Spektralanalyse, spektrokemi, massespektrometri

This fully updated volume describes methods and protocols for a number of drugs and toxins in a stepwise manner. Exploring the versatility and flexibility of mass spectrometry, the book covers the advantages of this technology, which typically include elimination of the need for special reagents such as antibodies, increased sensitivity and specificity, and multi-component analysis enabling the screening of tens to hundreds of compounds in a single assay run. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Clinical Applications of Mass Spectrometry in Drug Analysis: Methods and Protocols, Second Edition serves as a valuable resource for laboratory professionals who arealready utilizing mass spectrometry or considering bringing this technology to their labs.

Besides their notable historical and cultural significance, heritage buildings crucially contribute to the economy of those countries that rely heavily on the tourism industry. Investigation and monitoring of the origins of deterioration and damage are therefore key to the preservation of architectural heritage. Diagnosis of Heritage Buildings by Non-Destructive Techniques offers an up-to-date overview of state-of-the-art knowledge by collating specialized studies written by an international group of experts in the field, while also examining the value of these non-intrusive methods through a number of real-life case studies which prove NDT techniques’ global relevance. The volume is an invaluable reference resource for students, researchers, and practitioners alike.

This book includes basics of impedance spectroscopy, substrate compatibility issues, integration capabilities, and several applications in detection of different analytes. It helps explore the importance of this technique in biological detection, micro/nano-fabricated platforms and respective integration, and biological synthesis schemes.

This book describes analytical instruments widely used to characterize the nanostructured materials. It provides information about how to assess material quality, defects, the state of surfaces and interfaces, element distributions, strain, lattice distortion, and electro-optical properties of materials and devices. The information provided by this book can be used as a back-up for material processing, material design and debugging of device performance. The basic principles and methodology of each analysis technique is described in separate chapters, adding historic perspectives and recent developments. The data analysis, from simple to advanced level, is introduced by numerous examples, mostly taken from the authors' fields of research; semiconductor materials, metals and oxides. The book serves as a valuable guide for scientists and students working in materials science, physics, and engineering, who wish to become acquainted with the most important analytical techniques for nanomaterials.

This volume details aspects and applications of interfacing capillary electrophoresis (CE) with mass spectrometry (MS). Chapters guide readers through approaches based on different types of CE-MS interfaces such as (nano)sheath liquid, porous tip, and liquid junction, as well as various capillary coatings, and a broad range of applications including several top-down and bottom-up proteomic approaches. Additionally, a list of analyte targets was provided consisting of amphetamines, antibiotics, carbohydrates (including glycosaminoglycans and glycopeptides), enantiomers, extracellular matrix metabolites, monoclonal antibodies, and nanoparticles, and therefore covers numerous fields of applications such as pharmaceutical, biomedical, food, agrochemical, and environmental analysis. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Capillary Electrophoresis-Mass Spectrometry: Methods and Protocols aims to provide highly valuable information for both beginners and experts in the field be it students, technical staff, and scientists.

This textbook describes the theory underlying each instrumental procedure and applications of all instrumental methods. It comprehensively covers the instrumental methods of chemical analysis, chromatography, thermal methods of chemical analysis, electrochemical methods, and instrumental methods of analysis of inorganic compounds. These include thermogravimetric analysis, differential thermal analysis, thermometric titrations, and some miscellaneous thermal methods like derivative thermogravimetric analysis, thermobarography, differential scanning calorimetry, thermomechanical analysis, and electric thermal analysis, flame photometry, fluorimetry and phosphorimetry, nephelometric and turbidimetric techniques, refractory and interferometry, and X-ray methods. Each chapter consists a set of problems to aid self-learning. This textbook is highly useful for graduate and postgraduate students on chemistry and its allied fields. It can also be used as a quick reference material byprofessionals working in the various fields of chemistry and material science.

This book features selected papers presented at the 20th International Conference on Near Infrared Spectroscopy. It discusses the latest progress in the field of near infrared spectroscopy from around the globe, including the advances in instrumentation, spectral interpretation and Chemometrics. In addition, it presents potential trends for near infrared spectroscopy in the next decade and highlights developments in process analytical technology, chemical imaging and deep learning. It can be used as a reference book for researchers and application personnel engaged in spectroscopy technology, Chemometrics, analytical instruments, on-site rapid or on-line analysis, process control and other fields. It will also be useful for undergraduates and postgraduates studying these topics.

