Bøger udgivet af Springer US

This protocol book presents reproducible and step-by-step procedures for the peptide synthesis, their characterization and applications. The volume includes an introductory section on in silico modelling of new peptide molecules, Molecular Dynamics Simulations, Docking, Electrostatic fingerprinting of peptides, and other modelling tools for peptide designing and optimization. Further, it covers protocols for the solid phase peptide synthesis, chromatographic and mass spectrometric characterization of peptides. Importantly, it covers methods for biophysical characterizations of peptides for their potential applications as drug delivery vehicles, peptide nano-assembly, bionanocatalysis, protein aggregation diseases, and peptide-based anti-bacterial.¿

This fully updated edition introduces new tools, models, and analytic insights that position the zebrafish even more strongly as an engine of discovery for developmental and disease biology. Beginning with a section exploring detailed methods for use of zebrafish to model a variety of human diseases, the book continues by illuminating the key ongoing role of the fish model in studies of the vertebrate nervous system, tools and approaches using zebrafish to study stem cell and regenerative biology, as well as techniques in genetics and genomics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Zebrafish: Methods and Protocols, Third Edition serves as an invaluable guide to propel advances in developmental biology, disease modeling, and regeneration research using zebrafish and medaka as model systems.

This volume presents both theoretical guidance and protocols on chemogenomics including chemogenomics library assembly, compound profiling, and phenotypic assays. The chapters in this book cover topics such as the assembly and use of Kinase Chemogenomics; data mining for chemogenomic compound candidates; protocols for protein family-focused assay systems to profile chemogenomic compounds; functional and target engagement assays in cellular settings for broad characterization; and a discussion on phenotypic assays where chemogenomic sets may be applied. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and thorough, Chemogenomics: Methods and Protocols is a valuable resource for all researchers whoare interested in learning more about this diverse and developing field.

This detailed volume presents status and management protocols with the objective of identifying proper guidelines and materials for research related to the most common malignant neoplasm in the thyroid gland, papillary thyroid carcinoma. After an introduction, the book explores diagnostic approaches and initial surgical managements for patients with papillary thyroid carcinoma, guidelines for macroscopic examination, microscopic examination, and pathological staging, molecular approaches to this cancer, pathology laboratory approaches, as well as radiology oncology treatment protocols. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols that are readily reproducible, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Papillary Thyroid Carcinoma: Methods and Protocols serves as a vital resource for information on different aspects of the processes, cost, and resources available for the research and management of patients with papillary thyroid carcinoma.

This collection explores the latest advances in riboregulators, such as RNA-only systems and ribonucleoprotein systems, and provides detailed techniques to study, evolve, and design them. Beginning with a set of chapters focused on the design and application of small RNA (sRNA) regulator systems, the book continues with sections on techniques to create switchable riboregulator systems known as riboswitches, technologies that leverage RNA-guided CRISPR-Cas systems to edit the epigenome, control gene expression, and create diagnostics, as well as computational and experimental techniques to investigate the sequence-structure-function relationship of RNA systems that can both advance fundamental understanding and rational design of riboregulators. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, andtips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Riboregulator Design and Analysis is an ideal guide for scientists and engineers interested in the design and application of riboregulators and driving further innovation in the field.

Recent developments in lesion-symptom mapping (LSM) have spurred rapid growth. This volume provides comprehensive coverage of the steps and considerations involved in LSM. The chapters cover the definition and types of brain lesions, how to prepare them for analysis, standard LSM methods, network-based LSM methods, and approaches of transient lesions induced by brain stimulation. These chapters are supplemented by practical, hands-on mini tutorials on implementing the different analyses using freely-available software. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get started using LSM in your laboratory. Cutting-edge and thorough, Lesion-to-Symptom Mapping: Principles and Tools connects core conceptual issues with available tools, making it a valuable resource for experienced and new researchers.

This detailed volume explores the large research area of Notch signaling studies in a variety of model organisms. The collected research methods and protocols include the dissection of Notch functional sites, Notch regulators, Notch activity reporters and analysis, roles of Notch in development and diseases, wet and dry lab tools and studies, and bioinformatics analysis. This book particularly emphasizes on the exploration of Notch roles in development and diseases, while acknowledging the importance of combining classic and novel molecular tools and methods, bioinformatics workflows and applications, and different model organisms to fully understand the subject area. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Notch Signaling Research: Methods and Protocols is an ideal guide for researchers in the field, with techniques that could potentially be applied to study the wider world of other signaling pathways as well.

