Bøger af Shabir Hussain Wani

Maize is one of the most generally grown cereal crops at global level, followed by wheat and rice. Maize is the major crop in China both in terms of yield and acreage. In 2012, worldwide maize production was about 840 million tons. Maize has long been a staple food of most of the global population (particularly in South America and Africa) and a key nutrient resource for animal feed and for food industrial materials. Maize belts vary from the latitude 58° north to the latitude 40° south, and maize ripens every month of the year. Abiotic and biotic stresses are common in maize belts worldwide. Abiotic stresses (chiefly drought, salinity, and extreme temperatures), together with biotic stresses (primarily fungi, viruses, and pests), negatively affect maize growth, development, production and productivity. In the recent past, intense droughts, waterlogging, and extreme temperatures have relentlessly affected maize growth and yield. In China, 60% of the maize planting area is prone to drought, and the resultant yield loss is 20%-30% per year; in India, 25%-30% of the maize yield is lost as a result of waterlogging each year. The biotic stresses on maize are chiefly pathogens (fungal, bacterial, and viral), and the consequential syndromes, like ear/stalk rot, rough dwarf disease, and northern leaf blight, are widespread and result in grave damage. Roughly 10% of the global maize yield is lost each year as a result of biotic stresses. For example, the European corn borer [ECB, Ostrinianubilalis (Hübner)] causes yield losses of up to 2000 million dollars annually in the USA alone in the northern regions of China, the maize yield loss reaches 50% during years when maize badly affected by northern leaf blight. In addition, abiotic and biotic stresses time and again are present at the same time and rigorously influence maize production. To fulfill requirements of each maize-growing situation and to tackle the above mentions stresses in an effective way sensibly designed multidisciplinary strategy for developing suitable varieties for each of these stresses has been attempted during the last decade. Genomics is a field of supreme significance for elucidating the genetic architecture of complex quantitative traits and characterizing germplasm collections to achieve precise and specific manipulation of desirable alleles/genes. Advances in genotyping technologies and high throughput phenomics approaches have resulted in accelerated crop improvement like genomic selection, speed breeding, particularly in maize. Molecular breeding tools like collaborating all omics, has led to the development of maize genotypes having higher yields, improved quality and resilience to biotic and abiotic stresses. Through this book, we bring into one volume the various important aspects of maize improvement and the recent technological advances in development of maize genotypes with high yield, high quality and resilience to biotic and abiotic stresses

Soybean (Glycine max L. (Merr)) is one of the most important crops worldwide. Soybean seeds are vital for both protein meal and vegetable oil. Soybean was domesticated in China, and since last 4-5 decades it has become one of the most widely grown crops around the globe. The crop is grown on an anticipated 6% of the world's arable land, and since the 1970s, the area in soybean production has the highest percentage increase compared to any other major crop. It is a major crop in the United States, Brazil, China and Argentina and important in many other countries. The cultivated soybean has one wild annual relative, G. soja, and 23 wild perennial relatives. Soybean has spread to many Asian countries two to three thousand years ago, but was not known in the West until the 18th century. Among the various constraints responsible for decrease in soybean yields are the biotic and abiotic stresses which have recently increased as a result of changing climatic scenarios at global level. A lot of work has been done for cultivar development and germplasm enhancement through conventional plant breeding. This has resulted in development of numerous high yielding and climate resilient soybean varieties. Despite of this development, plant breeding is long-term by nature, resource dependent and climate dependent. Due to the advancement in genomics and phenomics, significant insights have been gained in the identification of genes for yield improvement, tolerance to biotic and abiotic stress and increased quality parameters in soybean. Molecular breeding has become routine and with the advent of next generation sequencing technologies resulting in SNP based molecular markers, soybean improvement has taken a new dimension and resulted in mapping of genes for various traits that include disease resistance, insect resistance, high oil content and improved yield.This book includes chapters from renowned potential soybean scientists to discuss the latest updates on soybean molecular and genetic perspectives to elucidate the complex mechanisms to develop biotic and abiotic stress resilience in soybean. Recent studies on the improvement of oil quality and yield in soybean have also been incorporated.

With the erratic changes in climate, crop plants are facing many forms of biotic stresses. When plants are under stress, among several gene families, regulatory genes play a vital role in signal transduction in modulating the expression of genes underpinning several defense pathways and targeting regulatory proteins (viz, transcription factors (TFs)) can be the alternative. Transcription factors directly regulate the downstream R genes and are excellent candidates for disease resistance breeding.  Till date, numerous transcription factors have been identified and characterized structurally and functionally. Of them, TF families such as WRKY, NAC, Whirly, Apetala2 (AP2), ethylene responsive elements (ERF) etc. are found to be associated with transcriptional reprogramming of plant defense response. These TFs are responsive to the pathogen's PAMPs/DAMPs - host's PRR protein interactions and specifically binds to the cis-elements of defense genes and regulate their expression. With this background, realizing the importance of TFs in resistance breeding, this book discusses the recent research and developments in this field for various crops.

