Immunologi

A research-backed guide to healing your gut and boosting immunity

This book discusses principles, methodology, and applications of microbiological laboratory techniques . It lays special emphasis on the use of various automated machines that are essential for medical microbiology and diagnostic labs. The book contains eleven major chapters. The first chapter describes the good lab practices which should be followed by the students in all biological, chemistry or microbiology laboratories. The next chapter describes manual and automated characterization of antibiotic resistant microbes, followed by a chapter on genomics based tools and techniques that are integral to research. Further chapters deal with other important techniques like immunology based techniques, spectrophotometry and its various types, MALDI-TOFF and microarrays, each with illustrations and detailed description of the protocols and applications. The book also gives certain important guidelines to the students about the planning the experiment and interpreting results.The book is highly informative and provides latest techniques. It is a handy compendium for graduate and post graduate students, as well as more advanced researchers.

This volume provides an updated collection of protocols for manipulating and studying VEGF signaling pathways in vitro and in vivo and aims to present a range of both firmly established and newly emerging technologies. Covering multiple model species, from mouse to zebrafish to human, the book explores the role of VEGF and VEGFR isoforms in exosomes, cultured cells, or in tissues, as well as robust cell assays for the investigation of basic angiogenic mechanisms and VEGF signaling in more complex cellular systems, amongst other subjects. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, VEGF Signaling: Methods and Protocols, Second Edition provides a useful tool for researchers in the vascular biology community and beyond in understanding the basic biology of VEGF signaling and in translating this research into the clinic.

The plethora of miscommunication and disinformation about how SARS-CoV-2 (COVID-19) spreads suggests a widespread misunderstanding of how viruses work. This book will focus on the interpretation of scientific and medical results, giving the reader guidance on interpreting virological data, including the concepts of 'live' versus infectious virus.

This book addresses the stabilization of vaccine powders by spray drying and provides an overview of the current state of the art on a laboratory and industrial scale. The book aims to familiarize readers with the advances in vaccine spray drying technology to understand its application potential better. In particular, the book addresses the design of aseptic spray dryers, parameters affecting the spray drying process, sterile powder processing, cleaning procedures, and powder filling. In addition, different drying technologies for the production of dry powder vaccines are compared to discuss the unique capabilities of spray drying as a particle technology for vaccines. Special attention is given to research studies on spray-dried vaccines published over the past 30 years, with key findings from laboratory research to clinical trials. Potential applications of spray-dried vaccines and routes of administration are presented in detail. Finally, an outlook is given on how close the aseptic spray-drying of vaccines is to the market and the challenges that need to be overcome to be commercially successful. The book's target audience is academics, researchers, vaccine developers, industry experts, students, and possibly funders, including government agencies, who are active in the field. In addition, the book is a reference source for those involved in the vaccine formulation and biopharmaceutical processing industry.

This book is about recurring celiac disease, its diagnosis and management. It starts by discussing the pathogenesis of refractory celiac disease and the role of viruses, microbiota and genetics in this disease. It also covers the epidemiological aspects.Refractory Celiac Disease is a unique resource on the topic written by a team of international experts in the topic and will be of great use to gastroenterologists and researchers in the field.

Imaging Inflammation provides updates on cutting-edge imaging methods being applied to problems in inflammation research. From state-of-the-art research tools to diagnostic tests, and from single-cell to whole-body imaging, this volume offers a comprehensive overview of how imaging experts across a range of disciplines are expanding our understanding of inflammation and immunity.

This edited collection of 12 chapters by research workers from a wide range of disciplines resolves the confusion that currently surrounds the "e;hygiene hypothesis"e; by considering the human need for exposure to microorganisms from an evolutionary point of view.  The book explains why we evolved a requirement for exposure to microbiota from our mothers, from other people, and from the natural environment. It also explains the physiological roles of these exposures, what goes wrong when the exposures are distorted and how human lifestyles and activities, including degradation of the natural environment, are leading to this distortion.  Particular attention is given to the range of pathologies associated with inappropriate microbial exposures and inappropriate colonization, including immunoregulatory problems such as allergies and autoimmunity, metabolic problems such as obesity and diabetes, and problems of central nervous system function and neurodegeneration.  This book is of profound relevance to most medical disciplines, but also to those concerned with preserving the natural environment and with developing healthier urbanisation.

