Biovidenskab: generelle emner

This second edition provides new and updated chapters covering computational and mathematical techniques and concepts related to the field of comparative genomics. The topics covered in the chapters range from those that address general techniques and concepts that apply to all organisms to others that are specialized and apply to specific biological systems such as viruses, bacteria, nematodes, and insects. Well-known comparative genomics web-based platforms are also covered in specific chapters. Written in the highly successful Methods in Molecular Biology series format, many chapters include introductions to their respective topics and step-by-step comparison procedures, demonstrated on actual sets of genome sequences.Authoritative and cutting-edge, Comparative Genomics: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.

This detailed volume provides a comprehensive resource covering the existing and state-of-the-art tools in the field of profiling chromatin accessibility and its dynamics. Beginning with a section on bulk-cell methods for profiling chromatin accessibility and nucleosome positioning that rely on enzymatic cleavage of accessible DNA and produce information about relative accessibility, the book continues with methods that use single-molecule and enzymatic approaches to solving the problem of mapping absolute occupancy/accessibility, emerging tools for mapping DNA accessibility and nucleosome positioning in single cells, imaging-based methods for visualizing accessible chromatin in its nuclear context, as well as computational methods for the processing and analysis of chromatin accessibility datasets. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Chromatin Accessibility: Methods and Protocols serves as an extensive and useful reference for researchers studying different facets of chromatin accessibility in a wide variety of biological contexts. Chapter 6 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Artificial Intelligence in Biomedical and Modern Healthcare Informatics provides a deeper understanding of the current trends in AI and machine learning within healthcare diagnosis, its practical approach in healthcare, and gives insight into different wearable sensors and its device module to help doctors and their patients in enhanced healthcare system. The primary goal of this book is to detect difficulties and their solutions to medical practitioners for the early detection and prediction of any disease. The 56 chapters in the volume provide beginners and experts in the medical science field with general pictures and detailed descriptions of imaging and signal processing principles and clinical applications. With forefront applications and up-to-date analytical methods, this book captures the interests of colleagues in the medical imaging research field and is a valuable resource for healthcare professionals who wish to understand the principles and applications of signal and image processing and its related technologies in healthcare.

This book explores the potential of men¿s veganism to contest unsustainable anthropocentric masculinities. Examining what it means to be a vegan man and connections between men, masculinities and veganism, it addresses exploitative human-animal relations, climate change, and social inequalities as urgent and interconnected global issues. Using conceptual insights from critical studies on men and masculinities, ecofeminism, critical animal studies and vegan studies, this book examines the potential of men¿s veganism and vegan masculinities to foster more ethical, caring and sustainable ways of relating to nonhuman animals and to contribute towards more egalitarian gender relations. This book is grounded in a qualitative empirical study of the lived experiences of 61 vegan men in Northern Europe. The themes explored include men¿s transition to veganism, the emotional and embodied dimensions of men¿s veganism, negotiating social and intimate relationships as vegan men, and links between men¿s veganism, gender equality and social justice.

Here it is! This is the complete checklist of themammals of the Metazoic era. This era, which isbelieved to be the era after man, is full of interestinganimals leading interesting lives. Many are unlikeanything you see today. Others have similar relativesin today's world. This book is simply a list of thegenera and species of mammals thought up by theauthor, with the assistance of some of her friendsfamiliar with the concept of evolution. This book issolely to create a checklist of the species ofmammals the creator thought up for the Metazoicera. It is not a book of full-length details about eachmammal species or family.

A captivating exploration of how underwater animals tap into sound to survive, and a clarion call for humans to address the ways we invade these critical soundscapes - from an award-winning science writer.For centuries humans ignored sound in the 'silent world' of the ocean, assuming that what we couldn't perceive, didn't exist. But we couldn't have been more wrong. Marine scientists now have the technology to record and study the complex interplay of the myriad sounds in the sea. Finally, we can trace how sounds travel with the currents, bounce from the seafloor and surface, bend with temperature, and even saltiness; how sounds help marine life survive; and how human noise can transform entire marine ecosystems.In Sing Like Fish, award-winning science journalist Amorina Kingdon synthesises historical discoveries with the latest research in a clear and compelling portrait of this sonic undersea world. From plainfin midshipman fish, whose swim-bladder drumming is so loud it keeps houseboat-dwellers awake, to the syntax of whalesong, from the deafening crackle of snapping shrimp, to underwater earthquakes and volcanoes, sound plays a vital role in feeding, mating, parenting, navigating, and warning. Meanwhile, our seas also echo with human-made sound, and we are only just learning how these pervasive noises can mask mating calls, chase animals from their food, and even wound creatures.Intimate and artful, Sing Like Fish tells a uniquely complete story of ocean animals' submerged sounds, envisions a quieter future, and offers a profound new understanding of the world below the surface.

