Bioteknologi

This third edition provides new and updated chapters detailing preparation of liposomes, physicochemical characterization of liposomes, lipid analysis, drug encapsulation, surface modification, stimuli response as well as cellular interaction, and biodistribution. Also included is an updated chapter on the history and evolution of the field of liposomology. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Liposomes: Methods and Protocols, Third Edition aims to serve as a reference for graduate students, post-doctoral researchers as well as established investigators utilizing lipid-based systems.

This volume provides comprehensive dry and wet experiments, methods, and applications on nanopore sequencing. Chapters guide readers through bioinformatic procedures, genome sequencing, analysis of repetitive regions, structural variations, rapid and on-site microbial identification, epidemiology, and transcriptome analysis. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and methods, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Nanopore Sequencing: Methods and Protocols aims to be comprehensive guide for researchers.

Nanophototherapy: Preparations and Applications provides a comprehensive overview of the various multifunctional nanoparticles used for phototherapy, with an emphasis on fundamental nanotechnology and the latest research of photothermal therapy (PTT) and photodynamic therapy (PDT). The different types of phototherapeutic nanomaterials are thoroughly described, along with their structural features and synthesis. This is the first book to cover nanomaterial-based phototherapy for both cancer and bacterial infections. It is an essential resource for researchers, academics, and professionals interested in the potential of multifunctional nanomaterials for therapeutic applications.

Advances in Sustainable Applications of Microalgae discusses different mechanisms used by microalgae to treat various gaseous streams, wastewaters, and pollution bioremediation. In addition, the advantages of the application of microalgal biomass in the agricultural and food/feed sectors are presented and bioenergy from microalgae with a view to sustainability is explored. The present covers the use of microalgal cultures in various applications, such as: the treatment of gaseous streams by removing various contaminants, wastewater treatment through the removal of several pollutants, biomass valorization for agriculture purposes, bioenergy from microalgae, and industrial integration of all these processes in a biorefinery concept. Finally, the book emphasizes the importance of gathering scientific knowledge to overcome drawbacks related to microalgal production and develops the concept of biorefinery based on microalgal biomass for a more sustainable future.

Smart Biomimetic Coatings: Design, Properties and Biomedical Applications summarizes vital research in multifunctional (i.e., anti-corrosion, anti-fouling, and self-cleaning properties) surface coatings in various applications such as orthopedic and dental implants, tissue engineering, sensors and more. The book introduces biomimetic coatings, aiding understanding of highly complicated architectures in natural composites, along with discussions on the importance of proper mimicking to achieve composite materials with exceptional performance. The synthesis and unique properties of smart biomimetic coatings are detailed before a wide selection of biomedical applications is explored. This is an important reference of interest to researchers and R&D groups working in materials science, biomedical engineering, tissue engineering, and implant design.

This book focuses on the various applications of microorganisms for sustainable environment and the reduction of hazardous pollutants released in various forms, including xenobiotics, e-waste, pesticides, insecticides, plastic, heavy metals, paper waste, medical waste, textile dyes, and their impact on environmental and human health. The book involves a series of research reports that explain the application of microbes used to solve real-life issues raised due to changes happening in environment, including pollution, by covering applications, including the use of bacteria, fungi, microalgae and biofilm in the detection and degradation of crude oil, pesticides, dyes, e-waste, heavy metals and other pollutants. It also focuses on integrative strategies in the application of microbial nanomaterials for remediation of pollutant. This material will help environmental scientists and microbiologists to learn about existing environmental problems and suggest novel ways to control or contain their effects by employing various treatment approaches.

Medical Nanobiotechnology: Nanomedicine for Repair, Regeneration, Remodelling, and Recovery thoroughly reviews the potential of functionalized biomaterials as ideal candidates for nanomedicine. The book covers advances in the development of nanotheranostic agents that can simultaneously help in both effective therapy and rapid diagnosis. A range of materials is covered, including their fabrication, characterization and assessment, as well as their functionalization and incorporation into implants and medical devices. Clinical aspects and challenges are discussed, helping bridge the gap between laboratory research and the translational impact as nanomedicine begins to develop point-of-care customized therapy. This book is an interdisciplinary reference for researchers and R&D groups interested in the development of novel nanobiomaterials for therapeutic applications.