An essential guide to optimising your immunity and feeling your best - all year roundThousands of us are struck down by colds, flu and other viruses every year. We know we should be taking steps to look after our immune system every day, but beyond taking vitamin C and getting a good night's sleep it's often difficult to know where to start. In How to Stay Healthy, registered nutritionist Jenna Hope provides an easy-to-follow guide that will show you the steps you need to take to optimise your immunity and feel at your best. Through well-explained science this book will help you to understand what the immune system is, why it is so important and how to keep it functioning well in a way that suits you. It will cover the impact of nutrition, gut health and the role of supplements on your immune system, as well as lifestyle factors including smoking, physical activity, sleep, stress and pollution. Hope's smart, simple and sustainable approach will show you easy ways to incorporate immunity friendly foods into your diet, quick tips for when you're feeling run down and guidance to set you on the road to recovery from illness. Looking after your immune system has never been more important. This book provides the expert advice you need to do just that.

This detailed book provides a broad collection of methodologies for natural killer (NK) cell research. Beginning with an assortment of methods for the isolation of NK cells and NK cell differentiation, the volume continues with methodologies for functional tests, such as cytotoxicity, viral infection, and metabolism assays, as well as clinical applications of NK cells. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Natural Killer (NK) Cells: Methods and Protocols serves as an ideal guide for researchers looking to contribute to the further development of basic and clinical NK cell research.

This book intends to investigate the broad spectrum of genetic changes in immunological processes involved in cutaneous diseases. One of the main goals of immunogenetic studies is finding susceptibility genes for complex diseases. This can provide an insight into the pathogenesis of the condition in a way that is not easily achievable through other kinds of studies. Thus they are a rational initial step for generating hypotheses about disease pathogenesis. This may especially benefit dermatology, a field notorious for having too many diseases with unknown etiologies.Immunogenetic investigations have made targeted treatment strategies possible for diseases such as psoriasis and pemphigus. Even though these strategies have revolutionized the management of chronic dermatological conditions such as psoriasis, still there are a lot of unanswered questions. For instance, psoriasis patients respond very differently to each of the commercially available biological agents. This diversity could be partially explained by the differences in the sets of genes responsible for disease induction in each individual. Thus whole genome sequencing strategies, if feasible at individual levels, might help in tailoring these targeted treatments based on specific genetic backgrounds.Our intention in preparing this book was to explore the broad spectrum of the genetic aspects of immunological processes involved in cutaneous diseases. We have tried to cover most areas of dermatology where enough studies were available to gather a chapter. Still, there is a substantial lack of knowledge on the immunogenetics of many dermatological conditions. We hope that this book would encourage the investigators to fill these gaps of knowledge.

People living with a chronic illness are living with their own grief. But they are also living with so much more. We knew it was imperative to create a book that would adequately help the readers understand what it is like to live every day with the unknowns. We wanted the readers to recognize that chronic illness can be dark and dreary; we also wanted them to see that, despite the darkness, it is still possible to see the light and optimism in it all. This book was written to bring hope and encouragement to others living with chronic illness and bring understanding to those who know someone fighting every day to just make it through. There is no right or wrong way to read Invincible. Start at the beginning or jump right into a topic that speaks to you. All we ask is to be present while reading because these stories matter.

