent individual. Far and wide, the shadow of her melancholy past chases her passage. The fact that her widowed mother is a former sex worker irks the community. Nonetheless, she is not ashamed to reveal her mother's past. Will she lose hope, or will she defy an enigma that is centuries-old? Will she ever conquer the hearts of a prestige-obsessed community? See the world through Aparajita's prism in a tale stirred by some real life events.
Feelings. Emotions. Thoughts.This book has been written with honesty and sincerity, every thought, every sentence of the proses printed in this book is something that the author has witnessed, felt, imbibed in his journey. Heartbreaks, waiting for someone, dreaming about someone, missing, wanting to touch, wanting to be with someone. The book is all about love, friendship, situations and more. The book is simply out of everyday feeling and thoughts of the author.
This volume brings together cutting-edge laboratory protocols to characterize the novel fluorescent proteins (FPs) and approaches based on fluorescent proteins that aim to answer some of the key cell biological questions. The book covers topics ranging from the database of fluorescent proteins to their characterization and adaptation to a wide range of biological systems. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Fluorescent Proteins: Methods and Protocols serves as an ideal guide for students and academicians enthusiastic about the recent progress in the practical application of fluorescent protein technology.
GET OUT OF YOUR PROGRAMMED LIFEHumans, no doubt, are highly advanced and incredible machines. Like any other machine, human beings also work on the programmes downloaded in the mind. Unfortunately, most of us are being run and controlled by somebody else's programmes. The principles laid down in this book are based on the research of modern science, and you may use them to improve your programmed life. You are what you are programmed for. By understanding how your brain is designed and how it works, you can control your programming and improve the quality of your life. Ultimately, the quality of programmes loaded in your mind runs your life. You need to know how your mind works, how you are programmed and how you can get rid of incorrect and faulty programmes. We have all the powers within us to live a happy, healthy and successful life. However, you need to learn to programme yourself with the correct programmes. Your brain works like a computer, so make sure that you are the only one who programmes it.
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