Endophytes have unique characteristics to propagate inside host plants and aide the host by providing additional defense against environmental stresses and phytopathogens. Among endophytes, endophytic fungi have been recognized as a proliferating source for the production of bioactive compounds with potential in both the agricultural and health industries.Excessive use of pesticides in agriculture as well as in the food supply chain have led to increased antimicrobial resistance (AMR), which is one of the more serious global health concerns. Endophytic fungi and their associated bioactive substances have been reported to show natural ability to fight against pathogens and can reduce the development of AMR in nature. Studies also reported their potential as producers of hormones that enhance plant growth and several compounds having biological activities without any adverse effects.This book covers the pharmaceutical potential offungal endophytes as elucidated through advanced bioinformatics tools and modern techniques. Also synthetic drugs are losing their efficacy to treat microbial-induced disease due to this emergence of drug-resistant microbes. Therefore, insight into the detection of valuable plant constituents is a pressing priority in order to achieve effective pharmaceutical treatments. This book provides lucid discussion of the most recent research and provides knowledge about the applications of endophytic fungi with a specific focus to their pharmaceutical potential.

The world population is increasing while arable land is decreasing at an alarming rate. About one-quarter of arable land is degraded and needs significant restoration before it can sustain crops again. By 2030, the water supply will fall 40% short of meeting global demand. Moreover, looming climate change poses additional challenge to increasing food production to feed 10 billion people by 2050. Current major agricultural systems are on a largely unsustainable trajectory because of their contributions to greenhouse gas emissions, water pollution, and biodiversity loss.For these reasons, innovative technologies are being introduced in modern agriculture to sustain food production. They include digital and geospatial technologies to manage soil, climate and plant genetic resources. With the development of tools and sensors integrated into the internet of things (IoT) environment, physically collected information is converted into computer-readable language. Digital innovations thus allow real-time analysis, machine learning, and artificial intelligence (AI) that manage massive amount of data, also known as big data. Accordingly, digital agriculture affords greater potential for sustainable farming and economic benefits.This book summarizes the latest advances in AI-integration of agriculture practices. Specific focus includes but not limited to, big data, yield mapping, pests management, and optimal fertigation. As such, it presents a forward-looking approach to meet multiple UN Sustainable Development Goals, specifically, SDG 2, 6, 13 and 15.

¿¿¿¿¿¿¿¿¿¿¿ ¿¿¿¿¿¿¿¿¿: ¿¿¿¿¿¿ ¿¿¿¿¿¿¿¿¿¿¿¿ ¿¿¿¿¿¿ ¿¿¿¿¿¿ ¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿ ¿¿¿¿¿¿¿¿¿ ¿¿¿¿¿¿ ¿¿¿¿¿¿¿¿, ¿¿¿¿¿¿ ¿¿¿¿¿¿¿¿¿¿ ¿¿¿¿¿¿¿¿¿¿ ¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿ ¿¿¿¿¿¿¿¿¿¿¿¿¿ ¿¿¿¿¿¿¿¿¿¿¿¿. ¿¿ ¿¿¿¿¿¿, ¿¿¿¿¿¿¿¿¿¿¿¿¿, ¿¿¿¿¿¿¿ ¿¿¿¿¿¿¿¿¿¿ ¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿ ¿¿¿¿¿¿¿¿¿ ¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿. ¿¿¿¿¿¿¿¿¿¿¿ ¿¿¿¿¿¿¿¿¿ ¿¿¿¿¿¿ ¿¿¿¿¿¿¿¿¿¿¿¿¿¿ ¿¿¿¿¿ ¿¿¿¿¿¿¿¿¿¿ ¿¿¿¿¿¿¿¿¿¿¿¿¿¿, ¿¿¿¿ ¿¿¿¿¿¿¿¿¿, ¿¿¿¿¿¿¿, ¿¿¿¿¿¿¿¿¿¿, ¿¿¿¿¿¿¿ ¿¿¿¿¿¿ ¿¿¿¿¿¿¿¿¿¿¿¿.Biomedical devices: Their crucial role in healthcare Biomedical devices are instruments used to address medical and healthcare-related issues. They are designed on the foundations of biology, engineering, and medicine. Biomedical devices are used in all areas of healthcare, including diagnosis, treatment, rehabilitation, and care. Role in diagnosis Biomedical devices play a vital role in diagnosis. They are used to detect diseases and conditions, assess the severity of an illness, and monitor patient progress. Some examples of biomedical devices used in diagnosis include: Medical imaging devices such as X-ray machines, MRI machines, and CT scanners. These devices use radiation or sound waves to create images of the body's internal structures.Laboratory testing devices such as blood analyzers and urinalysis machines. These devices are used to measure the levels of chemicals and other substances in the blood or urine.Monitoring devices such as heart monitors and pulse oximeters. These devices are used to track a patient's vital signs.