A detailed look at the neural and biological mechanisms of psychedelic use

Industrial crops offer farmers new market opportunities to increase their revenue by producing high-value products, focusing on fiber, forest, and energy crops, industrial oilseeds, rubber and resins, pharmaceuticals, and more. Technological innovations in agriculture have facilitated higher yields, but conserving crop genetic resources and diversity remains crucial for sustainable agricultural production. This poses a challenge that can be addressed through modern tools of biotechnology and genomics, utilizing the wealth of sequenced plant genomes. This book addresses the need for knowledge in managing the risks and conservation of genetic diversity associated with advanced technology. It provides comprehensive coverage of plant genomics and biotechnology, catering to post-graduate students, researchers, employees of seed and biotechnology companies, as well as instructors in plant genetics, breeding, and biotechnology fields.

This book explains the advancements of plant biotechnology and advanced molecular biology and explores the details of influential tools that complement conventional breeding and accelerate the development of plants resilient to adverse agroclimatic conditions and biofortified plants. Plant biotechnology from the basic sciences to current applications, such as pathway engineering, precursor feeding, transformation, elicitation with biotic and abiotic elicitors, and scaling up in bioreactors, have been included in these chapters to improve the production of secondary metabolites from different medicinal plants. It also highlights important factors often overlooked by methodologies used to develop plants' tolerance against biotic and abiotic stresses and in developing special foods, bio-chemicals, and pharmaceuticals. This book is valuable for researchers or students working on biosciences. It is also an updated and advanced reference material for the agriculture and pharmaceutical industries.

This book reviews key advances and new fundamentals in genetics. The increasing importance of genetic approaches in diverse areas of biology and medical sciences constantly requires in-depth information on genetic discoveries and research strategies for advanced graduate-level students as well as current researchers. This book focuses on genetic studies of various animal model systems and their major contributions to establishing modern genetics. Information covered in this book is mostly based on original research papers that extend from classical to modern genetics and applications. The contents are organized into four parts. Part I introduces fundamental concepts and experimental strategies in classical genetics. Part II discusses molecular genetics with transposons, transgenesis, clonal analysis, and gene editing technologies. Part III emphasizes epigenetic regulation of genome organization and gene expression. Part IV integrates earlier parts with landmark genetic studies on non-coding RNAs in dosage compensation, programmed cell death, growth control related to cancer, and behavioral neurobiology.

This book gives a complete overview of current developments in the implementation of high performance computing (HPC) in various biomimetic technologies. The book presents various topics that are subdivided into the following parts: A) biomimetic models and mechanics; B) locomotion and computational methods; C) distributed computing and its evolution; D) distributed and parallel computing architecture; E) high performance computing and biomimetics; F) big data, management, and visualization; and G) future of high performance computing in biomimetics. This book presents diverse computational technologies to model and replicate biologically inspired design for the purpose of solving complex human problems. The content of this book is presented in a simple and lucid style which can also be used by professionals, non-professionals, scientists, and students who are interested in the research area of high performance computing applications in the development of biomimetics technologies.

This third volume provides comprehensive protocols on pre-analytical, analytical, plasma, and serum proteomics. New and updated chapters are divided into nine sections, detailing blood processing and handling strategies, discovery- and targeted-based mass spectrometry, including workflows to aid in discovery and targeted data analysis, in addition to software and bioinformatics for the plasma proteome. This edition further integrates emerging areas in the development of technologies for plasma proteomics and assay platforms in biomarker discovery and translational proteomics, enrichment and detection strategies to understand the plasma proteome, and peptide, lipid and metabolite targeted assays. We also detail the emerging analysis of extracellular vesicles isolated from plasma. Written in the format of the highly successful Methods in Molecular Biology series, each of the 33 chapters includes an introduction to the topic, lists necessary materials and methods, includes hints and tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Serum/Plasma Proteomics: Methods and Protocols, Third Edition aims to be comprehensive guide for researchers.

