Practical laboratory manual- CELL BIOLOGY - Amit Gupta - Bog

Students should aware about its concept related to basic biology and also understands its theoretical concepts of cell Biology. The main objective of this manual course related to cell biology as given below- ¿ To understanding general laboratory rules and practices including safety in cell culture lab. ¿ Examination of samples (e.g. plant sections/animal cell or tissue) on a microscopic level and is totally depended on optical systems (calibrated ones) ¿ Cell culture characteristics: cell counting and analysis. ¿ To identify its cell structure at different magnification and measure its cell length including breadth using micrometry. ¿ To isolate DNA from various biological samples and also distinguish different blood cells. ¿ To understand and identified its stages of mitosis and meiosis. ¿ To understand and estimate chlorophyll content in plant samples. ¿ To demonstrate the concept of dialysis and osmosis.