Guest-edited by Dr Michel Meyer (CNRS, Université de Bourgogne, Dijon) and Prof. Demetrio Milea (Università degli Studi di Messina), Emerging Analytical Techniques for Chemical Speciation Studies focuses on a selection of valuable instrumental methods for investigating complex formation equilibria in solution, providing information on the speciation (stoichiometry and in some instances structure of the formed species) but also allowing the measurement of thermodynamic parameters (equilibrium constants, reaction enthalpy and entropy). The title is split into two volumes belonging to the Series on Chemistry, Energy and the Environment. Part 1 (Volume 15 of this series), discusses various electrochemical, electromigration, and thermophoresis-based methods. Authored by eminent practitioners in their field, each of the four chapters covers both the theoretical and the practical aspects with helpful experimental guidelines. The latest technical and computational advances are described in a way that unambiguously show the major contributions of the authors at the top of their field. Hence, this book is intended to be a valuable introduction to newcomers, while being at the same time a helpful companion to more experienced users of each instrumental techniques. It provides an up-to-date overview with useful tips and hints on the application of selected cutting-edge analytical methods that allow unravelling and modelling intricate complex formation equilibria. So far, there has been no such book focussing specifically on the measurement of thermodynamic parameters, while covering such a wide panel of techniques. This book will be of interest to a broad readership, including analytical, coordination, supramolecular, environmental, instrumental, and physical chemists, radiochemists, electrochemists, and biochemists among others.

. The main aim of this book is to shine a spotlight on key experiments and their crucial importance for advancing our understanding of physics. Physics is an empirical science, and experiments have always been a driving force in the development of our understanding of nature. Facts matter. In that sense, the book attempts to be complementary to the many popularizations of theoretical physics, and to counterbalance the frequent emphasis there on more speculative ideas.Experimental physics is also an essential pillar in physics teaching, as well as helping broader audiences to better understand important concepts, particularly in challenging fields such as relativity or quantum physics, where our common sense intuition often fails.Readers are taken on an historical journey, starting with ¿Free Fall¿ and culminating in ¿Spooky Action at a Distance¿. En route they will encounter many important branches ofphysics, whose main ideas and theoretical description will be given a more empirical meaning. At the end, the reader is invited to reflect on what could be exciting and important directions for fundamental physics. All readers with an undergraduate degree in physical sciences or engineering will enjoy and learn much from this stimulating and original text.

Sample Handling and Trace Analysis of Pollutants: Innovations to Determine Organic Contaminants, Second Edition reviews the latest technologies and challenges in trace analysis of environmental pollutants, from selecting the right approach to tips for performing analytic procedures and measuring and reporting results. Written by internationally renowned experts in environmental analysis from 5 continents and edited by leaders in the field, this completely updated and revised volume presents the latest techniques developed over the past 10 years, such as high-resolution mass spectrometry, biosensors and imaging techniques. Important tools for problem-solving in the determination of environmental pollutants are also discussed.Chapters cover emerging pollutants in the environment, such as nanomaterials, microplastics, metabolites and/or transformation products and antimicrobial resistances. Specific sections describe field sampling techniques and sample preparation in environmental matrices: air, water, soil, sediment and biota, focus on passive samplers, cover the determination of these environmental contaminants based on analytical techniques, such as the use of gas chromatography and liquid chromatography coupled to mass spectrometry, immunoassays, and biosensors as well as advanced analytical methods such as imaging techniques.

This book offers historical and state-of-the-art molecular spectroscopy methods and applications in dynamic compression science, aimed at the upcoming generation in physical sciences involved in studies of materials at extremes. It begins with addressing the motivation for probing shock compressed molecular materials with spectroscopy and then reviews historical developments and the basics of the various spectroscopic methods that have been utilized. Introductory chapters are devoted to fundamentals of molecular spectroscopy, overviews of dynamic compression technologies, and diagnostics used to quantify the shock compression state during spectroscopy experiments. Subsequent chapters describe all the molecular spectroscopic methods used in shock compression research to date, including theory, experimental details for application to shocked materials, and difficulties that can be encountered. Each of these chapters also includes a section comparing static compression results. The last chapter offers an outlook for the future, which leads the next-generation readers to tackling persistent problems.

This volume details protocols on mass spectrometry and associated techniques. Chapters guide readers through micro- and macronutrients analysis, mass spectrometry-related methodologies, direct insertion, matrix-assisted laser desorption ionization (MALDI), gas chromatography (uni- and bi-dimensional), liquid chromatography, plasma mass spectrometry (ICP-MS), and analyses in food samples.  Authoritative and cutting-edge, Mass Spectrometry for Food Analysis aims to provide comprehensive and updated state-of-art methodologies and models for food analysis.