This thorough volume presents a wide range of existing methods, from the very popular to the more exotic, in the area of plant genotyping. Many methods of plant genotyping were initially developed for medical research, but all genotyping methods, if they are to be successful, should be suitable for application across the full range of studies within plant biology, as seen in this collection. Plant genotyping methods herein are based on a variety of assessments, including DNA microarray, with its hundreds of thousands of simultaneous reactions, or separate individual studies of DNA sequencing and fragment analysis, PCR and qPCR, allele-specific molecular probes and primers, digestion with restriction endonucleases, microscopy and many others. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Genotyping: Methods and Protocols offers researchers the opportunity to update their knowledge and choose the most suitable method of plant genotyping for their chosen application.

This second edition provides new and updated protocols that can be used for generation of knockout animals. Chapters guide the reader through basic protocols for three genome editing technologies, target design tools, and specific protocols for each animal. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Genome Editing in Animals: Methods and Protocols, Second Edition aims to be a useful practical guide to researches to help further their study in this field.

This volume provides comprehensive dry and wet experiments, methods, and applications on nanopore sequencing. Chapters guide readers through bioinformatic procedures, genome sequencing, analysis of repetitive regions, structural variations, rapid and on-site microbial identification, epidemiology, and transcriptome analysis. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and methods, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Nanopore Sequencing: Methods and Protocols aims to be comprehensive guide for researchers.

Nongenetic Information and Evolution investigates the origin and nature of nongenetic information and its role in the mechanisms of evolutionary change. This book opens with an introduction to the theoretical background and forms of nongenetic information, alongside its relationship with genetic information. It then explores nongenetic information across the biological kingdoms, including animals, nonneural organisms, plants, and unicellulars. It also covers epigenetics as a form of nongenetic information, exploring DNA methylation, histone modifications, chromatin remodeling, and miRNA expression in this context. This book closes with a discussion of nongenetic information in evolution, considering evidence demonstrating its inheritance and hereditary impact. This book provides a detailed overview of the origin and evolutionary impact of nongenetic inheritance, delivering a basis for further understanding the occurrence of hereditary phenomena and transgenerational phenotypic changes that do not involve genes. This is a useful reference for researchers in the field of epigenetics, evolutionary biology, developmental biology, and cellular and molecular biology.

The Three Functional States of Proteins explores how structured proteins, intrinsically disordered proteins, and phase separated proteins contribute to the complexity of cellular life, and offers insights into their roles in both health and disease. It discusses the latest research findings and highlight groundbreaking discoveries and innovative methodologies used to study these protein states. Traditionally, the different states of proteins have been defined based on their structures and functions. However, it is becoming increasingly clear that these criteria alone may not be sufficient to capture the complex and multifaceted properties of these molecules. Definitions based on thermodynamics and kinetics are now recognized as potentially more appropriate for comprehensively understanding protein states. Emerging evidence indicates that under physiological conditions, a majority of proteins possess the capability to exist in and transition between the native, droplet, and amyloid states. These distinct states play crucial roles in various cellular functions, influenced significantly by their physicochemical and structural properties. The book also considers the interactions among these states and discusses how their internal organization as individual molecules, as well as their collective organization as molecular assemblies are stabilized. Furthermore, it examines the processes by which these states are formed and the cellular functions associated with each specific state.

The Physics of Protein Structure and Dynamics looks at various aspects of protein structure and dynamics from a physico-chemical point of view. It goes into some depth regarding the description of non-covalent forces that determine the relative stability of folded and unfolded proteins. Anharmonic protein dynamics involving motions between different minima of a rugged Gibbs energy landscape is described in great detail. The book combines various aspects of the protein folding/unfolding processes with an overview of intrinsically disordered proteins, which have attracted considerable interest of the protein community over the last 25 years but are thus far underrepresented in classroom-oriented textbooks. The book looks at protein folding and intrinsically disordered proteins as heavily interrelated topics that need to be viewed together. Furthermore, it presents some basic physico-chemical aspects of protein/peptide self-assembly into nanoscale fibrils. Intrinsically disordered peptides and proteins play a major role particularly in aggregation and self-assembly processes that lead to various diseases (Alzheimer, Parkinson, Huntington, Mad-Cow). Therefore, the relevance of protein disorder for protein self-assembly deserves a closer look. Protein self-assembly cannot be separated from protein folding since it is frequently the product of misfolding. With regard to modern theories, the folding processes are linked to insights on protein dynamics and the discovered relationship between proteins and spin glasses.

