Cross-species amplification of micro-satellites in Coriander - Sharda Choudhary - Bog

Coriander is a spice and vegetable crop having prime position in flavouring food. Very limited literature is available for molecular studies in coriander it¿s a need to develop molecular markers for this crop. Development of SSRs (Simple Sequence Repeats) for new crop like coriander is an expensive and time consuming process, but this can be easily achieved by transferring the microsatellile loci from the same family/genus/species which is a cost-effective approach for development of SSRs in lesser studied crops like corianderdeveloping microsatellite markers for new species. Presently carrot and celery SSRs are available on the public domain for cross-species investigation, both crops are from the same family, Apiaceae. We examined the transferability of hundred carrot (Daucuscarota) SSR loci to coriander (Coriandrum sativum L.). Thirty nine percent primer (39/100) amplified SSRs for coriander. Out of 39 SSR loci, 35 primers were monomorphic and specific to carrot primers, showing approximately the same size of SSR loci as reported for carrot. Four SSRs were polymorphic, seven primer pairs were non-specific which were deviating for band size from the source of SSRs.