This book describes a simple yet innovative method for performing Raman spectroscopy of samples submerged under liquid nitrogen. While Raman spectroscopy has proven to be a powerful tool for the characterization of the structure of matter in the gaseous, liquid, and solid phases, one major difficulty in its application has been laser damage to the material under investigation, especially for biological samples. This book demonstrates how immersion of the sample in liquid nitrogen protects the sample from thermal degradation and oxidation at high incident laser power and allows improvements in sensitivity and spectral resolution over room-temperature Raman spectroscopy, leading to the so-called RUN (Raman Spectroscopy Under liquid Nitrogen) technique. Cooling to liquid nitrogen temperature also allows the selection of the lowest energy molecular conformation for molecules which may have many low energy conformers. In addition, the presence of liquid nitrogen over a roughened surface improves the sensitivity of Surface Enhanced Raman Spectroscopy (SERS), enabling the closely related SERSUN (Surface-Enhanced Raman Spectroscopy Under liquid Nitrogen) technique. This book starts with the theoretical and experimental basics of Raman and polarized Raman spectroscopy, before moving on to detailed descriptions of RUN and SERSUN. Room temperature and RUN spectra are provided for over fifty molecules.

The nonlinear optical spectrum signal technology is a new type of optical characterization technology owing to its non-invasiveness and good biocompatibility. This book highlights a comprehensive introduction to the Stimulated Raman scattering (SRS), Anti-Stokes Raman Spectroscopy (CARS), Two-photon Excited Fluorescence (TPEF) and Second Harmonic Generation signals (SHG) technologies. The four types of nonlinear optical signals technologies, especially two-dimensional and three-dimensional imaging, have great application potential in physics, materials science, chemistry and biomedicine. The book covers principles, theoretical calculation methods, signal measurement methods and imaging specific methods. The theoretical part starts from the basics of nonlinear optics and the relationship with strong light, and gradually transitions to theoretical calculation methods for specific optical signals. it combines the classical theory and the quantum theory to help readers develop a thorough understanding of the technologies. The book is a good reference for graduate students majored in physics and chemistry and for researchers working on optics, photonics and materials science.

This book gives a comprehensive account of modern x-ray science, based on the use of synchrotron radiation and x-ray-free electron lasers (XFELs). It emphasizes the new capabilities of XFELs which extend the study of matter to the intrinsic timescales associated with the motion of atoms and chemical transformations and give birth to the new field of non-linear x-ray science. Starting with the historical understanding of the puzzling nature of light, it covers the modern description of the creation, properties, and detection of x-rays within quantum optics. It then presents the formulation of the interactions of x-rays with atomic matter, both, from semi-classical and first-principles quantum points of view. The fundamental x-ray processes and techniques, absorption, emission, Thomson, and resonant scattering (REXS and RIXS) are reviewed with emphasis on simple intuitive pictures that are illustrated by experimental results. Concepts of x-ray imaging and diffractive imaging of atomic and nano structures are discussed, and the quantum optics formulation of diffraction is presented that reveals the remarkable quantum substructure of light. The unique power of x-rays in providing atom and chemical-bond specific information and separating charge and spin phenomena through x-ray polarization (dichroism) effects are highlighted. The book concludes with the discussion of many-photon or non-linear x-ray phenomena encountered with XFELs, such as stimulated emission and x-ray transparency.

This two-volume book provides an enriching insight into the laser, covering different types of lasers, the basic science behind the technology, their role at the cutting-edge of current scientific research, and their wide-ranging applications. With just high school physics as a prerequisite and favoring qualitative yet scientifically sound explanations over high-level mathematics, this book is aimed at a broad spectrum of readers in physics, chemistry, engineering, medicine, and biology. Its engaging and lucid presentation is enhanced with plenty of illustrations, making the world of the laser accessible to undergraduate students in the sciences and any other inquisitive readers with high school physics under their belts. Furthermore, the text is often laced with anecdotes, picked from history, that are bound to pique the minds of the readers. It is ideal for self-study or as a complement to courses on optics and optoelectronics. This volume, Part 1 of 2, explains the fundamentals of optics, what a laser is, how it works, and what is unique about the light it emits, from fundamental quantum theory through population inversion and cavity to common laser types. It is followed by Part 2 which depicts the many advances in science enabled by the laser, including spectroscopy, nonlinear optics, optical cooling and trapping, and optical tweezers, among many others, and provides a glimpse into the ways that the laser affects our lives via its uses in medicine, manufacturing, the nuclear industry, energy, defence, communication, ranging, pollution monitoring, art conservation, fashion, beauty, and entertainment.

This book discusses chemometric methods for spectroscopy analysis including NIR, MIR, Raman, NMR, and LIBS, from the perspective of practical applied spectroscopy. It covers all aspects of chemometrics associated with analytical spectroscopy, including representative sample selection algorithm, outlier detection algorithm, model updating and maintenance algorithm and strategy and calibration performance evaluation methods.To provide a systematic and comprehensive overview the latest progress of chemometric methods including recent scientific research and practical applications are presented. In addition the book also highlights the improvement of classical algorithms and the extension of common strategies. It is therefore useful as a reference book for researchers engaged in analytical spectroscopy technology, chemometrics, analytical instruments and other related fields.