This volume covers various aspects of affinity chromatography along with examples of its applications. Chapters guide readers through methodologies to purify a diverse array of molecular targets such as antibodies, extracellular vesicles, recombinant proteins, biomarkers, metabolites, plant organelles, nucleic acids, ligand identification and protocols on building affinity matrix. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Affinity Chromatography: Methods and Protocols aims to be an invaluable resource to anyone employing affinity chromatography-based methodologies.

This volume presents current methods to detect and measure the activity of proteolytic enzymes in organisms ranging from unicellular algae to flowering plants. Chapters detail in vitro production, characterization of plant proteases, tools for in vivo modifications, proteomic approaches for identification of substrates, inhibitors and interacting partners, and the function of plant proteases in plant programmed cell death. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Plant Proteases: Methods and Protocols aims to be a useful practical guide to researches to help further their study in this field.

This volume provides a compilation of methods that will aid researchers studying virology, immunology, and vaccinology of this devastating disease of swine. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.Authoritative and cutting-edge, African Swine Fever Virus: Methods and Protocols aims to be a foundation for future studies and to be a source of inspiration for new investigations in the field.

This volume explores a collection of methods that studies genome editing across a variety of bacteria, phages, and plants. Chapters in this book cover topics such as scarless DNA recombineering of phage in the lysogenic state; HEMSE; Dup-In and DIRex; recombineering in Staphylococcus aureus; and genome editing with Cas9 in lactobacilli. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and thorough, Recombineering: Methods and Protocols is a valuable resource for any researchers interested in learning more about this developing field.

This volume looks at the latest methods used to study and modulate the biological function and mechanisms of SH2 domains. The chapters in this book are organized into five parts. Part One presents methodology aimed at determining the structures and dynamics of SH2 domains and their complexes with phosphopeptides. Part Two discusses techniques to understand and predict interactions of SH2 domains by measuring or calculating their affinity to phosphopeptides. Part Three focuses on inhibitors of SH2 domains that lead the way for chemical tool development and drug discovery. Part Four describes how to evolve and engineer SH2 domains with specific binding properties, and Part Five explores how to measure the regulation of protein tyrosine phosphatase activity through allosteric binding of peptides to SH2 domains and condensation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and comprehensive, SH2 Domains: Functional Modules and Evolving Tools in Biology is a valuable resource for researchers, working in the biophysical and biochemical field, who want to learn more about this exciting and versatile class of regulatory and signaling domains.

This volume discusses the latest online plant genomics and cytogenetic resources used by plant evolutionary biologists and plant breeders. The chapters in this book are organized into two parts. Part One looks at plant genomic databases, and covers topics such as plant phenomics and genomics research data repositories, InpactorDB, PlanTEenrichment, and PEATmoss, among others. Part Two looks at cytogenetics and chromosome-related databases, and covers resources such as the Plant DNA C-values database, the Delphineae Chromosome Database (DCDB), B-chrom, a Database on B-chromosomes, and the Plant Ribosomal DNA Database. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective databases and offers explicit directions on how to access and get the most of these resources. Cutting-edge and comprehensive, Plant Genomic and Cytogenetic Databases is a valuable instrument for any plant science researcher who is interested in learning more about the wealth of information that is available through the use of these databases.

This detailed volume explores techniques to study reactive oxygen species (ROS) in plants and to characterize their roles in development and stress responses. Beginning with a section on strategies to induce ROS production, the book continues with methods to visualize ROS and detect changes in redox homeostasis, small-scale and targeted analyses for investigating the effects of ROS accumulation during stress on plant physiology and metabolism, as well as systems biology approaches to understand ROS functions. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Reactive Oxygen Species in Plants: Methods and Protocols serves as a vital resource that any researcher, and in particular young researchers, can use and adapt to further our knowledge of this dynamic area of plant science.