This book entitled "Nitric Oxide: From Research to Therapeutics" includes the most recent information about the role of Nitric Oxide (NO) in physiology, pathophysiology and its translation to therapeutics. The Editors are vastly experienced in the field of NO research. The international panels of contributors to the various chapters of the book are all well-established researchers and health professionals who have worked in different areas of NO physiology and pathophysiology, and have made a considerable impact in the field. The book is a comprehensive collection of the most recent information on NO as a physiological regulator and its diverse potential as a therapeutic target for the treatment for a variety of disease states. This compilation is aimed at professionals and students from both academia and the industry in the areas of basic biomedical and clinical sciences.

I'm just an average person who refused to let a diagnosis of an autoimmune disease define who I am or how I live. I've spent the last ten years extensively trialling and researching natural therapies and diet programs to overcome my condition, creating the foundation for my Autoimmune Warrior Protocol, which covers:Foods that heal and nourishFoods that trigger inflammationBenefit of natural therapiesLow-tox livingImportance of movement, meditation, sunshine, sleep, and self-care.From one autoimmune warrior to another: let me be the support for you on your health journey. I will guide you through the steps I took to reclaim my health, and I am confident they can work for you, too.

The concepts of the neuroendocrine system and the immune system emerged more or less simultaneously in the second half of the 20th century. Although these systems have a high degree of autonomy, it has also become clear that they interact in many ways and at different levels. This book focuses on the neuroendocrine and immune interactions that are fundamental to normal development and maintenance of health.The first introductory chapters are devoted to the historical and philosophical concepts within the field, as well as evolutionary considerations, offering critical interdisciplinary perspectives on the development of this field of research. Without attempting an exhaustive overview, the book then introduces some of the regulatory pathways that mediate interactions between the neuroendocrine and immune systems and examines modulating factors such as age and sex. In addition, several chapters address the importance of neuroendocrine-immune interactions in some disease states. Readers can expect to gain a broad perspective of neuroendocrine-immune interactions in development, health, and disease, along with a critical evaluation of current methods used in the field. Given its scope, the book is essential reading for undergraduate and graduate students with an interest in neuroendocrinology, neuroimmunology, and neuroscience, as well as postdoctoral fellows and established researchers seeking a comprehensive overview and historical perspective of the field of neuroendocrine-immune interactions.

The evolution of Antimicrobial Resistance is outpacing the ability to create novel antibiotics, which is proving to be a global problem in the fact of climate change, population growth, demographic shifts toward aging populations, emerging infections, and other crises. Therefore, clinicians are forced to use the existing antimicrobials more responsibly to preserve their longevity for generations to come. In a parallel vein, there has been a recent regulatory acknowledgement of the importance of prudent antimicrobial prescribing, mandating the implementation of Antimicrobial Stewardship across all healthcare settings. This is harder to manage in a non-traditional setting, which include long-term care facilities, outpatient care clinics, urgent care centers, and others. Given the trends in patient care in these settings¿particularly where the aging community is concerned¿this is proving to be a growing problem. This is troublesome even for spaces that have university and large hospital affiliations. There is a plethora of data describing the implementation and outcomes of Antimicrobial Stewardship Programs in acute-care hospitals with open-access, evidence-based guidelines endorsed by the Infectious Diseases Society of America. However, non-traditional settings often find themselves struggling to implement the same practices and procedures due to their unique needs and limited resources. There may be variability in access to resources, applicability, and patient-type. This book seeks to inform those in non-traditional healthcare settings on the principles, practice, and outcomes of Antimicrobial Stewardship Programs to improve patient care across all settings. The text begins by unpacking Antimicrobial Stewardship in nontraditional settings then covers specific environments, including long-term care facilities, outpatient clinics, and emergency departments. The text also covers special topics, including veterinary medicine, HIV, oncology, and immunocompromised patients, pediatrics, neonates, transplant, and critical care patients.