Maize is one of the most generally grown cereal crops at global level, followed by wheat and rice. Maize is the major crop in China both in terms of yield and acreage. In 2012, worldwide maize production was about 840 million tons. Maize has long been a staple food of most of the global population (particularly in South America and Africa) and a key nutrient resource for animal feed and for food industrial materials. Maize belts vary from the latitude 58° north to the latitude 40° south, and maize ripens every month of the year. Abiotic and biotic stresses are common in maize belts worldwide. Abiotic stresses (chiefly drought, salinity, and extreme temperatures), together with biotic stresses (primarily fungi, viruses, and pests), negatively affect maize growth, development, production and productivity. In the recent past, intense droughts, waterlogging, and extreme temperatures have relentlessly affected maize growth and yield. In China, 60% of the maize planting area is prone to drought, and the resultant yield loss is 20%¿30% per year; in India, 25%¿30% of the maize yield is lost as a result of waterlogging each year. The biotic stresses on maize are chiefly pathogens (fungal, bacterial, and viral), and the consequential syndromes, like ear/stalk rot, rough dwarf disease, and northern leaf blight, are widespread and result in grave damage. Roughly 10% of the global maize yield is lost each year as a result of biotic stresses. For example, the European corn borer [ECB, Ostrinianubilalis (Hübner)] causes yield losses of up to 2000 million dollars annually in the USA alone in the northern regions of China, the maize yield loss reaches 50% during years when maize badly affected by northern leaf blight. In addition, abiotic and biotic stresses time and again are present at the same time and rigorously influence maize production. To fulfill requirements of each maize-growing situation and to tackle the above mentions stresses in an effective way sensibly designed multidisciplinary strategy for developing suitable varieties for each of these stresses has been attempted during the last decade. Genomics is a field of supreme significance for elucidating the genetic architecture of complex quantitative traits and characterizing germplasm collections to achieve precise and specific manipulation of desirable alleles/genes. Advances in genotyping technologies and high throughput phenomics approaches have resulted in accelerated crop improvement like genomic selection, speed breeding, particularly in maize. Molecular breeding tools like collaborating all omics, has led to the development of maize genotypes having higher yields, improved quality and resilience to biotic and abiotic stresses. Through this book, we bring into one volume the various important aspects of maize improvement and the recent technological advances in development of maize genotypes with high yield, high quality and resilience to biotic and abiotic stresses

Over the last few decades, various techniques have been developed to alter the properties of plants and animals.  While the targeted transfer of recombinant DNA into crop plants remains a valuable tool to achieve a desirable breeding outcome, integration of transgenes into the host genome has been random, which in part, leads to reduced acceptance of GMOs by the general population in some parts of the world. Likewise, methods of induced mutagenesis, such as TILLING, have the disadvantage that many mutations are induced per plant, which has to be removed again by expensive backcrossing. Advances in genome sequencing have provided more and more information on differences between susceptible and resistant varieties, which can now be directly targeted and modified using CRISPR/Cas9 technology. By selecting specific gRNAs occurrence of off-target modifications are comparatively low. ZFNs and TALENs- based approaches required re-engineering a new set of assembled polypeptides for every new target site for each experiment. The difficulty in cloning and protein engineering prevented these tools from being broadly adopted by the scientific community.  Compared to these technologies, designing the CRISPR toolbox is much simpler and more flexible. CRISPR/Cas9 is versatile, less expensive and highly efficient. It has become the most widely used technology for genome editing in many organisms.Since its inception as a powerful genome-editing tool in late 2012, this breakthrough technology has completely changed how science is performed. The first few chapters in this book introduce the basic concept, design and implementation of CRISPR/Cas9 for different plant systems. They are followed by in-depth discussions on the legal and bio-safety issues accompanying commercialization and patenting of this emerging technology. Lastly, this book covers emerging areas of new tools and potential applications. We believe readers, novice and expert alike, will benefit from this all-in-one resource on genome editing for crop improvement.Chapter 17 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Plants, being sessile and autotrophic in nature, must cope with challenging environmental aberrations and therefore have evolved various responsive or defensive mechanisms including stress sensing mechanisms, antioxidant system, signaling pathways, secondary metabolites biosynthesis, and other defensive pathways among which accumulation of osmolytes or osmo-protectants is an important phenomenon. Osmolytes with organic chemical nature termed as compatible solutes are highly soluble compounds with no net charge at physiological pH and nontoxic at higher concentrations to plant cells. Compatible solutes in plants involve compounds like proline, glycine betaine, polyamines, trehalose, raffinose family oligosaccharides, fructans, gamma aminobutyric acid (GABA), and sugar alcohols playing structural, physiological, biochemical, and signaling roles during normal plant growth and development. The current and sustaining problems of climate change and increasing world population has challenged global food security. To feed more than 9 billion, the estimated population by 2050, the yield of major crops needs to be increased 1.1-1.3% per year, which is mainly restricted by the yield ceiling. A major factor limiting the crop yield is the changing global environmental conditions which includes drought, salinity and extreme temperatures  and are responsible for a reduction of crop yield in almost all the crop plants. This condition may worsen with a decrease in agricultural land or the loss of potential crop yields by 70%. Therefore, it is a challenging task for agricultural scientists to develop tolerant/resistant varieties against abiotic stresses. The development of stress tolerant plant varieties through conventional breeding is very slow due to complex multigene traits. Engineering compatible solutes biosynthesis by deciphering the mechanism behind the abiotic tolerance or accumulation in plants cell is a potential emerging strategy to mitigate adverse effects of abiotic stresses and increase global crop production. However, detailed information on compatible solutes, including their sensing/signaling, biosynthesis, regulatory components, underlying biochemical mechanisms, crosstalk with other signaling pathways, and transgenic development have not been compiled into a single resource. Our book intends to fill this unmet need, with insight from recent advances in compatible solutes research on agriculturally important crop plants.

This volume details different genomic methods and resources to explore cereal genomics. Chapters guide readers through crop genomes, Next Generation Sequencing (NGS) technologies, protocol for CRISPR editing, transgenic wheat, NGS approach, virus induced gene silencing (VIGS), genomic tools, computational prediction of ncRNAs (miRNAs & ceRNAs) in cereal crops, genotyping-by-sequencing (GBS), Bayesian method, single cell sequencing, genome-wide association study (GWAS), QTL interval mapping, whole genome bisulfite sequencing, genome imprinting, and methods for study the receptor-metabolite interaction.   Authoritative and cutting-edge, Genomics of Cereal Crops aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.