Therapeutic macromolecules and biologics have gained increasing importance in the last decade. Pulmonary and intranasal delivery of these therapeutic modalities, which have poor oral bioavailability, could constitute an attractive, non-invasive alternative to parenteral delivery. They can be considered for either topical use for treating diseases affecting the airways or for systemic use for treating a variety of other diseases. Therefore, this book is a readable, thoughtful, and useful guide on the latest research developments and the best options that people involved with pulmonary and intranasal route of administration and biologically active agents can bring to their practices. Furthermore, it explores the underlying scientific rationale for the strategies applied to overcome drug delivery challenges and provides insights to inspire further research in this growing area.

This book introduces biomass which is utilized all over the world based on geographical, cultural, and historical background. It covers 18 major biomass types and several specific plants categorized into 3 groups based on their usage. The present and historical background of representative materials from biomass, such as cellulose, lignin, chitin, sugar, molasses, amylose, and other interesting natural biopolymers, such as hyaluronan, gum Arabic, and others are introduced. Furthermore, characteristic features of representative and influential plants, such as rice, eucalyptus, and oil palm are described together with historical episodes. Although physicochemical characteristic properties of each material and plant have been published over many decades, scarcely a comprehensive introduction on biomass together with Asian, European, and Latin American cultural backgrounds. In this book, biomass familiar to everybody¿s life is introduced based on scientific and cultural viewpoints. It guides readers to gain background knowledge of targeting biomass to be developed as industrial resources. In addition to students, scientists, and lecturers, the book will be useful for industrial engineers, both specialists in polymer science and technology and materials experts.

This book aims at bringing out comprehensive information on cropping systems modelling in the world. The major focus of this book is to address the integration of soil, plant and environmental interactions for climate smart agriculture. This book covers aspects of application of Decision Support System for Agrotechnology Transfer (DSSAT) for climate smart agriculture. Step-by-step details of application of modelling approaches used for various cropping systems under changing climate are provided which are being adopted by farmers in the world. The book is enriched with figures or diagrams to show the various mechanisms involved to support the decision making for climate change adaptations. Essential information is given regarding crop models calibration, evaluation and application and every individual chapter is comprised of a specific cropping system. Further contents include integration of climate models and crop models for refining the decisions for sustaining the production of various cropping systems for climate smart agriculture. This book assists the agricultural scientists involved in research regarding climate smart agriculture for improving the standards of agricultural research for ensuring food security under changing climate. This is also equally useful for policy makers being involved in future planning.