This detailed volume explores experimental laboratory procedures for a wide range of steroid bioconversions. After an overview on the current trends and perspectives, the book continues with sections covering microbial screening and synthetic biology applied to microorganisms able to catabolize sterols, methods on strain characterization, including omics and biochemical analyses, methods of fermentation and biocatalysis for steroids production, as well as a chapter on the medical use of glucocorticoids in cancer patients. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Microbial Steroids: Methods and Protocols, Second Edition serves as an ideal reference source for laboratory and industrial professionals, as well as for students in a wide array of biological disciplines.

This volume discusses the latest mass spectrometry (MS)-based technologies for proteoform identification, characterization, and quantification. Some of the topics covered in this book include sample preparation, proteoform separation, proteoform gas-phase fragmentation, and bioinformatics tools for MS data analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and comprehensive, Proteoform Identification: Methods and Protocols is a valuable resource for researchers in both academia and the biopharmaceutical industry who are interested in proteoform analysis using MS.

This volume looks at the framework in which different scientific disciplines are integrated into the latest studies in behavioral neurogenetics, and covers new approaches toward understanding the system that controls behavior across the animal kingdom. Chapters in this book cover topics such as mapping brain-wide mesoscale connectome from single animals with BRICseq; gut microbes and Drosophila behavior; bioassaying the function of pheromones in Drosophila melanogaster¿s social behavior; automated behavior analysis using a YOLO-based object detection system; and proteomic analysis of C. elegans neurons using TurboID-based proximity labeling. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Cutting-edge and practical, Behavioral Neurogenetics is a valuable resource for experienced and novice researchers interested in learning more about this field and its future developments.

This detailed volume provides the increasing number of SARS-CoV-2 researchers with a useful handbook covering multidisciplinary approaches on various aspects of SARS-CoV-2 research, brought together by leading laboratories across the globe. Topics covered include techniques in clinical and diagnostic virology, basic protocols in cell and virus culture, as well as bioinformatics and proteomics approaches in cellular response studies. This comprehensive collection also covers methods in immunology, animal models, antivirals and vaccine development strategies, as well as biorisk and mitigation measurements for SARS-CoV-2 research. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of detailed implementation advice that is vital for success in the lab. Practical and timely, SARS-CoV-2: Methods and Protocols serves as an ideal guide for scientists investigating this prevalent and perilous RNA virus and the novel coronavirus disease that results from it.

This second edition details new and updated methods on different antibody libraries, along with novel approaches for antibody discovery. Chapters focuses on the construction of antibody libraries, antibody expression, complementary approaches for antibody selection, and other phage display related applications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Phage Display: Methods and Protocols, Second Edition aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.

This third edition expands on the previous editions with updated and new chapters on protein chromatography. Chapters detail protein stability and storage, avoiding proteolysis, protein quantitation methods, generation and purification of recombinant proteins, recombinant antibody production, and the tagging of proteins. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Protein Chromatography: Methods and Protocols, Third Edition aims to provide commonly used methods and new approaches to help both new researchers and experts expand their knowledge.

This detailed volume provides a comprehensive set of experimental protocols and useful strategies to examine the repair of damaged bases via the Base Excision Repair (BER) pathway in vitro and in cells. Beginning with multiple molecular and cellular techniques to examine the excision of damaged bases from double-stranded DNA or DNA wrapped in a nucleosome, the book continues with sections covering procedures to detect and quantify the damaged bases, protein DNA crosslinks, and double-strand breaks, experimental procedures to identify DNA repair protein interactome by conventional tandem affinity purification followed by mass spectroscopy analysis, as well as the analysis of genome-wide binding of DNA repair proteins and copy number variations of the DNA damage response gene in tumors. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topis, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Base Excision Repair Pathway: Methods and Protocols serves as a valuable resource for novices and experts trying to examine the repair of various types of DNA lesions in vitro and in cell by the distinct set of proteins in the BER pathway.