This edited volume covers sustainable biomass valorization and its application in various sectors.Bioenergy has concerned enormous attention as a promising alternative to conventional energy resources. Abundance of bioresources and their renewable nature provides a distinct advantage to biofuels from sustainability perspective. Biofuels have found increasing applications in the energy and transportation sector in recent years. Blended fuels are being used worldwide. However, several challenges remain to be addressed due to the diverse nature of biomasses and inherent recalcitrant nature of biological materials used as feedstock. This book aims to provide an overview of recent developments in the production of bioenergy and its applications in various areas. Specific emphasis is given to production of biofuels from modern biomass such as agro-forest waste and dedicated non-edible energy crops that do not interfere with food production. The scope of the book covers synthesis and characterization of biologically derived fuels, advances in microbiological methods and enzyme engineering approaches. The book covers techno-economic analysis to identify challenges and prospects for commercialization of biofuels. The directions for future research should pave way for net zero scenario for a sustainable world.The book will cater to a wide range of readers interested in learning the fundamentals as well as advances in the field of biofuels.

Osteomyelitis and Discitis of the Spine: A Guide for the Management of Infectious Etiology of the Vertebral Column and Neurological Components examines these spinal conditions and provides readers with the most appropriate management plans, including surgical treatment together with its indications. This book guides spine neurosurgeons and others when choosing the optimal surgical approach for their patients, shedding light on current advances in surgical techniques used in the management of vertebral osteomyelitis and discitis, particularly minimally invasive spine approaches. With authors representing diverse specialties, chapters reflect current clinical and surgical practice and discuss pathophysiology and pain management and rehabilitation. The book begins with related anatomy and extends to post-management rehabilitation and pain control, including a chapter dedicated to pediatric patients and their differing causative organisms, etiology, diagnostic modalities, and pathophysiology. This reference will provide increased awareness of potential spinal infection, facilitating multidisciplinary management plans, improving patient safety and outcomes, and enabling early intervention alternatives that improve patient outcomes and long-term quality of life.

"An overview of how and why human males evolved the capacity to be highly involved caregivers, and why some are more involved than others"--

By 1930, no place in the world was less well explored than Greenland. The native Inuit had occupied the relatively accessible west coast for centuries. The east coast, however, was another story. In August 1930, Henry George Watkins (nicknamed "Gino"), a twenty-three-year-old British explorer, led thirteen scientists and explorers on an ambitious expedition to the east coast of Greenland and into its vast and forbidding interior to set up a permanent meteorological base on the icecap, 8,200 feet above sea level. The Ice Cap Station was to be the anchor of a transpolar route of air travel from Europe to North America.The weather on the ice cap was appalling. Fierce storms. Temperatures plunging lower than -45° Celsius in the winter. Watkins's scheme called for rotating teams of two men each to monitor the station for two months at a time. No one had ever tried to winter over in that hostile landscape, let alone manage a weather station through twelve continuous months. Watkins was younger than anyone under his command. But he had several daring trips to the Arctic under his belt and no one doubted his judgement.The first crisis came in the fall when a snowstorm stranded a resupply mission halfway to the top for many weeks. When they arrived at the ice cap, there were not enough provisions and fuel for another two-man shift, so the station would have to be abandoned. Then team member August Courtauld made an astonishing offer. To enable the mission to go forward, he would monitor the station solo through the winter. When a team went up in March to relieve Courtauld, after weeks of brutal effort to make the 130-mile journey, they could find no trace of him or the station. By the end of March, Courtauld's situation was desperate. He was buried under an immovable load of frozen snow and was disastrously short on supplies. On 21 April, four months after Courtauld began his solitary vigil, Gino Watkins set out inland with two companions to find and rescue him.David Roberts, "veteran mountain climber and chronicler of adventures" (The Washington Post), draws on firsthand accounts and archival materials to tell the story of this daring expedition and of the epic survival ordeal that ensued.