This edited book is a comprehensive compilation of applications of plant molecular farming in various fields including agriculture, industry and medicine, using different plant/crop systems as bioreactors for the bulk production of products of interest. The book places special emphasis on plant molecular farming being utilized for the bulk production of biologics, industrial enzymes, vaccines, drugs and recombinant pharmaceutical proteins.Plant molecular farming (PMF) is an upcoming promising interdisciplinary subject, and therefore the book contains contributions from experts across the disciplines. The utilization of plant viral vectors to produce biologics, virus-like particles and antimicrobial peptides via PMF have been highlighted. Molecular farming for the bulk production of recombinant pharmaceutical proteins and various types of human and veterinary vaccines in different crops/plants and genetically modified seeds has been included. The process of scaling up to manufacturing capacity, product formation by bioprocessing of plant cell suspension culture, and bioreactor engineering have been incorporated. Lastly, the book provides special coverage on utilizing PMF made products to control neglected tropical diseases and plant-made antigens as immunotherapies for allergic and autoimmune diseases.The book has chapters from leading experts on the subject, making it equally beneficial for researchers, policy planners, industrialists, medical professionals, entrepreneurs, and students of various related disciplines.

This book elucidates the sustainable production of commercially important biomolecules in medicines, food, and beverage processing, through biological systems, including microorganisms, animal cells, plant cells, tissues, enzymes, and in vitro. It discusses promising technologies for the manipulation of cells including, genetic engineering, synthetic biology, genome editing, and metabolic engineering. The initial chapters of the book introduce topics on biomanufacturing, circular economy, strain design and improvement, upstream and downstream processing. The subsequent chapters cover artificial intelligence-assisted production, designer cell factories, biosensors for monitoring biomolecules, different cells factories, biosynthetic pathways, and genome editing approaches for scale-up biomanufacturing. Lastly, the book discusses the opportunities and challenges of implementing biological systems for the production of biomolecules.¿This book is a valuable source for students, researchers, scientists, clinicians, stakeholders, policymakers, and practitioners to understand biomanufacturing for the sustainable production of biomolecules.

This detailed volume presents a series of protocols dealing with different aspects of inclusion body (IB) processing, from cloning procedures to purification of refolded product. Commencing with chapters on upstream processing, looking into different expression strategies for IB production, the book continues with downstream applications, highlighting early protein purification and subsequent analytics, as well as success stories of IB-based processes. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Inclusion Bodies: Methods and Protocols serves as an ideal resource for facilitating diverse aspects of IB processing.

This volume explores the latest developments in the study and application of phage biology. The chapters in this book are divided into five parts and cover topics such as phage display, selection, and evolution; genetic and chemical modification of phages; analyzing structures by electron microscopy; characterizing phage transcripts and proteins; and the biology of whole phages. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and comprehensive, Phage Engineering and Analysis: Methods and Protocols is a valuable resource that will help both expert and novice researchers - from backgrounds ranging from microbiologists to biophysicists to chemical engineers - further enhance their understandingof this important and evolving field.

Leveraging Metaverse and Analytics of Things (AoT) in Medical Systems explores the potential benefits and applications of emerging technologies such as metaverse and AoT in the field of healthcare. The book provides insights into how these technologies can be leveraged to improve the efficiency, effectiveness, and quality of medical systems. It explores the concept of metaverse and its potential applications in healthcare, including the use of virtual and augmented reality technologies for medical education, training, and simulation, as well as the development of immersive environments for patient care and therapy. The book also delves into the field of AoT, such as the use of wearable devices, smart sensors, and other connected technologies. Applications to monitor patient health, track medical outcomes, and inform clinical decision-making are covered. Integrating both technologies can help improve medical training, diagnosis, treatment, and patient outcomes through the use of virtual reality and real-time data analytics.