This third edition volume expands on the previous editions with new discussions on the latest techniques and developments in the field. The chapters in this book are organized into four parts, and cover topics such as optical tweezers; single-molecule fluorescence tools; atomic force microscopy; magnetic tweezers; applications to virus protein shells, unfolding of proteins, nucleic acids, motor proteins, in vivo and in vitro; and protocols to establish specific surface interactions and perform force calibration. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and thorough, Single Molecule Analysis: Methods and Protocols, Third Edition is a valuable resource for all researchers who wantto learn more about this exciting and still expanding field.Chapters 2, 7, 8, 9, 12, 18, and 19 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

This second edition details protocols that analyze and explore gene regulatory networks (GRNs). Chapters guide readers through experimental techniques used to study genes and their regulatory interactions in plants, and computational approaches used for the integration of experimental data and bioinformatics-based predictions of regulatory interactions. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Plant Gene Regulatory Networks: Methods and Protocols, Second Edition aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.

This second volume focuses on PCR methods and PCR application specificities to the biotechnology and bioengineering field. New and updated chapters detail real-time PCR protocols, synthetic biology applications, pathogen detection, microfluidics, digital, multiplex detection recent advances. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, PCR: Methods and Protocols, Second Edition aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.

This detailed book explores new molecular biological techniques for the detection of both antigens and antibodies of porcine diseases. Assays examined within this collection include improved methods of nucleic acid extraction, polymerase chain reaction (PCR), droplet digital PCR (ddPCR), polymerase spiral reaction (PSR), cross-priming amplification (CPA), enzyme-linked immunosorbent assay (ELISA), as well as peptide nucleic acid (PNA) based tools, aptamer-based tools, and lateral flow assays and different immune assay-based diagnostics for porcine diseases. As part of the Springer Protocols Handbooks series, the present book contains the type of in-depth knowledge on important diagnostics tools that provide a vital source of information for scientists, researchers, and students from different fields. Authoritative and practical, Protocols for the Diagnosis of Pig Viral Diseases aims to guide researchers toward developing new technologies for the diagnosis of pig pathogens.

This volume highlights natural products, molecular methods for identifying, and current trends in designing non-natural natural products. Chapters guide readers through protocols on heterologous expression techniques, gene disruption, modified pathway regulators, and in-vitro studies. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.Authoritative and cutting-edge, Engineering Natural Product Biosynthesis: Methods and Protocols aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge. Chapter 13 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

This second edition volume discusses the latest techniques and protocols used in the field that were not covered in the previous edition. The chapters in this book are organized into five parts. Part One looks at transcriptomic analyses and Part Two covers DNA replication and protein/DNA interactions. Part Three discusses translation dynamics, protein complexes, and proteomics. Part Four looks at genotypic screens and phenotypic profiling, and Part Five explores in silico integration of functional genomics data. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary material and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting edge and practical, Yeast Functional Genomics: Methods and Protocols, Second Edition is a valuable resource for all researchers interested in learning more about the evolving field of yeast.Chapters 1, 9, 16, 20, 22, 24, and 25 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

This second edition provides detailed practical information on important methods employed in the engineering of plant secondary metabolism pathways. New and updated chapters guide readers through extraction, quantification, purification, localization, characterization, data mining and processing, biosynthesis modulation, and pathway engineering of representative classes of plant specialized metabolites. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, list of necessary materials and reagents, tips on troubleshooting and known pitfalls, and step-by-step descriptions of readily reproducible protocols. Authoritative and cutting-edge, Plant Secondary Metabolism Engineering: Methods and Protocols, Second Edition aims to be a useful practical guide to help researchers working in this exciting field.

This third edition details new and updated methods and protocols on important databases and data mining tools. Chapters guides readers through archives of macromolecular sequences and three-dimensional structures, databases of protein-protein interactions, methods for prediction conformational disorder, mutant thermodynamic stability, aggregation, and drug response. Quality of structural data and their release, soft mechanics applications in biology, and protein flexibility are considered, too, together with pan-genome analyses, rational drug combination screening and Omics Deep Mining. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials, includes step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Data Mining Techniques for the Life Sciences, Third Edition aims to be a practical guide to researches to help further their study in this field.

This volume explores the fields of stem cell biology, regenerative medicine, and cancer biology. The chapters in this book cover topics such as embryonic stem cells (ES) and induced pluripotent cells (iPS); ES/iPSC derived tissue stem cells; tissue resident stem cells; assays for primitive malignant cells; and CRISPR/Cas9 engineering and transgenic model development. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Stem Cell Assays: Methods and Protocols is a valuable laboratory manual that allows researchers and scientists to learn about new techniques and approaches in this developing field.