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The present study was planned to develop antibacterial treatment on cotton fabric by using lemon peel extract. To achieve the objectives proposed, lemon peel extract was selected for treatment application on cotton fabric. Pretreatment was given to cotton fabric and treatment was applied on cotton fabric in standardized concentration of lemon peel extract with exhaust and pad dry cure method. Lemon peels were extracted by cold aqueous maceration. It was found that percentage yield of extract was 19.6% for lemon peel extract. Bacillus spp. were selected for the present study. To study the effect of lemon peel extract the treated samples were tested for microbial resistance actively quantitatively by AATCC-100 test method before and after washing of samples. Treated fabrics were tested for physical properties and found that fabric weight, thickness and bulk of fabric were increased while tensile strength, elongation, bending length, flexural rigidity, air permeability and moisture regain were decreased. After washing physical properties of treated fabric were investigated and found that fabric count, weight, thickness bulk and bending length of lemon peel extract.
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