This book brings together what is currently known in terms of basic research in the field of long noncoding RNAs (lncRNAs) and builds on this to delve more deeply in the specific roles that lncRNAs are playing during inflammation. The book provides readers with basic knowledge on lncRNAs: from understanding the complexity of the transcriptome, conservation, structure and the tools used to investigate these aspects, to how we use this information to study lncRNAs in a specific biological context. The volume covers the emerging roles of lncRNAs in the initial stages of inflammation as well as their roles in specific inflammatory diseases including arthritis, lupus, diabetes and cardiovascular disease. The book also shows the emerging interest in using lncRNAs as a therapeutic target and how this could impact our ability to diagnose and treat inflammatory diseases in the future.

This volume contains up-to-date contributions written by leading experts in the role played by various microorganisms in psychiatric and neurological illness. The initial chapters present an evolutionary framework for the impact of microorganisms on behavior. This is followed by historical and epidemiological perspectives of the role of viruses in psychiatric illness. Subsequent chapters focus on different classes of microorganisms and psychiatric illnesses and emphasize diverse research approaches ranging from preclinical models to therapeutic interventions.

This fully revised and expanded second edition provides a comprehensive and up-to-date overview of nasal physiology and pathophysiology. With the help of numerous tables, schematic drawings, and color photographs, it helps readers gain a better understanding of the impact of structural changes and the process of disease development, and to make treatment decisions. Each chapter has been written by a leading expert in the field and addresses one important aspect in an accessible way. Covering all four functions of the nose: respiration, defense, olfaction and cosmesis, the book discusses the various techniques for the clinical evaluation of nasal function as well as current trends and future directions in nasal physiologic research. This second edition also includes additional chapters on rhinomanometry, local nasal inflammation, T cells and B cells, and artificial intelligence for the nose. Given its scope, the book is a valuable resource for both experienced otorhinolaryngologists and novices in the field.

This volume aims to help new investigators to the field by providing them with the protocols needed to set up clinically relevant animal models of fungal infection, as well as methods for in vivo immunology.Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and methods, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Antifungal Immunity: Methods and Protocols aims to be comprehensive guide for researchers in the field

Giant cell arteritis (GCA) is a systemic vasculitis that affects medium- to large-size arteries in which an inflammatory reaction destroys the media of the artery wall with the fragmentation of the elastic lamina, which can result in acute vision loss if not treated in a timely manner. The other common visual symptoms may include diplopia due to cranial nerve palsies and transient visual loss. This book provides a comprehensive overview of GCA, with chapters on epidemiology, cellular and molecular characteristics of the disease, extra-cranial involvement of GCA, medical imaging, and much more.

This volume provides inflammasome instructions and tips to study different aspects of inflammasome biology. Chapters guide readers through how to assess canonical and non-canonical inflammasome activation, in vitro immune and non-immune cells, whole blood, biochemical assays to evaluate ASC oligomerization, gasdermin D cleavage, and mapping phosphorylated residues of NLRP3. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, The Inflammasome: Methods and Protocols aims to provide protocols that will serve as tools for current and aspiring researchers interested in studying inflammasomes.

The aim of the book is to sensitize physicians and researchers to the important long-term health effects of early, persistent, and severe trauma. The author, an internist, rheumatologist, and basic researcher in psychoneuroimmunology, shows connections between adverse childhood experiences and typical adult sequelae. After early traumatic experiences and childhood stress, there is a higher incidence of mental illness, chronic pain, sleep disorders, dental problems, obesity, cardiovascular disease, asthma, diabetes mellitus and chronic inflammation. A selection of diseases unmistakably demonstrate the long-term consequences of early childhood trauma. These childhood experiences create a kind of long-term programming that has a negative effect in adulthood. From his psychoneuroimmunological perspective, Rainer Straub identifies four factors that link the brain to the immune system and are involved in chronic immune activation: direct connectors originating from the brain, indirect connectors functioning through hormonal and neuronal pathways, extracorporeal (the environmental factors) and pleiotropic connectors (genetic factors).The translation was done with the help of artificial intelligence. A subsequent human revision was done primarily in terms of content.