Dive into the intricate realm of osteoporosis through the lens of biomechanics with Biomechanical Insights into Osteoporosis. This pioneering volume offers a comprehensive exploration of this debilitating condition, providing an in-depth understanding of its biomechanical foundations and implications. Osteoporosis, characterized by reduced bone mineral density and heightened susceptibility to fractures, stands as a global health challenge. This book brings together leading experts in the field, who rigorously analyze the multifaceted aspects of osteoporosis, offering invaluable knowledge for researchers, clinicians, and students alike.Key Highlights:¿Biomechanical Foundations: Gain profound insights into the biomechanical basis of bone fractures and fracture osteosynthesis in small animals. Explore the fundamental principles that shape our understanding of osteoporotic biomechanics. ¿Non-Glucocorticoid Drug-Induced Osteoporosis: Investigate the mechanisms, risk factors, and therapeutic approaches for osteoporosis induced by medications other than glucocorticoids. ¿Vascular Remodeling and Bone Destruction: Discover the common pathogenetic links between vascular remodeling and bone tissue destruction in postmenopausal women with arterial hypertension. This holistic perspective illuminates bone health dynamics. ¿Genetic Targets and Future Directions: Explore promising genetic targets that have the potential to revolutionize osteoporosis treatment. Get a glimpse into the future of research and treatment strategies. ¿Biomarkers and Metabolism: Delve into the latest research progress surrounding bone mineral density and bone metabolism biomarkers, offering valuable insights into diagnostics and monitoring. ¿Impact of Diseases and Medical Treatments: Understand the intricate interplay between diseases and medical treatments on bone mineral density. Gain a comprehensive understanding of how various factors influence skeletal health.This book represents a significant milestone in deciphering the complexities of osteoporosis from a biomechanical perspective. Join us in this scientific journey to explore the fascinating facets of osteoporosis. Biomechanical Insights into Osteoporosis is an essential resource for anyone seeking a deeper understanding of this critical health issue and the biomechanical aspects that underlie it.

This volume discusses: (1) the treatment of hazardous sludge, wastewater, textile effluent, contaminated groundwater, laboratory waste, toxic dye, heavy metals, acid mine drainage and palm oil effluent; (2) the technologies of stabilization, solidification, natural coagulation-flocculation, river catchment control and mitigation, dredging and mining operations, and (3) the management of acid mines, laboratories, nano pollutants and plant effluents.

This volume ¿Cell Engineerring 11 - Biopharmaceutical Manufacturing: Progress, Trends and Challenges¿ is a source of the latest innovative research and technical development in biomanufacturing systems. It is organised into 2 parts: 1) Manufacturing of recombinant therapeutic proteins (e.g. therapeutic antibodies, biosimilars/biogenerics) and 2) Manufacturing aspects of cell and gene therapy. Each with selected chapters on the following topics for both up- and downstream, such as: Advanced process strategies, especially continuous manufacturing, Advanced culture techniques, especially single-use systems, Process transfer, scale-up/scale-down models, Processing advances/Manufacturing productivity/efficiency, Model-assisted process understanding and development/Digital Twins, Process controls and analytics, Quality control, Quality by design, Facility design and full-scale commercial systems, manufacturing technology innovation.The book comprises contributions of experts from academia and industry active in the field of cell culture development for the production of recombinant proteins, cell therapy and gene therapy, with consideration of Digital Twin¿s and facility design. The knowledge and expertise of the authors cover disciplines like cell biology, engineering, biotechnology and biomedical sciences. Inevitably, some omissions will occur in the test, but the authors have sought to avoid duplications by extensive cross-referencing to chapters in other volumes of this series and elsewhere. We hope the volume provides a useful compendium of techniques for scientists in industrial and research laboratories active in this field.

Lanthanides in Enzymology and Microbiology, a new volume in the Foundations and Frontiers in Enzymology Series, offers a detailed discussion of lanthanides and lanthanide-dependent enzyme biology. In this book, more than a dozen global experts consider lanthanide enzymology fundamentals, organismal utilization of lanthanides, distribution and diversity of lanthanide-dependent enzymes, regulation of intracellular levels of lanthanides, gene expression regulation via lanthanides, as well as likely applications of lanthanide binding proteins. Lanthanide-dependent methanol and alcohol dehydrogenase metabolism are considered in both methylotrophs and non-methylotrophs, alongside various application areas, from recovery of rare earth elements to developing lanthanide ion binding peptides and biosynthesis of terpolymers through reverse-oxidation pathways. In providing this deep context and pathways for future research, this book acts as an invaluable resource in this emerging field for researchers and students of biochemistry, biotechnology, and environmental microbiology alike.