This book summarizes the recent developments of mass spectrometry techniques in microbial analysis for researchers in the field and for those who are about to enter the field.

This book provides the reader with an updated comprehensive view of the rapidly developing and fascinating field of fluorescence spectroscopy and microscopy. In recent years, fluorescence spectroscopy and microscopy have experienced rapid technological development, which has enabled the detection and monitoring of single molecules with high spatial and temporal resolution. Thanks to these developments, fluorescence has become an even more popular method in physical, biological and related fields. This book guides the reader through both basic and advanced fluorescence spectroscopy and microscopy approaches with a focus on their applications in membrane and protein biophysics. Each of the four parts: A - Fluorescence Spectroscopy, B - Fluorescence Microscopy, C - Applications of Fluorescence Spectroscopy and Microscopy to biological membranes and D - Applications of Fluorescence Spectroscopy to protein studies are written by experts within the field. The book isintended for both complete beginners who want to quickly orient themselves in the large number of existing fluorescent methods, as well as for advanced readers who are interested in particular methods and their proper use.Chapter ¿Dynamics and Hydration of Proteins Viewed by Fluorescence Methods: Investigations for Protein Engineering and Synthetic Biology¿ is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Infrared Spectroscopy - Perspectives and Applications is a compendium of contributions from experts in the field of infrared (IR) spectroscopy. This assembly of investigations and reviews provides a comprehensive overview of the fundamentals as well as the groundbreaking applications in the field. Chapters discuss IR spectroscopy applications in the food and biomedicine sectors and for measuring transport through polymer membranes, characterizing lignocellulosic biomasses, detecting adulterants, and characterizing enamel surface advancements. This book is an invaluable resource and reference for students, researchers, and other interested readers.

This second edition details new and updated chapters on key methodologies and breakthroughs in the mass spectrometry imaging (MSI) field. Chapters guide readers through nano-Desorption Electrospray Ionisation (nDESI), Matrix Assisted Laser Desorption Ionisation-2 (MALDI-2), Laser Ablation - Inductively Coupled Plasma-Mass Spectrometry (LA-ICP-MS) ,Imaging Mass Cytometry (IMC) with a variety of diverse samples including eye tissue, crop analysis, 3D cell culture models, and counterfeit goods analysis. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Imaging Mass Spectrometry: Methods and Protocols, Second Edition aims to be a useful and practical guide to new researchersand experts looking to expand their knowledge.

This book focuses on advanced optical spectroscopy techniques for the characterization of cutting-edge semiconductor materials. It covers a wide range of techniques such as Raman, infrared, photoluminescence, and cathodoluminescence (CL) spectroscopy, including an introduction to their physical fundamentals and best operating principles. Aimed at professionals working in the research and development of semiconductors and semiconductor materials, this book looks at a broad class of materials such as silicon and silicon dioxide, nano-diamond thin films, quantum dots, and gallium oxide. In addition to the spectroscopic techniques covered, this book features a chapter devoted to the use of a scanning electron transmission microscope as an excitation source for CL spectroscopy. Written by a practicing industry expert in the field, this book is an ideal source of reference and best-practices guide for physicists, as well as materials scientists and engineers involved in the area of spectroscopy of semiconductor materials. Further, this book introduces the cutting-edge spectroscopy such as optical photothermal IR and Raman spectroscopy or terahertz time-domain spectroscopy (THz-TDS) etc.

This monograph is a fundamental reference for scientists and engineers who encounter spin processes in their work. The author, Ilya Kuprov, derives the concept of spin from basic symmetries and gives an overview of theoretical and computational aspects of spin dynamics: from Dirac equation and spin Hamiltonian, through coherent evolution and relaxation theories, to quantum optimal control, and all the way to practical implementation advice for parallel computers.

Nanospectroscopy addresses the spectroscopy of very small objects down to single molecules or atoms, or high-resolution spectroscopy performed on regions much smaller than the wavelength of light, revealing their local optical, electronic and chemical properties. This work highlights modern examples where optical nanospectroscopy is exploited in photonics, optical sensing, medicine, or state-of-the-art applications in material, chemical and biological sciences. Examples include the use of nanospectroscopy in such varied fields as quantum emitters, dyes and two-dimensional materials, on solar cells, radiation imaging detectors, biosensors and sensors for explosives, in biomolecular and cancer detection, food science, and cultural heritage studies. Also by the editors: Textbook "Optical Nanospectroscopy": _"Fundamentals & Methods" (Vol. 1) and _"Instrumentation, Simulation & Materials" (Vol. 2).